Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS24385

Experiment: Bas04

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS24375 and ECOLIN_RS24380 are separated by 6 nucleotidesECOLIN_RS24380 and ECOLIN_RS24385 are separated by 4 nucleotidesECOLIN_RS24385 and ECOLIN_RS24390 are separated by 41 nucleotidesECOLIN_RS24390 and ECOLIN_RS24395 are separated by 401 nucleotides ECOLIN_RS24375: ECOLIN_RS24375 - 30S ribosomal protein S6, at 4,898,046 to 4,898,441 _RS24375 ECOLIN_RS24380: ECOLIN_RS24380 - primosomal replication protein N, at 4,898,448 to 4,898,762 _RS24380 ECOLIN_RS24385: ECOLIN_RS24385 - 30S ribosomal protein S18, at 4,898,767 to 4,898,994 _RS24385 ECOLIN_RS24390: ECOLIN_RS24390 - 50S ribosomal protein L9, at 4,899,036 to 4,899,485 _RS24390 ECOLIN_RS24395: ECOLIN_RS24395 - helix-turn-helix transcriptional regulator, at 4,899,887 to 4,900,378 _RS24395 Position (kb) 4898 4899Strain fitness (log2 ratio) -2 -1 0 1at 4897.883 kb on + strandat 4897.884 kb on - strandat 4899.013 kb on + strandat 4899.013 kb on + strandat 4899.013 kb on + strandat 4899.044 kb on + strandat 4899.056 kb on + strandat 4899.061 kb on + strandat 4899.061 kb on + strandat 4899.091 kb on + strand, within ECOLIN_RS24390at 4899.203 kb on + strand, within ECOLIN_RS24390at 4899.472 kb on + strandat 4899.472 kb on + strandat 4899.472 kb on + strandat 4899.505 kb on + strandat 4899.730 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas04
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4,897,883 + +0.1
4,897,884 - +0.6
4,899,013 + +0.1
4,899,013 + -0.3
4,899,013 + -0.3
4,899,044 + -0.7
4,899,056 + -2.7
4,899,061 + -2.3
4,899,061 + -2.0
4,899,091 + ECOLIN_RS24390 0.12 -1.8
4,899,203 + ECOLIN_RS24390 0.37 -1.5
4,899,472 + -1.3
4,899,472 + -1.3
4,899,472 + -2.1
4,899,505 + +0.2
4,899,730 - -0.2

Or see this region's nucleotide sequence