Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS07160

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS07150 and ECOLIN_RS07155 are separated by 262 nucleotidesECOLIN_RS07155 and ECOLIN_RS07160 overlap by 1 nucleotidesECOLIN_RS07160 and ECOLIN_RS07165 are separated by 44 nucleotidesECOLIN_RS07165 and ECOLIN_RS07170 are separated by 145 nucleotides ECOLIN_RS07150: ECOLIN_RS07150 - hemolysin HlyE, at 1,393,430 to 1,393,753 _RS07150 ECOLIN_RS07155: ECOLIN_RS07155 - translesion error-prone DNA polymerase V autoproteolytic subunit, at 1,394,016 to 1,394,435 _RS07155 ECOLIN_RS07160: ECOLIN_RS07160 - DNA polymerase V catalytic protein, at 1,394,435 to 1,395,703 _RS07160 ECOLIN_RS07165: ECOLIN_RS07165 - disulfide bond formation protein DsbB, at 1,395,748 to 1,396,278 _RS07165 ECOLIN_RS07170: ECOLIN_RS07170 - Na(+)/H(+) antiporter NhaB, at 1,396,424 to 1,397,965 _RS07170 Position (kb) 1394 1395 1396Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1393.919 kb on + strandat 1395.781 kb on - strandat 1396.043 kb on + strand, within ECOLIN_RS07165at 1396.064 kb on - strand, within ECOLIN_RS07165

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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1,393,919 + +0.0
1,395,781 - -0.2
1,396,043 + ECOLIN_RS07165 0.56 -4.7
1,396,064 - ECOLIN_RS07165 0.60 -1.9

Or see this region's nucleotide sequence