Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aroC and recD are separated by 172 nucleotides recD and DVU0896 are separated by 9 nucleotides
DVU0894: aroC - chorismate synthase (TIGR), at 984,956 to 986,020
aroC
DVU0895: recD - helicase, RecD/TraA family (TIGR), at 986,193 to 988,421
recD
DVU0896: DVU0896 - lipoprotein, NLP/P60 family (TIGR), at 988,431 to 989,825
DVU0896
Position (kb) 986
987
988
989 Strain fitness (log2 ratio) -2
-1
0
1 at 985.253 kb on + strand, within aroC at 985.704 kb on - strand, within aroC at 986.055 kb on + strand at 986.063 kb on - strand at 986.063 kb on - strand at 986.063 kb on - strand at 986.063 kb on - strand at 986.067 kb on + strand at 986.067 kb on + strand at 986.075 kb on - strand at 986.082 kb on + strand at 986.117 kb on + strand at 986.134 kb on + strand at 986.160 kb on + strand at 986.196 kb on - strand at 986.218 kb on + strand at 986.302 kb on - strand at 986.302 kb on - strand at 986.304 kb on - strand at 986.338 kb on - strand at 986.417 kb on + strand, within recD at 986.458 kb on - strand, within recD at 986.466 kb on - strand, within recD at 986.508 kb on - strand, within recD at 986.510 kb on + strand, within recD at 986.518 kb on - strand, within recD at 986.518 kb on - strand, within recD at 986.579 kb on + strand, within recD at 986.757 kb on - strand, within recD at 986.861 kb on + strand, within recD at 986.890 kb on - strand, within recD at 986.902 kb on - strand, within recD at 986.945 kb on - strand, within recD at 986.955 kb on - strand, within recD at 987.026 kb on + strand, within recD at 987.026 kb on + strand, within recD at 987.073 kb on - strand, within recD at 987.105 kb on + strand, within recD at 987.136 kb on - strand, within recD at 987.142 kb on - strand, within recD at 987.142 kb on - strand, within recD at 987.173 kb on + strand, within recD at 987.180 kb on + strand, within recD at 987.252 kb on + strand, within recD at 987.374 kb on + strand, within recD at 987.433 kb on + strand, within recD at 987.479 kb on - strand, within recD at 987.504 kb on + strand, within recD at 987.512 kb on + strand, within recD at 987.567 kb on - strand, within recD at 987.572 kb on + strand, within recD at 987.618 kb on + strand, within recD at 987.871 kb on - strand, within recD at 987.945 kb on - strand, within recD at 987.948 kb on + strand, within recD at 988.052 kb on - strand, within recD at 988.121 kb on - strand, within recD at 988.177 kb on - strand, within recD at 988.194 kb on + strand, within recD at 988.195 kb on + strand, within recD at 988.203 kb on - strand at 988.203 kb on - strand at 988.243 kb on - strand at 988.291 kb on - strand at 988.348 kb on - strand at 988.381 kb on + strand at 988.381 kb on + strand at 988.381 kb on + strand at 988.430 kb on + strand at 988.523 kb on + strand at 988.559 kb on - strand at 988.560 kb on + strand at 988.601 kb on - strand, within DVU0896 at 988.654 kb on - strand, within DVU0896 at 988.659 kb on - strand, within DVU0896 at 988.659 kb on - strand, within DVU0896 at 988.664 kb on - strand, within DVU0896 at 988.764 kb on - strand, within DVU0896 at 988.847 kb on + strand, within DVU0896 at 988.847 kb on + strand, within DVU0896 at 988.847 kb on + strand, within DVU0896 at 988.848 kb on - strand, within DVU0896 at 988.855 kb on - strand, within DVU0896 at 988.855 kb on - strand, within DVU0896 at 988.932 kb on + strand, within DVU0896 at 988.932 kb on + strand, within DVU0896 at 988.952 kb on + strand, within DVU0896 at 988.953 kb on + strand, within DVU0896 at 988.959 kb on + strand, within DVU0896 at 988.960 kb on - strand, within DVU0896 at 988.961 kb on - strand, within DVU0896 at 989.021 kb on - strand, within DVU0896 at 989.032 kb on + strand, within DVU0896 at 989.040 kb on - strand, within DVU0896 at 989.142 kb on - strand, within DVU0896 at 989.153 kb on + strand, within DVU0896 at 989.173 kb on - strand, within DVU0896 at 989.212 kb on - strand, within DVU0896 at 989.253 kb on + strand, within DVU0896 at 989.256 kb on - strand, within DVU0896 at 989.261 kb on - strand, within DVU0896 at 989.274 kb on + strand, within DVU0896 at 989.295 kb on - strand, within DVU0896 at 989.390 kb on + strand, within DVU0896 at 989.390 kb on + strand, within DVU0896
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 985,253 + aroC DVU0894 0.28 -1.3 985,704 - aroC DVU0894 0.70 +0.1 986,055 + +0.3 986,063 - +0.4 986,063 - +1.4 986,063 - +0.1 986,063 - -1.0 986,067 + -0.0 986,067 + +0.3 986,075 - -0.3 986,082 + +0.0 986,117 + +0.1 986,134 + +0.2 986,160 + +0.5 986,196 - -1.1 986,218 + -0.9 986,302 - -0.5 986,302 - -0.6 986,304 - +0.3 986,338 - -2.1 986,417 + recD DVU0895 0.10 -2.0 986,458 - recD DVU0895 0.12 +0.3 986,466 - recD DVU0895 0.12 -1.2 986,508 - recD DVU0895 0.14 -0.4 986,510 + recD DVU0895 0.14 +0.3 986,518 - recD DVU0895 0.15 -1.5 986,518 - recD DVU0895 0.15 -0.6 986,579 + recD DVU0895 0.17 -0.5 986,757 - recD DVU0895 0.25 -0.6 986,861 + recD DVU0895 0.30 -0.2 986,890 - recD DVU0895 0.31 -1.8 986,902 - recD DVU0895 0.32 -1.8 986,945 - recD DVU0895 0.34 -0.2 986,955 - recD DVU0895 0.34 -1.4 987,026 + recD DVU0895 0.37 -0.8 987,026 + recD DVU0895 0.37 -1.0 987,073 - recD DVU0895 0.39 -2.0 987,105 + recD DVU0895 0.41 -0.8 987,136 - recD DVU0895 0.42 -0.9 987,142 - recD DVU0895 0.43 +0.1 987,142 - recD DVU0895 0.43 +1.1 987,173 + recD DVU0895 0.44 -0.3 987,180 + recD DVU0895 0.44 -0.9 987,252 + recD DVU0895 0.48 -1.0 987,374 + recD DVU0895 0.53 +0.3 987,433 + recD DVU0895 0.56 -0.5 987,479 - recD DVU0895 0.58 -0.6 987,504 + recD DVU0895 0.59 +0.4 987,512 + recD DVU0895 0.59 +0.6 987,567 - recD DVU0895 0.62 -0.9 987,572 + recD DVU0895 0.62 -0.9 987,618 + recD DVU0895 0.64 -0.8 987,871 - recD DVU0895 0.75 -0.6 987,945 - recD DVU0895 0.79 -0.8 987,948 + recD DVU0895 0.79 -1.7 988,052 - recD DVU0895 0.83 +1.3 988,121 - recD DVU0895 0.86 -0.6 988,177 - recD DVU0895 0.89 -0.8 988,194 + recD DVU0895 0.90 +0.4 988,195 + recD DVU0895 0.90 -1.6 988,203 - -2.3 988,203 - +1.1 988,243 - +0.3 988,291 - +0.7 988,348 - +0.3 988,381 + -0.1 988,381 + -1.1 988,381 + -0.3 988,430 + +0.1 988,523 + +0.1 988,559 - -0.2 988,560 + +0.0 988,601 - DVU0896 0.12 +0.2 988,654 - DVU0896 0.16 -0.2 988,659 - DVU0896 0.16 -0.2 988,659 - DVU0896 0.16 -0.5 988,664 - DVU0896 0.17 -0.1 988,764 - DVU0896 0.24 -0.6 988,847 + DVU0896 0.30 -0.5 988,847 + DVU0896 0.30 -1.7 988,847 + DVU0896 0.30 +0.6 988,848 - DVU0896 0.30 +0.0 988,855 - DVU0896 0.30 -0.7 988,855 - DVU0896 0.30 -0.7 988,932 + DVU0896 0.36 -0.2 988,932 + DVU0896 0.36 -0.0 988,952 + DVU0896 0.37 +0.1 988,953 + DVU0896 0.37 -0.6 988,959 + DVU0896 0.38 -0.0 988,960 - DVU0896 0.38 -0.5 988,961 - DVU0896 0.38 +0.2 989,021 - DVU0896 0.42 -0.0 989,032 + DVU0896 0.43 +0.1 989,040 - DVU0896 0.44 -0.3 989,142 - DVU0896 0.51 -0.3 989,153 + DVU0896 0.52 -0.4 989,173 - DVU0896 0.53 -2.2 989,212 - DVU0896 0.56 +0.3 989,253 + DVU0896 0.59 -1.2 989,256 - DVU0896 0.59 +0.0 989,261 - DVU0896 0.59 -0.2 989,274 + DVU0896 0.60 -0.6 989,295 - DVU0896 0.62 +0.1 989,390 + DVU0896 0.69 -1.2 989,390 + DVU0896 0.69 +0.4
Or see this region's nucleotide sequence