Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS17015

Experiment: Bas04

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS17010 and ECOLIN_RS17015 are separated by 9 nucleotidesECOLIN_RS17015 and ECOLIN_RS17020 overlap by 4 nucleotides ECOLIN_RS17010: ECOLIN_RS17010 - polysaccharide biosynthesis/export family protein, at 3,422,998 to 3,424,674 _RS17010 ECOLIN_RS17015: ECOLIN_RS17015 - 3-deoxy-manno-octulosonate cytidylyltransferase, at 3,424,684 to 3,425,424 _RS17015 ECOLIN_RS17020: ECOLIN_RS17020 - capsular polysaccharide biosynthesis protein, at 3,425,421 to 3,427,448 _RS17020 Position (kb) 3424 3425 3426Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 3423.747 kb on + strand, within ECOLIN_RS17010at 3424.617 kb on + strandat 3424.786 kb on + strand, within ECOLIN_RS17015at 3424.960 kb on + strand, within ECOLIN_RS17015at 3424.960 kb on + strand, within ECOLIN_RS17015at 3424.965 kb on + strand, within ECOLIN_RS17015at 3425.018 kb on + strand, within ECOLIN_RS17015at 3425.212 kb on + strand, within ECOLIN_RS17015at 3425.294 kb on + strand, within ECOLIN_RS17015at 3425.895 kb on - strand, within ECOLIN_RS17020at 3426.408 kb on + strand, within ECOLIN_RS17020

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas04
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3,423,747 + ECOLIN_RS17010 0.45 -4.3
3,424,617 + +0.1
3,424,786 + ECOLIN_RS17015 0.14 -0.7
3,424,960 + ECOLIN_RS17015 0.37 -0.5
3,424,960 + ECOLIN_RS17015 0.37 -0.3
3,424,965 + ECOLIN_RS17015 0.38 +1.1
3,425,018 + ECOLIN_RS17015 0.45 -0.1
3,425,212 + ECOLIN_RS17015 0.71 +0.4
3,425,294 + ECOLIN_RS17015 0.82 +0.5
3,425,895 - ECOLIN_RS17020 0.23 -2.5
3,426,408 + ECOLIN_RS17020 0.49 -5.6

Or see this region's nucleotide sequence