Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20580

Experiment: Bas04

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20570 and ECOLIN_RS20575 overlap by 1 nucleotidesECOLIN_RS20575 and ECOLIN_RS20580 overlap by 4 nucleotidesECOLIN_RS20580 and ECOLIN_RS20585 are separated by 47 nucleotidesECOLIN_RS20585 and ECOLIN_RS20590 are separated by 54 nucleotides ECOLIN_RS20570: ECOLIN_RS20570 - L-lactate permease, at 4,145,065 to 4,146,720 _RS20570 ECOLIN_RS20575: ECOLIN_RS20575 - transcriptional regulator LldR, at 4,146,720 to 4,147,496 _RS20575 ECOLIN_RS20580: ECOLIN_RS20580 - quinone-dependent L-lactate dehydrogenase, at 4,147,493 to 4,148,683 _RS20580 ECOLIN_RS20585: ECOLIN_RS20585 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 4,148,731 to 4,149,204 _RS20585 ECOLIN_RS20590: ECOLIN_RS20590 - serine O-acetyltransferase, at 4,149,259 to 4,150,080 _RS20590 Position (kb) 4147 4148 4149Strain fitness (log2 ratio) -1 0 1at 4146.701 kb on + strandat 4146.886 kb on + strand, within ECOLIN_RS20575at 4147.197 kb on + strand, within ECOLIN_RS20575at 4147.197 kb on - strand, within ECOLIN_RS20575at 4147.197 kb on - strand, within ECOLIN_RS20575at 4147.197 kb on - strand, within ECOLIN_RS20575at 4147.498 kb on + strandat 4147.564 kb on + strandat 4147.565 kb on - strandat 4147.590 kb on - strandat 4147.665 kb on + strand, within ECOLIN_RS20580at 4147.665 kb on + strand, within ECOLIN_RS20580at 4147.666 kb on - strand, within ECOLIN_RS20580at 4147.742 kb on + strand, within ECOLIN_RS20580at 4147.742 kb on + strand, within ECOLIN_RS20580at 4147.742 kb on + strand, within ECOLIN_RS20580at 4147.742 kb on + strand, within ECOLIN_RS20580at 4147.743 kb on - strand, within ECOLIN_RS20580at 4147.743 kb on - strand, within ECOLIN_RS20580at 4147.743 kb on - strand, within ECOLIN_RS20580at 4147.877 kb on + strand, within ECOLIN_RS20580at 4148.107 kb on + strand, within ECOLIN_RS20580at 4148.150 kb on + strand, within ECOLIN_RS20580at 4148.150 kb on + strand, within ECOLIN_RS20580at 4148.150 kb on + strand, within ECOLIN_RS20580at 4148.150 kb on + strand, within ECOLIN_RS20580at 4148.331 kb on + strand, within ECOLIN_RS20580at 4148.341 kb on - strand, within ECOLIN_RS20580at 4148.419 kb on - strand, within ECOLIN_RS20580at 4148.443 kb on + strand, within ECOLIN_RS20580at 4148.443 kb on + strand, within ECOLIN_RS20580at 4148.528 kb on - strand, within ECOLIN_RS20580at 4148.528 kb on - strand, within ECOLIN_RS20580at 4148.696 kb on + strandat 4148.730 kb on + strandat 4148.730 kb on + strandat 4148.735 kb on + strandat 4148.735 kb on + strandat 4148.745 kb on - strandat 4148.772 kb on + strandat 4148.773 kb on - strandat 4148.976 kb on + strand, within ECOLIN_RS20585at 4148.996 kb on + strand, within ECOLIN_RS20585at 4149.154 kb on + strand, within ECOLIN_RS20585at 4149.275 kb on + strandat 4149.286 kb on - strandat 4149.315 kb on + strandat 4149.559 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.650 kb on - strand, within ECOLIN_RS20590at 4149.650 kb on - strand, within ECOLIN_RS20590

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas04
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4,146,701 + -0.1
4,146,886 + ECOLIN_RS20575 0.21 -0.1
4,147,197 + ECOLIN_RS20575 0.61 -0.3
4,147,197 - ECOLIN_RS20575 0.61 +0.4
4,147,197 - ECOLIN_RS20575 0.61 +0.3
4,147,197 - ECOLIN_RS20575 0.61 +0.3
4,147,498 + +0.1
4,147,564 + +0.1
4,147,565 - -0.6
4,147,590 - +0.1
4,147,665 + ECOLIN_RS20580 0.14 +0.6
4,147,665 + ECOLIN_RS20580 0.14 -0.0
4,147,666 - ECOLIN_RS20580 0.15 +0.9
4,147,742 + ECOLIN_RS20580 0.21 -0.4
4,147,742 + ECOLIN_RS20580 0.21 +0.0
4,147,742 + ECOLIN_RS20580 0.21 +0.2
4,147,742 + ECOLIN_RS20580 0.21 -0.0
4,147,743 - ECOLIN_RS20580 0.21 +0.4
4,147,743 - ECOLIN_RS20580 0.21 -0.5
4,147,743 - ECOLIN_RS20580 0.21 -0.6
4,147,877 + ECOLIN_RS20580 0.32 +0.3
4,148,107 + ECOLIN_RS20580 0.52 +0.1
4,148,150 + ECOLIN_RS20580 0.55 +0.0
4,148,150 + ECOLIN_RS20580 0.55 +0.7
4,148,150 + ECOLIN_RS20580 0.55 +0.3
4,148,150 + ECOLIN_RS20580 0.55 +0.2
4,148,331 + ECOLIN_RS20580 0.70 +0.2
4,148,341 - ECOLIN_RS20580 0.71 -0.9
4,148,419 - ECOLIN_RS20580 0.78 +0.1
4,148,443 + ECOLIN_RS20580 0.80 +0.3
4,148,443 + ECOLIN_RS20580 0.80 +0.1
4,148,528 - ECOLIN_RS20580 0.87 -0.5
4,148,528 - ECOLIN_RS20580 0.87 +0.3
4,148,696 + +1.6
4,148,730 + +0.1
4,148,730 + -0.9
4,148,735 + +0.6
4,148,735 + +0.0
4,148,745 - -0.9
4,148,772 + -0.5
4,148,773 - +0.0
4,148,976 + ECOLIN_RS20585 0.52 -0.1
4,148,996 + ECOLIN_RS20585 0.56 +0.2
4,149,154 + ECOLIN_RS20585 0.89 +0.5
4,149,275 + +0.6
4,149,286 - +0.1
4,149,315 + -0.0
4,149,559 + ECOLIN_RS20590 0.36 -0.2
4,149,649 + ECOLIN_RS20590 0.47 -0.9
4,149,649 + ECOLIN_RS20590 0.47 -0.2
4,149,649 + ECOLIN_RS20590 0.47 +1.5
4,149,649 + ECOLIN_RS20590 0.47 -0.9
4,149,650 - ECOLIN_RS20590 0.48 -0.3
4,149,650 - ECOLIN_RS20590 0.48 -0.9

Or see this region's nucleotide sequence