Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS11000

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS10985 and ECOLIN_RS10990 are separated by 24 nucleotidesECOLIN_RS10990 and ECOLIN_RS10995 overlap by 1 nucleotidesECOLIN_RS10995 and ECOLIN_RS11000 are separated by 77 nucleotidesECOLIN_RS11000 and ECOLIN_RS11005 are separated by 33 nucleotidesECOLIN_RS11005 and ECOLIN_RS11010 are separated by 186 nucleotides ECOLIN_RS10985: ECOLIN_RS10985 - flagellar filament capping protein FliD, at 2,132,391 to 2,133,797 _RS10985 ECOLIN_RS10990: ECOLIN_RS10990 - flagellar export chaperone FliS, at 2,133,822 to 2,134,232 _RS10990 ECOLIN_RS10995: ECOLIN_RS10995 - flagella biosynthesis regulatory protein FliT, at 2,134,232 to 2,134,597 _RS10995 ECOLIN_RS11000: ECOLIN_RS11000 - alpha-amylase, at 2,134,675 to 2,136,162 _RS11000 ECOLIN_RS11005: ECOLIN_RS11005 - lipoprotein YedD, at 2,136,196 to 2,136,609 _RS11005 ECOLIN_RS11010: ECOLIN_RS11010 - selenium metabolism membrane protein YedE/FdhT, at 2,136,796 to 2,138,001 _RS11010 Position (kb) 2134 2135 2136 2137Strain fitness (log2 ratio) -1 0 1at 2134.465 kb on - strand, within ECOLIN_RS10995at 2135.017 kb on - strand, within ECOLIN_RS11000at 2136.649 kb on - strandat 2136.747 kb on - strandat 2136.886 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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2,134,465 - ECOLIN_RS10995 0.64 -1.1
2,135,017 - ECOLIN_RS11000 0.23 -0.0
2,136,649 - +1.2
2,136,747 - -0.8
2,136,886 + -0.2

Or see this region's nucleotide sequence