Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20130

Experiment: Bas04

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20125 and ECOLIN_RS20130 are separated by 404 nucleotidesECOLIN_RS20130 and ECOLIN_RS20135 are separated by 50 nucleotides ECOLIN_RS20125: ECOLIN_RS20125 - cytochrome c peroxidase, at 4,039,868 to 4,041,265 _RS20125 ECOLIN_RS20130: ECOLIN_RS20130 - alpha,alpha-trehalase, at 4,041,670 to 4,043,319 _RS20130 ECOLIN_RS20135: ECOLIN_RS20135 - response regulator transcription factor, at 4,043,370 to 4,043,972 _RS20135 Position (kb) 4041 4042 4043 4044Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 4040.777 kb on - strand, within ECOLIN_RS20125at 4040.790 kb on + strand, within ECOLIN_RS20125at 4040.902 kb on - strand, within ECOLIN_RS20125at 4040.997 kb on + strand, within ECOLIN_RS20125at 4041.009 kb on - strand, within ECOLIN_RS20125at 4041.043 kb on + strand, within ECOLIN_RS20125at 4041.045 kb on + strand, within ECOLIN_RS20125at 4041.048 kb on + strand, within ECOLIN_RS20125at 4041.051 kb on + strand, within ECOLIN_RS20125at 4041.052 kb on - strand, within ECOLIN_RS20125at 4041.059 kb on + strand, within ECOLIN_RS20125at 4041.124 kb on + strand, within ECOLIN_RS20125at 4041.172 kb on + strandat 4041.192 kb on + strandat 4041.192 kb on + strandat 4041.247 kb on + strandat 4041.247 kb on + strandat 4041.248 kb on - strandat 4041.539 kb on - strandat 4041.670 kb on - strandat 4041.936 kb on + strand, within ECOLIN_RS20130at 4042.036 kb on + strand, within ECOLIN_RS20130at 4042.040 kb on + strand, within ECOLIN_RS20130at 4042.040 kb on + strand, within ECOLIN_RS20130at 4042.153 kb on + strand, within ECOLIN_RS20130at 4042.153 kb on + strand, within ECOLIN_RS20130at 4042.153 kb on + strand, within ECOLIN_RS20130at 4042.153 kb on + strand, within ECOLIN_RS20130at 4042.153 kb on + strand, within ECOLIN_RS20130at 4042.153 kb on + strand, within ECOLIN_RS20130at 4042.153 kb on + strand, within ECOLIN_RS20130at 4042.153 kb on + strand, within ECOLIN_RS20130at 4042.154 kb on - strand, within ECOLIN_RS20130at 4042.154 kb on - strand, within ECOLIN_RS20130at 4042.154 kb on - strand, within ECOLIN_RS20130at 4042.155 kb on + strand, within ECOLIN_RS20130at 4042.156 kb on - strand, within ECOLIN_RS20130at 4042.156 kb on - strand, within ECOLIN_RS20130at 4042.201 kb on + strand, within ECOLIN_RS20130at 4042.319 kb on - strand, within ECOLIN_RS20130at 4042.426 kb on + strand, within ECOLIN_RS20130at 4042.426 kb on + strand, within ECOLIN_RS20130at 4042.936 kb on + strand, within ECOLIN_RS20130at 4042.937 kb on - strand, within ECOLIN_RS20130at 4043.100 kb on + strand, within ECOLIN_RS20130at 4043.170 kb on + strandat 4043.171 kb on - strandat 4043.171 kb on - strandat 4043.317 kb on + strandat 4043.318 kb on - strandat 4043.382 kb on - strandat 4043.385 kb on + strandat 4043.472 kb on - strand, within ECOLIN_RS20135at 4043.495 kb on + strand, within ECOLIN_RS20135at 4043.496 kb on - strand, within ECOLIN_RS20135at 4043.524 kb on - strand, within ECOLIN_RS20135at 4043.564 kb on + strand, within ECOLIN_RS20135at 4043.565 kb on - strand, within ECOLIN_RS20135at 4043.628 kb on - strand, within ECOLIN_RS20135at 4043.628 kb on - strand, within ECOLIN_RS20135at 4043.628 kb on - strand, within ECOLIN_RS20135at 4043.628 kb on - strand, within ECOLIN_RS20135at 4043.628 kb on - strand, within ECOLIN_RS20135at 4043.688 kb on - strand, within ECOLIN_RS20135at 4043.697 kb on - strand, within ECOLIN_RS20135at 4043.697 kb on - strand, within ECOLIN_RS20135at 4043.735 kb on + strand, within ECOLIN_RS20135at 4043.903 kb on + strand, within ECOLIN_RS20135at 4044.022 kb on + strandat 4044.176 kb on + strandat 4044.269 kb on + strandat 4044.277 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas04
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4,040,777 - ECOLIN_RS20125 0.65 -0.4
4,040,790 + ECOLIN_RS20125 0.66 -0.1
4,040,902 - ECOLIN_RS20125 0.74 +0.1
4,040,997 + ECOLIN_RS20125 0.81 -0.0
4,041,009 - ECOLIN_RS20125 0.82 -0.3
4,041,043 + ECOLIN_RS20125 0.84 +0.1
4,041,045 + ECOLIN_RS20125 0.84 -1.4
4,041,048 + ECOLIN_RS20125 0.84 +0.0
4,041,051 + ECOLIN_RS20125 0.85 -0.2
4,041,052 - ECOLIN_RS20125 0.85 -0.1
4,041,059 + ECOLIN_RS20125 0.85 -0.1
4,041,124 + ECOLIN_RS20125 0.90 +0.2
4,041,172 + +0.2
4,041,192 + -1.3
4,041,192 + -2.0
4,041,247 + +0.3
4,041,247 + +0.4
4,041,248 - -0.1
4,041,539 - +4.2
4,041,670 - +0.8
4,041,936 + ECOLIN_RS20130 0.16 -0.5
4,042,036 + ECOLIN_RS20130 0.22 +0.0
4,042,040 + ECOLIN_RS20130 0.22 +0.3
4,042,040 + ECOLIN_RS20130 0.22 -0.1
4,042,153 + ECOLIN_RS20130 0.29 +0.1
4,042,153 + ECOLIN_RS20130 0.29 -0.4
4,042,153 + ECOLIN_RS20130 0.29 -0.5
4,042,153 + ECOLIN_RS20130 0.29 -0.0
4,042,153 + ECOLIN_RS20130 0.29 +0.7
4,042,153 + ECOLIN_RS20130 0.29 -0.3
4,042,153 + ECOLIN_RS20130 0.29 +0.0
4,042,153 + ECOLIN_RS20130 0.29 +0.3
4,042,154 - ECOLIN_RS20130 0.29 +0.3
4,042,154 - ECOLIN_RS20130 0.29 -0.1
4,042,154 - ECOLIN_RS20130 0.29 +0.6
4,042,155 + ECOLIN_RS20130 0.29 +2.2
4,042,156 - ECOLIN_RS20130 0.29 +0.2
4,042,156 - ECOLIN_RS20130 0.29 +0.0
4,042,201 + ECOLIN_RS20130 0.32 +0.4
4,042,319 - ECOLIN_RS20130 0.39 +0.3
4,042,426 + ECOLIN_RS20130 0.46 +0.5
4,042,426 + ECOLIN_RS20130 0.46 -0.4
4,042,936 + ECOLIN_RS20130 0.77 +0.1
4,042,937 - ECOLIN_RS20130 0.77 -0.1
4,043,100 + ECOLIN_RS20130 0.87 +0.4
4,043,170 + +0.1
4,043,171 - -0.1
4,043,171 - -0.2
4,043,317 + -0.3
4,043,318 - -0.0
4,043,382 - +0.5
4,043,385 + -0.0
4,043,472 - ECOLIN_RS20135 0.17 +0.1
4,043,495 + ECOLIN_RS20135 0.21 +0.2
4,043,496 - ECOLIN_RS20135 0.21 -0.1
4,043,524 - ECOLIN_RS20135 0.26 +0.1
4,043,564 + ECOLIN_RS20135 0.32 -0.2
4,043,565 - ECOLIN_RS20135 0.32 +0.2
4,043,628 - ECOLIN_RS20135 0.43 +0.5
4,043,628 - ECOLIN_RS20135 0.43 +0.1
4,043,628 - ECOLIN_RS20135 0.43 +0.2
4,043,628 - ECOLIN_RS20135 0.43 +1.2
4,043,628 - ECOLIN_RS20135 0.43 +0.3
4,043,688 - ECOLIN_RS20135 0.53 +0.3
4,043,697 - ECOLIN_RS20135 0.54 +0.1
4,043,697 - ECOLIN_RS20135 0.54 -0.8
4,043,735 + ECOLIN_RS20135 0.61 -0.5
4,043,903 + ECOLIN_RS20135 0.88 +1.7
4,044,022 + +0.3
4,044,176 + +0.1
4,044,269 + +1.2
4,044,277 - +0.2

Or see this region's nucleotide sequence