Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU3049 and DVU3050 are separated by 123 nucleotides DVU3050 and mutT are separated by 221 nucleotides mutT and DVU3052 overlap by 1 nucleotides
DVU3049: DVU3049 - hemerythrin family protein (TIGR), at 3,175,305 to 3,175,718
DVU3049
DVU3050: DVU3050 - membrane protein, putative (TIGR), at 3,175,842 to 3,176,276
DVU3050
DVU3051: mutT - mutator mutT protein (TIGR), at 3,176,498 to 3,176,887
mutT
DVU3052: DVU3052 - ABC transporter, ATP-binding protein (TIGR), at 3,176,887 to 3,178,866
DVU3052
Position (kb)
3176
3177 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3175.522 kb on + strand, within DVU3049 at 3175.522 kb on + strand, within DVU3049 at 3175.530 kb on - strand, within DVU3049 at 3175.619 kb on - strand, within DVU3049 at 3175.744 kb on + strand at 3175.794 kb on - strand at 3175.794 kb on - strand at 3175.831 kb on + strand at 3175.839 kb on - strand at 3175.899 kb on - strand, within DVU3050 at 3175.924 kb on - strand, within DVU3050 at 3175.980 kb on - strand, within DVU3050 at 3175.995 kb on - strand, within DVU3050 at 3176.234 kb on - strand at 3176.425 kb on - strand at 3176.459 kb on - strand at 3176.466 kb on - strand at 3176.494 kb on - strand at 3176.588 kb on + strand, within mutT at 3176.596 kb on - strand, within mutT at 3176.617 kb on + strand, within mutT at 3176.713 kb on + strand, within mutT at 3176.731 kb on + strand, within mutT at 3176.764 kb on - strand, within mutT at 3176.813 kb on + strand, within mutT at 3176.828 kb on - strand, within mutT at 3176.897 kb on + strand at 3176.923 kb on + strand at 3176.951 kb on - strand at 3176.981 kb on - strand at 3177.021 kb on + strand at 3177.068 kb on + strand at 3177.076 kb on - strand at 3177.076 kb on - strand at 3177.112 kb on - strand, within DVU3052 at 3177.115 kb on + strand, within DVU3052 at 3177.179 kb on + strand, within DVU3052 at 3177.179 kb on + strand, within DVU3052 at 3177.218 kb on + strand, within DVU3052 at 3177.291 kb on - strand, within DVU3052 at 3177.410 kb on - strand, within DVU3052 at 3177.415 kb on - strand, within DVU3052 at 3177.593 kb on - strand, within DVU3052 at 3177.624 kb on + strand, within DVU3052 at 3177.647 kb on - strand, within DVU3052 at 3177.651 kb on + strand, within DVU3052 at 3177.651 kb on + strand, within DVU3052 at 3177.660 kb on + strand, within DVU3052 at 3177.668 kb on - strand, within DVU3052 at 3177.680 kb on + strand, within DVU3052 at 3177.764 kb on + strand, within DVU3052
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 3,175,522 + DVU3049 0.52 +0.5 3,175,522 + DVU3049 0.52 -0.2 3,175,530 - DVU3049 0.54 +0.1 3,175,619 - DVU3049 0.76 +0.3 3,175,744 + -0.9 3,175,794 - -0.6 3,175,794 - +0.6 3,175,831 + -0.4 3,175,839 - -0.3 3,175,899 - DVU3050 0.13 +1.0 3,175,924 - DVU3050 0.19 +0.0 3,175,980 - DVU3050 0.32 -0.6 3,175,995 - DVU3050 0.35 -0.5 3,176,234 - -0.7 3,176,425 - +0.1 3,176,459 - +0.9 3,176,466 - -0.3 3,176,494 - -0.3 3,176,588 + mutT DVU3051 0.23 -0.3 3,176,596 - mutT DVU3051 0.25 -1.4 3,176,617 + mutT DVU3051 0.31 +0.0 3,176,713 + mutT DVU3051 0.55 -0.5 3,176,731 + mutT DVU3051 0.60 -0.7 3,176,764 - mutT DVU3051 0.68 -2.4 3,176,813 + mutT DVU3051 0.81 -0.8 3,176,828 - mutT DVU3051 0.85 -0.2 3,176,897 + +0.5 3,176,923 + -0.3 3,176,951 - +2.1 3,176,981 - +0.1 3,177,021 + +0.2 3,177,068 + +0.4 3,177,076 - -0.0 3,177,076 - -0.6 3,177,112 - DVU3052 0.11 -0.1 3,177,115 + DVU3052 0.12 -0.3 3,177,179 + DVU3052 0.15 -1.3 3,177,179 + DVU3052 0.15 -0.2 3,177,218 + DVU3052 0.17 +1.6 3,177,291 - DVU3052 0.20 +0.1 3,177,410 - DVU3052 0.26 -1.2 3,177,415 - DVU3052 0.27 -0.8 3,177,593 - DVU3052 0.36 +1.0 3,177,624 + DVU3052 0.37 -0.2 3,177,647 - DVU3052 0.38 -0.5 3,177,651 + DVU3052 0.39 -1.1 3,177,651 + DVU3052 0.39 +0.8 3,177,660 + DVU3052 0.39 -0.5 3,177,668 - DVU3052 0.39 +0.1 3,177,680 + DVU3052 0.40 -0.7 3,177,764 + DVU3052 0.44 -0.1
Or see this region's nucleotide sequence