Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20690

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20685 and ECOLIN_RS20690 are separated by 15 nucleotidesECOLIN_RS20690 and ECOLIN_RS20695 overlap by 8 nucleotides ECOLIN_RS20685: ECOLIN_RS20685 - lipopolysaccharide 3-alpha-galactosyltransferase, at 4,168,457 to 4,169,473 _RS20685 ECOLIN_RS20690: ECOLIN_RS20690 - lipopolysaccharide core heptose(I) kinase RfaP, at 4,169,489 to 4,170,286 _RS20690 ECOLIN_RS20695: ECOLIN_RS20695 - glycosyltransferase family 4 protein, at 4,170,279 to 4,171,403 _RS20695 Position (kb) 4169 4170 4171Strain fitness (log2 ratio) -1 0 1 2 3 4 5at 4168.489 kb on + strandat 4169.040 kb on - strand, within ECOLIN_RS20685at 4169.040 kb on - strand, within ECOLIN_RS20685at 4169.126 kb on + strand, within ECOLIN_RS20685at 4169.140 kb on - strand, within ECOLIN_RS20685at 4169.150 kb on - strand, within ECOLIN_RS20685at 4169.219 kb on - strand, within ECOLIN_RS20685at 4169.219 kb on - strand, within ECOLIN_RS20685at 4169.223 kb on + strand, within ECOLIN_RS20685at 4169.311 kb on + strand, within ECOLIN_RS20685at 4169.312 kb on - strand, within ECOLIN_RS20685at 4169.456 kb on - strandat 4169.733 kb on - strand, within ECOLIN_RS20690at 4169.794 kb on + strand, within ECOLIN_RS20690at 4169.796 kb on + strand, within ECOLIN_RS20690at 4169.860 kb on + strand, within ECOLIN_RS20690at 4169.861 kb on - strand, within ECOLIN_RS20690at 4169.867 kb on - strand, within ECOLIN_RS20690at 4170.010 kb on - strand, within ECOLIN_RS20690at 4170.013 kb on + strand, within ECOLIN_RS20690at 4170.013 kb on + strand, within ECOLIN_RS20690at 4170.013 kb on + strand, within ECOLIN_RS20690at 4170.014 kb on - strand, within ECOLIN_RS20690at 4170.014 kb on - strand, within ECOLIN_RS20690at 4170.014 kb on - strand, within ECOLIN_RS20690at 4170.014 kb on - strand, within ECOLIN_RS20690at 4170.108 kb on - strand, within ECOLIN_RS20690at 4170.275 kb on - strandat 4170.332 kb on - strandat 4170.333 kb on + strandat 4170.334 kb on - strandat 4170.479 kb on + strand, within ECOLIN_RS20695at 4170.481 kb on + strand, within ECOLIN_RS20695at 4170.482 kb on - strand, within ECOLIN_RS20695at 4170.546 kb on + strand, within ECOLIN_RS20695at 4170.603 kb on + strand, within ECOLIN_RS20695at 4170.709 kb on + strand, within ECOLIN_RS20695at 4170.709 kb on + strand, within ECOLIN_RS20695at 4170.711 kb on + strand, within ECOLIN_RS20695at 4170.711 kb on + strand, within ECOLIN_RS20695at 4170.711 kb on + strand, within ECOLIN_RS20695at 4170.711 kb on + strand, within ECOLIN_RS20695at 4170.711 kb on + strand, within ECOLIN_RS20695at 4170.712 kb on - strand, within ECOLIN_RS20695at 4170.712 kb on - strand, within ECOLIN_RS20695at 4170.712 kb on - strand, within ECOLIN_RS20695at 4170.712 kb on - strand, within ECOLIN_RS20695at 4170.714 kb on + strand, within ECOLIN_RS20695at 4170.715 kb on - strand, within ECOLIN_RS20695at 4170.715 kb on - strand, within ECOLIN_RS20695at 4170.810 kb on + strand, within ECOLIN_RS20695at 4170.815 kb on - strand, within ECOLIN_RS20695at 4171.118 kb on - strand, within ECOLIN_RS20695at 4171.201 kb on + strand, within ECOLIN_RS20695at 4171.227 kb on - strand, within ECOLIN_RS20695

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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4,168,489 + +2.4
4,169,040 - ECOLIN_RS20685 0.57 +4.8
4,169,040 - ECOLIN_RS20685 0.57 +2.5
4,169,126 + ECOLIN_RS20685 0.66 +2.8
4,169,140 - ECOLIN_RS20685 0.67 +2.8
4,169,150 - ECOLIN_RS20685 0.68 +2.1
4,169,219 - ECOLIN_RS20685 0.75 +2.7
4,169,219 - ECOLIN_RS20685 0.75 +3.1
4,169,223 + ECOLIN_RS20685 0.75 +2.5
4,169,311 + ECOLIN_RS20685 0.84 +3.2
4,169,312 - ECOLIN_RS20685 0.84 +2.6
4,169,456 - +3.3
4,169,733 - ECOLIN_RS20690 0.31 +2.4
4,169,794 + ECOLIN_RS20690 0.38 +2.4
4,169,796 + ECOLIN_RS20690 0.38 +1.1
4,169,860 + ECOLIN_RS20690 0.46 +0.8
4,169,861 - ECOLIN_RS20690 0.47 +3.9
4,169,867 - ECOLIN_RS20690 0.47 +3.1
4,170,010 - ECOLIN_RS20690 0.65 +2.7
4,170,013 + ECOLIN_RS20690 0.66 -0.1
4,170,013 + ECOLIN_RS20690 0.66 +2.7
4,170,013 + ECOLIN_RS20690 0.66 +1.6
4,170,014 - ECOLIN_RS20690 0.66 +3.1
4,170,014 - ECOLIN_RS20690 0.66 +2.3
4,170,014 - ECOLIN_RS20690 0.66 +2.3
4,170,014 - ECOLIN_RS20690 0.66 +2.8
4,170,108 - ECOLIN_RS20690 0.78 +2.4
4,170,275 - +2.8
4,170,332 - +3.6
4,170,333 + +2.5
4,170,334 - +4.2
4,170,479 + ECOLIN_RS20695 0.18 +1.9
4,170,481 + ECOLIN_RS20695 0.18 +1.8
4,170,482 - ECOLIN_RS20695 0.18 +3.4
4,170,546 + ECOLIN_RS20695 0.24 +2.1
4,170,603 + ECOLIN_RS20695 0.29 +2.0
4,170,709 + ECOLIN_RS20695 0.38 +1.7
4,170,709 + ECOLIN_RS20695 0.38 +3.2
4,170,711 + ECOLIN_RS20695 0.38 +2.8
4,170,711 + ECOLIN_RS20695 0.38 +4.3
4,170,711 + ECOLIN_RS20695 0.38 +2.3
4,170,711 + ECOLIN_RS20695 0.38 +1.8
4,170,711 + ECOLIN_RS20695 0.38 +2.4
4,170,712 - ECOLIN_RS20695 0.38 +3.6
4,170,712 - ECOLIN_RS20695 0.38 +1.8
4,170,712 - ECOLIN_RS20695 0.38 +2.2
4,170,712 - ECOLIN_RS20695 0.38 +2.9
4,170,714 + ECOLIN_RS20695 0.39 +2.7
4,170,715 - ECOLIN_RS20695 0.39 +1.7
4,170,715 - ECOLIN_RS20695 0.39 +3.0
4,170,810 + ECOLIN_RS20695 0.47 +3.2
4,170,815 - ECOLIN_RS20695 0.48 +3.6
4,171,118 - ECOLIN_RS20695 0.75 +3.0
4,171,201 + ECOLIN_RS20695 0.82 +3.8
4,171,227 - ECOLIN_RS20695 0.84 +2.7

Or see this region's nucleotide sequence