Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS04350

Experiment: TP7

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS04345 and ECOLIN_RS04350 are separated by 271 nucleotidesECOLIN_RS04350 and ECOLIN_RS04355 are separated by 185 nucleotidesECOLIN_RS04355 and ECOLIN_RS04360 overlap by 4 nucleotides ECOLIN_RS04345: ECOLIN_RS04345 - phosphoethanolamine transferase, at 878,477 to 880,060 _RS04345 ECOLIN_RS04350: ECOLIN_RS04350 - manganase accumulation protein MntS, at 880,332 to 880,460 _RS04350 ECOLIN_RS04355: ECOLIN_RS04355 - manganese-binding transcriptional regulator MntR, at 880,646 to 881,113 _RS04355 ECOLIN_RS04360: ECOLIN_RS04360 - anion transporter, at 881,110 to 882,228 _RS04360 Position (kb) 880 881Strain fitness (log2 ratio) -1 0 1at 880.166 kb on - strandat 880.389 kb on - strand, within ECOLIN_RS04350at 880.389 kb on - strand, within ECOLIN_RS04350at 880.402 kb on - strand, within ECOLIN_RS04350at 880.408 kb on - strand, within ECOLIN_RS04350at 880.440 kb on - strand, within ECOLIN_RS04350at 880.511 kb on - strandat 880.541 kb on + strandat 880.615 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction TP7
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880,166 - +1.4
880,389 - ECOLIN_RS04350 0.44 +0.3
880,389 - ECOLIN_RS04350 0.44 +0.3
880,402 - ECOLIN_RS04350 0.54 +1.2
880,408 - ECOLIN_RS04350 0.59 -0.3
880,440 - ECOLIN_RS04350 0.84 +0.1
880,511 - +0.8
880,541 + +0.3
880,615 + +0.2

Or see this region's nucleotide sequence