Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS00585

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS00570 and ECOLIN_RS00575 are separated by 9 nucleotidesECOLIN_RS00575 and ECOLIN_RS00585 are separated by 203 nucleotidesECOLIN_RS00585 and ECOLIN_RS00590 are separated by 87 nucleotidesECOLIN_RS00590 and ECOLIN_RS00595 overlap by 4 nucleotides ECOLIN_RS00570: ECOLIN_RS00570 - type II secretion system protein GspE, at 118,799 to 120,184 _RS00570 ECOLIN_RS00575: ECOLIN_RS00575 - prepilin peptidase-dependent pilin, at 120,194 to 120,634 _RS00575 ECOLIN_RS00585: ECOLIN_RS00585 - carboxylating nicotinate-nucleotide diphosphorylase, at 120,838 to 121,731 _RS00585 ECOLIN_RS00590: ECOLIN_RS00590 - 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD, at 121,819 to 122,370 _RS00590 ECOLIN_RS00595: ECOLIN_RS00595 - beta-lactamase regulator AmpE, at 122,367 to 123,221 _RS00595 Position (kb) 120 121 122Strain fitness (log2 ratio) -1 0 1 2at 120.179 kb on - strandat 120.195 kb on + strandat 120.269 kb on - strand, within ECOLIN_RS00575at 120.269 kb on - strand, within ECOLIN_RS00575at 120.447 kb on - strand, within ECOLIN_RS00575at 120.447 kb on - strand, within ECOLIN_RS00575at 120.507 kb on - strand, within ECOLIN_RS00575at 120.507 kb on - strand, within ECOLIN_RS00575at 120.507 kb on - strand, within ECOLIN_RS00575at 120.538 kb on - strand, within ECOLIN_RS00575at 120.901 kb on - strandat 121.096 kb on + strand, within ECOLIN_RS00585at 121.674 kb on + strandat 121.716 kb on - strandat 121.746 kb on + strandat 121.760 kb on + strandat 121.776 kb on + strandat 121.776 kb on + strandat 121.777 kb on - strandat 121.803 kb on + strandat 121.804 kb on - strandat 121.804 kb on - strandat 121.804 kb on - strandat 121.874 kb on - strandat 121.983 kb on + strand, within ECOLIN_RS00590at 122.069 kb on + strand, within ECOLIN_RS00590at 122.070 kb on - strand, within ECOLIN_RS00590at 122.078 kb on - strand, within ECOLIN_RS00590at 122.265 kb on + strand, within ECOLIN_RS00590at 122.396 kb on - strandat 122.557 kb on + strand, within ECOLIN_RS00595at 122.646 kb on + strand, within ECOLIN_RS00595at 122.646 kb on + strand, within ECOLIN_RS00595at 122.666 kb on + strand, within ECOLIN_RS00595

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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120,179 - +0.7
120,195 + +0.1
120,269 - ECOLIN_RS00575 0.17 +0.6
120,269 - ECOLIN_RS00575 0.17 +0.3
120,447 - ECOLIN_RS00575 0.57 -0.4
120,447 - ECOLIN_RS00575 0.57 -0.6
120,507 - ECOLIN_RS00575 0.71 -0.3
120,507 - ECOLIN_RS00575 0.71 +0.1
120,507 - ECOLIN_RS00575 0.71 -0.1
120,538 - ECOLIN_RS00575 0.78 +0.4
120,901 - +0.4
121,096 + ECOLIN_RS00585 0.29 +0.2
121,674 + -1.5
121,716 - +0.2
121,746 + +0.2
121,760 + +0.1
121,776 + +1.6
121,776 + -0.7
121,777 - +1.1
121,803 + +0.1
121,804 - +1.0
121,804 - +1.5
121,804 - +1.0
121,874 - +1.6
121,983 + ECOLIN_RS00590 0.30 +1.9
122,069 + ECOLIN_RS00590 0.45 +0.7
122,070 - ECOLIN_RS00590 0.45 +1.9
122,078 - ECOLIN_RS00590 0.47 +0.7
122,265 + ECOLIN_RS00590 0.81 +1.5
122,396 - +0.5
122,557 + ECOLIN_RS00595 0.22 +0.3
122,646 + ECOLIN_RS00595 0.33 +0.4
122,646 + ECOLIN_RS00595 0.33 -0.5
122,666 + ECOLIN_RS00595 0.35 -0.2

Or see this region's nucleotide sequence