Experiment: MoYLS4 with Cefoxitin 5ug/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0155 and pcrA overlap by 4 nucleotides pcrA and thiL overlap by 4 nucleotides thiL and DVU0158 are separated by 20 nucleotides
DVU0155: DVU0155 - type I phosphodiesterase/nucleotide pyrophosphatase family protein (TIGR), at 190,855 to 192,243
DVU0155
DVU0156: pcrA - ATP-dependent DNA helicase, UvrD/REP family (TIGR), at 192,240 to 194,399
pcrA
DVU0157: thiL - thiamin-monophosphate kinase (TIGR), at 194,396 to 195,367
thiL
DVU0158: DVU0158 - conserved hypothetical protein TIGR00104 (TIGR), at 195,388 to 196,386
DVU0158
Position (kb)
192
193
194
195 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 191.242 kb on + strand, within DVU0155 at 191.282 kb on - strand, within DVU0155 at 191.397 kb on + strand, within DVU0155 at 191.447 kb on + strand, within DVU0155 at 191.479 kb on - strand, within DVU0155 at 191.529 kb on + strand, within DVU0155 at 191.529 kb on + strand, within DVU0155 at 191.580 kb on - strand, within DVU0155 at 191.580 kb on - strand, within DVU0155 at 191.595 kb on + strand, within DVU0155 at 191.755 kb on + strand, within DVU0155 at 191.861 kb on + strand, within DVU0155 at 191.900 kb on - strand, within DVU0155 at 191.938 kb on + strand, within DVU0155 at 191.977 kb on - strand, within DVU0155 at 191.979 kb on + strand, within DVU0155 at 191.987 kb on - strand, within DVU0155 at 192.054 kb on + strand, within DVU0155 at 192.064 kb on - strand, within DVU0155 at 192.070 kb on + strand, within DVU0155 at 192.076 kb on - strand, within DVU0155 at 192.156 kb on - strand at 192.197 kb on + strand at 192.197 kb on + strand at 192.197 kb on + strand at 192.197 kb on + strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.205 kb on - strand at 192.283 kb on - strand at 192.311 kb on + strand at 192.369 kb on - strand at 192.402 kb on - strand at 192.474 kb on + strand, within pcrA at 192.474 kb on + strand, within pcrA at 192.474 kb on + strand, within pcrA at 192.552 kb on - strand, within pcrA at 192.575 kb on - strand, within pcrA at 192.597 kb on + strand, within pcrA at 192.603 kb on - strand, within pcrA at 192.605 kb on + strand, within pcrA at 192.613 kb on - strand, within pcrA at 192.636 kb on + strand, within pcrA at 192.643 kb on - strand, within pcrA at 192.671 kb on - strand, within pcrA at 192.671 kb on - strand, within pcrA at 192.714 kb on + strand, within pcrA at 192.714 kb on + strand, within pcrA at 192.722 kb on - strand, within pcrA at 192.771 kb on + strand, within pcrA at 192.798 kb on + strand, within pcrA at 192.806 kb on - strand, within pcrA at 192.849 kb on - strand, within pcrA at 192.931 kb on - strand, within pcrA at 192.977 kb on + strand, within pcrA at 192.985 kb on - strand, within pcrA at 193.116 kb on + strand, within pcrA at 193.157 kb on + strand, within pcrA at 193.172 kb on + strand, within pcrA at 193.172 kb on + strand, within pcrA at 193.180 kb on - strand, within pcrA at 193.188 kb on - strand, within pcrA at 193.216 kb on - strand, within pcrA at 193.262 kb on + strand, within pcrA at 193.279 kb on - strand, within pcrA at 193.315 kb on - strand, within pcrA at 193.325 kb on + strand, within pcrA at 193.448 kb on - strand, within pcrA at 193.502 kb on + strand, within pcrA at 193.503 kb on - strand, within pcrA at 193.510 kb on - strand, within pcrA at 193.512 kb on + strand, within pcrA at 193.560 kb on + strand, within pcrA at 193.560 kb on + strand, within pcrA at 193.568 kb on - strand, within pcrA at 193.568 kb on - strand, within pcrA at 193.568 kb on - strand, within pcrA at 193.597 kb on + strand, within pcrA at 193.597 kb on + strand, within pcrA at 193.605 kb on - strand, within pcrA at 193.605 kb on - strand, within pcrA at 193.605 kb on - strand, within pcrA at 193.640 kb on - strand, within pcrA at 193.657 kb on - strand, within pcrA at 193.691 kb on + strand, within pcrA at 193.726 kb on + strand, within pcrA at 193.734 kb on - strand, within pcrA at 193.757 kb on - strand, within pcrA at 193.758 kb on - strand, within pcrA at 193.841 kb on + strand, within pcrA at 193.872 kb on - strand, within pcrA at 193.904 kb on + strand, within pcrA at 193.934 kb on - strand, within pcrA at 193.985 kb on - strand, within pcrA at 194.031 kb on - strand, within pcrA at 194.068 kb on + strand, within pcrA at 194.125 kb on - strand, within pcrA at 194.282 kb on - strand at 194.299 kb on - strand at 194.351 kb on + strand at 195.379 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Cefoxitin 5ug/mL remove 191,242 + DVU0155 0.28 -0.2 191,282 - DVU0155 0.31 -0.5 191,397 + DVU0155 0.39 -0.1 191,447 + DVU0155 0.43 -0.1 191,479 - DVU0155 0.45 -0.3 191,529 + DVU0155 0.49 -0.4 191,529 + DVU0155 0.49 +0.4 191,580 - DVU0155 0.52 -0.8 191,580 - DVU0155 0.52 -1.7 191,595 + DVU0155 0.53 +0.7 191,755 + DVU0155 0.65 -0.5 191,861 + DVU0155 0.72 -0.1 191,900 - DVU0155 0.75 +0.3 191,938 + DVU0155 0.78 -0.4 191,977 - DVU0155 0.81 -0.2 191,979 + DVU0155 0.81 -0.1 191,987 - DVU0155 0.81 -0.5 192,054 + DVU0155 0.86 -0.6 192,064 - DVU0155 0.87 -1.2 192,070 + DVU0155 0.87 -3.2 192,076 - DVU0155 0.88 -0.1 192,156 - -0.7 192,197 + +0.1 192,197 + +0.1 192,197 + -0.1 192,197 + +0.8 192,205 - +0.8 192,205 - -0.1 192,205 - -0.9 192,205 - +1.5 192,205 - -0.5 192,205 - -1.5 192,205 - -0.1 192,283 - +1.0 192,311 + -0.1 192,369 - +0.0 192,402 - -0.2 192,474 + pcrA DVU0156 0.11 +0.1 192,474 + pcrA DVU0156 0.11 -0.5 192,474 + pcrA DVU0156 0.11 -0.9 192,552 - pcrA DVU0156 0.14 -0.5 192,575 - pcrA DVU0156 0.16 -0.1 192,597 + pcrA DVU0156 0.17 -0.9 192,603 - pcrA DVU0156 0.17 +0.5 192,605 + pcrA DVU0156 0.17 -0.1 192,613 - pcrA DVU0156 0.17 +0.2 192,636 + pcrA DVU0156 0.18 -0.3 192,643 - pcrA DVU0156 0.19 +0.1 192,671 - pcrA DVU0156 0.20 -0.5 192,671 - pcrA DVU0156 0.20 -0.6 192,714 + pcrA DVU0156 0.22 -0.0 192,714 + pcrA DVU0156 0.22 +0.3 192,722 - pcrA DVU0156 0.22 -0.7 192,771 + pcrA DVU0156 0.25 -0.4 192,798 + pcrA DVU0156 0.26 -1.1 192,806 - pcrA DVU0156 0.26 -0.4 192,849 - pcrA DVU0156 0.28 +0.5 192,931 - pcrA DVU0156 0.32 -0.4 192,977 + pcrA DVU0156 0.34 -0.3 192,985 - pcrA DVU0156 0.34 -0.4 193,116 + pcrA DVU0156 0.41 -1.1 193,157 + pcrA DVU0156 0.42 -0.1 193,172 + pcrA DVU0156 0.43 +0.1 193,172 + pcrA DVU0156 0.43 -0.4 193,180 - pcrA DVU0156 0.44 -1.3 193,188 - pcrA DVU0156 0.44 -0.9 193,216 - pcrA DVU0156 0.45 -0.3 193,262 + pcrA DVU0156 0.47 +0.9 193,279 - pcrA DVU0156 0.48 -0.4 193,315 - pcrA DVU0156 0.50 -1.4 193,325 + pcrA DVU0156 0.50 +0.0 193,448 - pcrA DVU0156 0.56 -0.2 193,502 + pcrA DVU0156 0.58 -0.9 193,503 - pcrA DVU0156 0.58 -1.3 193,510 - pcrA DVU0156 0.59 -0.0 193,512 + pcrA DVU0156 0.59 -0.6 193,560 + pcrA DVU0156 0.61 +0.9 193,560 + pcrA DVU0156 0.61 -0.7 193,568 - pcrA DVU0156 0.61 -0.7 193,568 - pcrA DVU0156 0.61 -0.3 193,568 - pcrA DVU0156 0.61 -0.4 193,597 + pcrA DVU0156 0.63 -0.3 193,597 + pcrA DVU0156 0.63 -0.5 193,605 - pcrA DVU0156 0.63 -0.2 193,605 - pcrA DVU0156 0.63 -0.7 193,605 - pcrA DVU0156 0.63 -0.9 193,640 - pcrA DVU0156 0.65 -0.8 193,657 - pcrA DVU0156 0.66 -0.2 193,691 + pcrA DVU0156 0.67 +0.3 193,726 + pcrA DVU0156 0.69 -1.3 193,734 - pcrA DVU0156 0.69 -0.3 193,757 - pcrA DVU0156 0.70 -1.8 193,758 - pcrA DVU0156 0.70 -0.7 193,841 + pcrA DVU0156 0.74 -0.3 193,872 - pcrA DVU0156 0.76 +0.5 193,904 + pcrA DVU0156 0.77 -0.4 193,934 - pcrA DVU0156 0.78 -1.1 193,985 - pcrA DVU0156 0.81 -0.9 194,031 - pcrA DVU0156 0.83 -0.3 194,068 + pcrA DVU0156 0.85 -0.7 194,125 - pcrA DVU0156 0.87 -1.6 194,282 - +0.7 194,299 - -1.4 194,351 + -0.3 195,379 + -1.6
Or see this region's nucleotide sequence