Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS14205

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS14200 and ECOLIN_RS14205 are separated by 4 nucleotidesECOLIN_RS14205 and ECOLIN_RS14210 are separated by 38 nucleotides ECOLIN_RS14200: ECOLIN_RS14200 - polyphosphate kinase 1, at 2,852,073 to 2,854,139 _RS14200 ECOLIN_RS14205: ECOLIN_RS14205 - exopolyphosphatase, at 2,854,144 to 2,855,685 _RS14205 ECOLIN_RS14210: ECOLIN_RS14210 - cyclic-guanylate-specific phosphodiesterase PdeF, at 2,855,724 to 2,857,967 _RS14210 Position (kb) 2854 2855 2856Strain fitness (log2 ratio) -1 0 1 2 3at 2853.577 kb on - strand, within ECOLIN_RS14200at 2853.580 kb on + strand, within ECOLIN_RS14200at 2854.850 kb on + strand, within ECOLIN_RS14205at 2855.303 kb on - strand, within ECOLIN_RS14205at 2855.335 kb on - strand, within ECOLIN_RS14205at 2856.276 kb on - strand, within ECOLIN_RS14210

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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2,853,577 - ECOLIN_RS14200 0.73 +2.8
2,853,580 + ECOLIN_RS14200 0.73 +2.7
2,854,850 + ECOLIN_RS14205 0.46 -0.8
2,855,303 - ECOLIN_RS14205 0.75 +0.8
2,855,335 - ECOLIN_RS14205 0.77 -1.1
2,856,276 - ECOLIN_RS14210 0.25 +0.1

Or see this region's nucleotide sequence