Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS00020

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS00010 and ECOLIN_RS00015 are separated by 1 nucleotidesECOLIN_RS00015 and ECOLIN_RS00020 are separated by 0 nucleotidesECOLIN_RS00020 and ECOLIN_RS00030 are separated by 213 nucleotidesECOLIN_RS00030 and ECOLIN_RS00035 are separated by 59 nucleotides ECOLIN_RS00010: ECOLIN_RS00010 - bifunctional aspartate kinase/homoserine dehydrogenase I, at 361 to 2,823 _RS00010 ECOLIN_RS00015: ECOLIN_RS00015 - homoserine kinase, at 2,825 to 3,757 _RS00015 ECOLIN_RS00020: ECOLIN_RS00020 - threonine synthase, at 3,758 to 5,044 _RS00020 ECOLIN_RS00030: ECOLIN_RS00030 - DUF2502 domain-containing protein, at 5,258 to 5,554 _RS00030 ECOLIN_RS00035: ECOLIN_RS00035 - peroxide stress protein YaaA, at 5,614 to 6,390 _RS00035 Position (kb) 3 4 5 6Strain fitness (log2 ratio) -3 -2 -1 0 1at 2.796 kb on + strandat 2.928 kb on + strand, within ECOLIN_RS00015at 3.021 kb on - strand, within ECOLIN_RS00015at 3.021 kb on - strand, within ECOLIN_RS00015at 3.467 kb on + strand, within ECOLIN_RS00015at 4.270 kb on + strand, within ECOLIN_RS00020at 4.270 kb on + strand, within ECOLIN_RS00020at 4.270 kb on + strand, within ECOLIN_RS00020at 4.271 kb on - strand, within ECOLIN_RS00020at 4.271 kb on - strand, within ECOLIN_RS00020at 4.565 kb on - strand, within ECOLIN_RS00020at 4.740 kb on - strand, within ECOLIN_RS00020at 4.824 kb on + strand, within ECOLIN_RS00020at 5.276 kb on - strandat 5.439 kb on - strand, within ECOLIN_RS00030at 5.439 kb on - strand, within ECOLIN_RS00030at 5.439 kb on - strand, within ECOLIN_RS00030at 5.494 kb on + strand, within ECOLIN_RS00030at 5.783 kb on - strand, within ECOLIN_RS00035at 5.888 kb on - strand, within ECOLIN_RS00035at 5.894 kb on - strand, within ECOLIN_RS00035at 5.944 kb on - strand, within ECOLIN_RS00035

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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2,796 + +0.1
2,928 + ECOLIN_RS00015 0.11 -0.3
3,021 - ECOLIN_RS00015 0.21 -0.5
3,021 - ECOLIN_RS00015 0.21 -3.4
3,467 + ECOLIN_RS00015 0.69 -2.0
4,270 + ECOLIN_RS00020 0.40 -0.2
4,270 + ECOLIN_RS00020 0.40 +0.0
4,270 + ECOLIN_RS00020 0.40 -0.3
4,271 - ECOLIN_RS00020 0.40 -0.4
4,271 - ECOLIN_RS00020 0.40 +0.3
4,565 - ECOLIN_RS00020 0.63 +0.3
4,740 - ECOLIN_RS00020 0.76 -0.5
4,824 + ECOLIN_RS00020 0.83 +0.1
5,276 - +0.1
5,439 - ECOLIN_RS00030 0.61 -2.2
5,439 - ECOLIN_RS00030 0.61 -0.3
5,439 - ECOLIN_RS00030 0.61 +0.6
5,494 + ECOLIN_RS00030 0.79 -0.2
5,783 - ECOLIN_RS00035 0.22 -0.0
5,888 - ECOLIN_RS00035 0.35 -3.7
5,894 - ECOLIN_RS00035 0.36 -0.2
5,944 - ECOLIN_RS00035 0.42 -0.2

Or see this region's nucleotide sequence