Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS16165

Experiment: Bas04

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS16160 and ECOLIN_RS16165 are separated by 165 nucleotidesECOLIN_RS16165 and ECOLIN_RS16170 are separated by 117 nucleotidesECOLIN_RS16170 and ECOLIN_RS16175 are separated by 23 nucleotides ECOLIN_RS16160: ECOLIN_RS16160 - 6-phospho-beta-glucosidase BglA, at 3,276,153 to 3,277,586 _RS16160 ECOLIN_RS16165: ECOLIN_RS16165 - aminomethyl-transferring glycine dehydrogenase, at 3,277,752 to 3,280,625 _RS16165 ECOLIN_RS16170: ECOLIN_RS16170 - glycine cleavage system protein GcvH, at 3,280,743 to 3,281,132 _RS16170 ECOLIN_RS16175: ECOLIN_RS16175 - glycine cleavage system aminomethyltransferase GcvT, at 3,281,156 to 3,282,250 _RS16175 Position (kb) 3277 3278 3279 3280 3281Strain fitness (log2 ratio) -2 -1 0 1 2at 3276.780 kb on + strand, within ECOLIN_RS16160at 3276.780 kb on + strand, within ECOLIN_RS16160at 3276.780 kb on + strand, within ECOLIN_RS16160at 3276.781 kb on - strand, within ECOLIN_RS16160at 3276.866 kb on + strand, within ECOLIN_RS16160at 3276.866 kb on + strand, within ECOLIN_RS16160at 3276.894 kb on + strand, within ECOLIN_RS16160at 3276.894 kb on + strand, within ECOLIN_RS16160at 3276.990 kb on + strand, within ECOLIN_RS16160at 3276.990 kb on + strand, within ECOLIN_RS16160at 3277.022 kb on + strand, within ECOLIN_RS16160at 3277.054 kb on + strand, within ECOLIN_RS16160at 3277.096 kb on - strand, within ECOLIN_RS16160at 3277.242 kb on + strand, within ECOLIN_RS16160at 3277.478 kb on + strandat 3277.482 kb on + strandat 3277.541 kb on + strandat 3277.699 kb on + strandat 3277.731 kb on + strandat 3277.732 kb on - strandat 3278.254 kb on + strand, within ECOLIN_RS16165at 3278.254 kb on + strand, within ECOLIN_RS16165at 3278.255 kb on - strand, within ECOLIN_RS16165at 3278.255 kb on - strand, within ECOLIN_RS16165at 3278.255 kb on - strand, within ECOLIN_RS16165at 3278.255 kb on - strand, within ECOLIN_RS16165at 3278.343 kb on - strand, within ECOLIN_RS16165at 3278.682 kb on - strand, within ECOLIN_RS16165at 3278.786 kb on + strand, within ECOLIN_RS16165at 3279.154 kb on + strand, within ECOLIN_RS16165at 3279.380 kb on + strand, within ECOLIN_RS16165at 3279.583 kb on + strand, within ECOLIN_RS16165at 3279.621 kb on - strand, within ECOLIN_RS16165at 3279.621 kb on - strand, within ECOLIN_RS16165at 3279.726 kb on - strand, within ECOLIN_RS16165at 3279.748 kb on + strand, within ECOLIN_RS16165at 3279.749 kb on - strand, within ECOLIN_RS16165at 3279.862 kb on - strand, within ECOLIN_RS16165at 3280.301 kb on - strand, within ECOLIN_RS16165at 3280.326 kb on - strand, within ECOLIN_RS16165at 3280.326 kb on - strand, within ECOLIN_RS16165at 3280.375 kb on + strandat 3280.583 kb on + strandat 3280.612 kb on - strandat 3280.756 kb on - strandat 3280.756 kb on - strandat 3280.844 kb on - strand, within ECOLIN_RS16170at 3281.060 kb on - strand, within ECOLIN_RS16170at 3281.079 kb on - strand, within ECOLIN_RS16170at 3281.105 kb on - strandat 3281.122 kb on - strandat 3281.204 kb on - strandat 3281.252 kb on - strandat 3281.281 kb on + strand, within ECOLIN_RS16175at 3281.281 kb on + strand, within ECOLIN_RS16175at 3281.282 kb on - strand, within ECOLIN_RS16175at 3281.282 kb on - strand, within ECOLIN_RS16175at 3281.282 kb on - strand, within ECOLIN_RS16175at 3281.282 kb on - strand, within ECOLIN_RS16175at 3281.282 kb on - strand, within ECOLIN_RS16175at 3281.306 kb on - strand, within ECOLIN_RS16175at 3281.544 kb on + strand, within ECOLIN_RS16175

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas04
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3,276,780 + ECOLIN_RS16160 0.44 +0.2
3,276,780 + ECOLIN_RS16160 0.44 +0.1
3,276,780 + ECOLIN_RS16160 0.44 -0.3
3,276,781 - ECOLIN_RS16160 0.44 +0.6
3,276,866 + ECOLIN_RS16160 0.50 -0.5
3,276,866 + ECOLIN_RS16160 0.50 -0.3
3,276,894 + ECOLIN_RS16160 0.52 +0.2
3,276,894 + ECOLIN_RS16160 0.52 -0.4
3,276,990 + ECOLIN_RS16160 0.58 -0.3
3,276,990 + ECOLIN_RS16160 0.58 -1.2
3,277,022 + ECOLIN_RS16160 0.61 -0.1
3,277,054 + ECOLIN_RS16160 0.63 +0.1
3,277,096 - ECOLIN_RS16160 0.66 +1.0
3,277,242 + ECOLIN_RS16160 0.76 -1.5
3,277,478 + -0.1
3,277,482 + -0.2
3,277,541 + -1.1
3,277,699 + +0.2
3,277,731 + +0.5
3,277,732 - -0.2
3,278,254 + ECOLIN_RS16165 0.17 -0.0
3,278,254 + ECOLIN_RS16165 0.17 -0.5
3,278,255 - ECOLIN_RS16165 0.18 +1.1
3,278,255 - ECOLIN_RS16165 0.18 -0.1
3,278,255 - ECOLIN_RS16165 0.18 -2.3
3,278,255 - ECOLIN_RS16165 0.18 -0.5
3,278,343 - ECOLIN_RS16165 0.21 +2.0
3,278,682 - ECOLIN_RS16165 0.32 +0.9
3,278,786 + ECOLIN_RS16165 0.36 +0.3
3,279,154 + ECOLIN_RS16165 0.49 -0.3
3,279,380 + ECOLIN_RS16165 0.57 +0.0
3,279,583 + ECOLIN_RS16165 0.64 +0.4
3,279,621 - ECOLIN_RS16165 0.65 -0.8
3,279,621 - ECOLIN_RS16165 0.65 +2.1
3,279,726 - ECOLIN_RS16165 0.69 +0.3
3,279,748 + ECOLIN_RS16165 0.69 -0.7
3,279,749 - ECOLIN_RS16165 0.69 +0.0
3,279,862 - ECOLIN_RS16165 0.73 -0.1
3,280,301 - ECOLIN_RS16165 0.89 +0.2
3,280,326 - ECOLIN_RS16165 0.90 +0.3
3,280,326 - ECOLIN_RS16165 0.90 +0.4
3,280,375 + +0.6
3,280,583 + -0.5
3,280,612 - -0.8
3,280,756 - +0.3
3,280,756 - -0.4
3,280,844 - ECOLIN_RS16170 0.26 +0.1
3,281,060 - ECOLIN_RS16170 0.81 +0.1
3,281,079 - ECOLIN_RS16170 0.86 +0.2
3,281,105 - -0.1
3,281,122 - +0.6
3,281,204 - +0.1
3,281,252 - -0.0
3,281,281 + ECOLIN_RS16175 0.11 -0.4
3,281,281 + ECOLIN_RS16175 0.11 +0.9
3,281,282 - ECOLIN_RS16175 0.12 +0.1
3,281,282 - ECOLIN_RS16175 0.12 -0.0
3,281,282 - ECOLIN_RS16175 0.12 -0.3
3,281,282 - ECOLIN_RS16175 0.12 -0.2
3,281,282 - ECOLIN_RS16175 0.12 +0.1
3,281,306 - ECOLIN_RS16175 0.14 +0.3
3,281,544 + ECOLIN_RS16175 0.35 -0.3

Or see this region's nucleotide sequence