Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS14475

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS14470 and ECOLIN_RS14475 are separated by 257 nucleotidesECOLIN_RS14475 and ECOLIN_RS14480 overlap by 4 nucleotidesECOLIN_RS14480 and ECOLIN_RS14485 are separated by 57 nucleotides ECOLIN_RS14470: ECOLIN_RS14470 - phosphoribosylformylglycinamidine synthase, at 2,926,588 to 2,930,475 _RS14470 ECOLIN_RS14475: ECOLIN_RS14475 - membrane-bound lytic murein transglycosylase MltF, at 2,930,733 to 2,932,289 _RS14475 ECOLIN_RS14480: ECOLIN_RS14480 - tRNA adenosine(34) deaminase TadA, at 2,932,286 to 2,932,789 _RS14480 ECOLIN_RS14485: ECOLIN_RS14485 - phosphatidylglycerophosphatase C, at 2,932,847 to 2,933,482 _RS14485 Position (kb) 2930 2931 2932 2933Strain fitness (log2 ratio) -2 -1 0 1at 2930.138 kb on + strandat 2930.138 kb on + strandat 2930.475 kb on + strandat 2930.532 kb on - strandat 2931.064 kb on - strand, within ECOLIN_RS14475at 2931.327 kb on + strand, within ECOLIN_RS14475at 2931.449 kb on + strand, within ECOLIN_RS14475at 2931.552 kb on + strand, within ECOLIN_RS14475at 2931.604 kb on + strand, within ECOLIN_RS14475at 2931.624 kb on + strand, within ECOLIN_RS14475at 2931.624 kb on + strand, within ECOLIN_RS14475at 2931.882 kb on + strand, within ECOLIN_RS14475at 2932.807 kb on + strandat 2932.808 kb on - strandat 2932.808 kb on - strandat 2932.808 kb on - strandat 2932.823 kb on + strandat 2932.844 kb on - strandat 2933.164 kb on - strand, within ECOLIN_RS14485

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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2,930,138 + +0.2
2,930,138 + +0.5
2,930,475 + -2.2
2,930,532 - +0.4
2,931,064 - ECOLIN_RS14475 0.21 +0.7
2,931,327 + ECOLIN_RS14475 0.38 +1.3
2,931,449 + ECOLIN_RS14475 0.46 +1.3
2,931,552 + ECOLIN_RS14475 0.53 +1.1
2,931,604 + ECOLIN_RS14475 0.56 +1.2
2,931,624 + ECOLIN_RS14475 0.57 +1.6
2,931,624 + ECOLIN_RS14475 0.57 +0.9
2,931,882 + ECOLIN_RS14475 0.74 +1.2
2,932,807 + -2.4
2,932,808 - +1.0
2,932,808 - +0.2
2,932,808 - -0.8
2,932,823 + -2.5
2,932,844 - +0.1
2,933,164 - ECOLIN_RS14485 0.50 -0.3

Or see this region's nucleotide sequence