Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20670

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20665 and ECOLIN_RS20670 are separated by 81 nucleotidesECOLIN_RS20670 and ECOLIN_RS20675 are separated by 25 nucleotidesECOLIN_RS20675 and ECOLIN_RS20680 are separated by 9 nucleotides ECOLIN_RS20665: ECOLIN_RS20665 - glycosyltransferase family A protein, at 4,164,624 to 4,165,607 _RS20665 ECOLIN_RS20670: ECOLIN_RS20670 - UDP-galactose--(galactosyl) LPS alpha1,2-galactosyltransferase, at 4,165,689 to 4,166,717 _RS20670 ECOLIN_RS20675: ECOLIN_RS20675 - lipopolysaccharide core heptose(II) kinase RfaY, at 4,166,743 to 4,167,435 _RS20675 ECOLIN_RS20680: ECOLIN_RS20680 - glycosyltransferase, at 4,167,445 to 4,168,440 _RS20680 Position (kb) 4165 4166 4167Strain fitness (log2 ratio) -1 0 1 2 3at 4164.981 kb on - strand, within ECOLIN_RS20665at 4165.135 kb on - strand, within ECOLIN_RS20665at 4165.314 kb on + strand, within ECOLIN_RS20665at 4166.086 kb on + strand, within ECOLIN_RS20670at 4166.709 kb on + strandat 4167.194 kb on + strand, within ECOLIN_RS20675at 4167.422 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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4,164,981 - ECOLIN_RS20665 0.36 -1.2
4,165,135 - ECOLIN_RS20665 0.52 -0.6
4,165,314 + ECOLIN_RS20665 0.70 -1.3
4,166,086 + ECOLIN_RS20670 0.39 +1.3
4,166,709 + +0.9
4,167,194 + ECOLIN_RS20675 0.65 +3.5
4,167,422 - +1.2

Or see this region's nucleotide sequence