Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS04620

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS04610 and ECOLIN_RS04615 overlap by 4 nucleotidesECOLIN_RS04615 and ECOLIN_RS04620 overlap by 4 nucleotidesECOLIN_RS04620 and ECOLIN_RS04625 are separated by 98 nucleotides ECOLIN_RS04610: ECOLIN_RS04610 - heavy metal-binding domain-containing protein, at 931,398 to 931,721 _RS04610 ECOLIN_RS04615: ECOLIN_RS04615 - N-acetylmuramoyl-L-alanine amidase AmiD, at 931,718 to 932,548 _RS04615 ECOLIN_RS04620: ECOLIN_RS04620 - NAD(P)-dependent oxidoreductase, at 932,545 to 933,558 _RS04620 ECOLIN_RS04625: ECOLIN_RS04625 - SDR family oxidoreductase, at 933,657 to 935,087 _RS04625 Position (kb) 932 933 934Strain fitness (log2 ratio) -2 -1 0 1at 931.673 kb on + strand, within ECOLIN_RS04610at 931.932 kb on - strand, within ECOLIN_RS04615at 932.153 kb on - strand, within ECOLIN_RS04615at 932.324 kb on + strand, within ECOLIN_RS04615at 932.629 kb on + strandat 934.212 kb on - strand, within ECOLIN_RS04625

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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931,673 + ECOLIN_RS04610 0.85 -1.9
931,932 - ECOLIN_RS04615 0.26 -0.5
932,153 - ECOLIN_RS04615 0.52 +0.6
932,324 + ECOLIN_RS04615 0.73 +0.3
932,629 + +0.5
934,212 - ECOLIN_RS04625 0.39 -0.6

Or see this region's nucleotide sequence