Experiment: MoYLS4 with DMSO 10%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sctV and DVUA0104.1 overlap by 4 nucleotides DVUA0104.1 and DORF831 are separated by 2 nucleotides DORF831 and DVUA0105 overlap by 4 nucleotides DVUA0105 and DVUA0106 are separated by 2 nucleotides
DVUA0104: sctV - type III secretion inner membrane protein, HrcV family (TIGR), at 140,328 to 142,421
sctV
DVUA0104.1: DVUA0104.1 - no description, at 142,418 to 142,777
DVUA0104.1
DORF831: DORF831 - SEED: FIG00602593: hypothetical protein, at 142,780 to 143,148
DORF831
DVUA0105: DVUA0105 - conserved hypothetical protein (TIGR), at 143,145 to 143,519
DVUA0105
DVUA0106: DVUA0106 - type III secretion target, YopN family (TIGR), at 143,522 to 144,646
DVUA0106
Position (kb)
142
143
144 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 141.784 kb on + strand, within sctV at 141.784 kb on + strand, within sctV at 141.909 kb on - strand, within sctV at 141.961 kb on + strand, within sctV at 142.040 kb on - strand, within sctV at 142.112 kb on - strand, within sctV at 142.147 kb on - strand, within sctV at 142.147 kb on - strand, within sctV at 142.161 kb on + strand, within sctV at 142.169 kb on - strand, within sctV at 142.169 kb on - strand, within sctV at 142.396 kb on + strand at 142.427 kb on - strand at 142.427 kb on - strand at 142.427 kb on - strand at 142.453 kb on + strand at 142.522 kb on - strand, within DVUA0104.1 at 142.605 kb on - strand, within DVUA0104.1 at 142.627 kb on + strand, within DVUA0104.1 at 142.640 kb on - strand, within DVUA0104.1 at 142.696 kb on + strand, within DVUA0104.1 at 142.731 kb on - strand, within DVUA0104.1 at 142.786 kb on - strand at 143.040 kb on - strand, within DORF831 at 143.275 kb on + strand, within DVUA0105 at 143.345 kb on - strand, within DVUA0105 at 143.475 kb on - strand, within DVUA0105 at 143.508 kb on + strand at 143.516 kb on - strand at 143.519 kb on + strand at 143.572 kb on - strand at 143.575 kb on - strand at 143.578 kb on - strand at 143.579 kb on + strand at 143.597 kb on - strand at 143.785 kb on + strand, within DVUA0106 at 143.806 kb on + strand, within DVUA0106 at 143.814 kb on - strand, within DVUA0106 at 143.814 kb on - strand, within DVUA0106 at 143.833 kb on + strand, within DVUA0106 at 143.892 kb on - strand, within DVUA0106 at 143.906 kb on + strand, within DVUA0106 at 143.906 kb on + strand, within DVUA0106 at 143.914 kb on - strand, within DVUA0106 at 143.916 kb on - strand, within DVUA0106 at 143.962 kb on - strand, within DVUA0106 at 144.009 kb on + strand, within DVUA0106 at 144.050 kb on + strand, within DVUA0106 at 144.090 kb on - strand, within DVUA0106 at 144.148 kb on + strand, within DVUA0106
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with DMSO 10% remove 141,784 + sctV DVUA0104 0.70 +0.1 141,784 + sctV DVUA0104 0.70 -3.6 141,909 - sctV DVUA0104 0.76 +0.7 141,961 + sctV DVUA0104 0.78 -0.1 142,040 - sctV DVUA0104 0.82 -0.1 142,112 - sctV DVUA0104 0.85 +0.2 142,147 - sctV DVUA0104 0.87 +0.3 142,147 - sctV DVUA0104 0.87 -0.6 142,161 + sctV DVUA0104 0.88 -0.6 142,169 - sctV DVUA0104 0.88 -0.1 142,169 - sctV DVUA0104 0.88 -2.0 142,396 + +1.6 142,427 - +1.4 142,427 - +0.8 142,427 - -0.3 142,453 + +0.1 142,522 - DVUA0104.1 0.29 +0.6 142,605 - DVUA0104.1 0.52 -0.5 142,627 + DVUA0104.1 0.58 +0.1 142,640 - DVUA0104.1 0.62 +0.1 142,696 + DVUA0104.1 0.77 -0.8 142,731 - DVUA0104.1 0.87 -0.4 142,786 - +2.2 143,040 - DORF831 0.70 -1.1 143,275 + DVUA0105 0.35 -1.3 143,345 - DVUA0105 0.53 -0.2 143,475 - DVUA0105 0.88 -0.3 143,508 + -0.1 143,516 - -0.1 143,519 + +0.6 143,572 - -0.9 143,575 - +0.2 143,578 - +0.6 143,579 + +0.1 143,597 - -0.5 143,785 + DVUA0106 0.23 +0.1 143,806 + DVUA0106 0.25 -0.7 143,814 - DVUA0106 0.26 +0.8 143,814 - DVUA0106 0.26 -0.2 143,833 + DVUA0106 0.28 -0.1 143,892 - DVUA0106 0.33 +0.0 143,906 + DVUA0106 0.34 -0.5 143,906 + DVUA0106 0.34 -0.1 143,914 - DVUA0106 0.35 -0.8 143,916 - DVUA0106 0.35 -1.1 143,962 - DVUA0106 0.39 -0.0 144,009 + DVUA0106 0.43 -1.0 144,050 + DVUA0106 0.47 +1.2 144,090 - DVUA0106 0.50 -2.4 144,148 + DVUA0106 0.56 +0.6
Or see this region's nucleotide sequence