Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0121 and yscR overlap by 4 nucleotides yscR and DVUA0123 are separated by 30 nucleotides DVUA0123 and DVUA0124 are separated by 15 nucleotides DVUA0124 and slt are separated by 10 nucleotides
DVUA0121: DVUA0121 - type III secretion system protein, YopQ family (TIGR), at 160,160 to 161,614
DVUA0121
DVUA0122: yscR - type III secretion system protein, YscR family (TIGR), at 161,611 to 162,261
yscR
DVUA0123: DVUA0123 - anti-anti-sigma factor (TIGR), at 162,292 to 162,627
DVUA0123
DVUA0124: DVUA0124 - sigma factor serine-protein kinase (TIGR), at 162,643 to 163,059
DVUA0124
DVUA0125: slt - transglycosylase, SLT family (TIGR), at 163,070 to 164,422
slt
Position (kb) 162
163 Strain fitness (log2 ratio) -2
-1
0
1
2 at 161.297 kb on + strand, within DVUA0121 at 161.454 kb on - strand, within DVUA0121 at 161.485 kb on + strand at 161.485 kb on + strand at 161.511 kb on + strand at 161.525 kb on + strand at 161.533 kb on - strand at 161.603 kb on - strand at 161.625 kb on - strand at 161.643 kb on + strand at 161.655 kb on + strand at 161.660 kb on + strand at 161.678 kb on - strand, within yscR at 161.697 kb on + strand, within yscR at 161.718 kb on - strand, within yscR at 161.783 kb on + strand, within yscR at 161.828 kb on + strand, within yscR at 161.858 kb on + strand, within yscR at 161.877 kb on - strand, within yscR at 161.898 kb on - strand, within yscR at 161.909 kb on - strand, within yscR at 161.959 kb on + strand, within yscR at 161.980 kb on - strand, within yscR at 162.011 kb on - strand, within yscR at 162.093 kb on + strand, within yscR at 162.094 kb on + strand, within yscR at 162.116 kb on + strand, within yscR at 162.174 kb on - strand, within yscR at 162.243 kb on + strand at 162.251 kb on - strand at 162.280 kb on + strand at 162.380 kb on + strand, within DVUA0123 at 162.402 kb on - strand, within DVUA0123 at 162.450 kb on - strand, within DVUA0123 at 162.491 kb on - strand, within DVUA0123 at 162.543 kb on + strand, within DVUA0123 at 163.210 kb on - strand, within slt at 163.216 kb on + strand, within slt at 163.235 kb on - strand, within slt at 163.281 kb on + strand, within slt at 163.384 kb on + strand, within slt at 163.417 kb on + strand, within slt at 163.423 kb on + strand, within slt at 163.436 kb on - strand, within slt at 163.441 kb on - strand, within slt at 163.451 kb on + strand, within slt at 163.536 kb on + strand, within slt at 163.613 kb on + strand, within slt
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 161,297 + DVUA0121 0.78 -0.3 161,454 - DVUA0121 0.89 +0.2 161,485 + +0.1 161,485 + +0.4 161,511 + -0.0 161,525 + -1.2 161,533 - -0.5 161,603 - -1.4 161,625 - -0.7 161,643 + +0.1 161,655 + -0.3 161,660 + -0.8 161,678 - yscR DVUA0122 0.10 +0.4 161,697 + yscR DVUA0122 0.13 -0.5 161,718 - yscR DVUA0122 0.16 +0.3 161,783 + yscR DVUA0122 0.26 +0.2 161,828 + yscR DVUA0122 0.33 -0.9 161,858 + yscR DVUA0122 0.38 +0.3 161,877 - yscR DVUA0122 0.41 -0.3 161,898 - yscR DVUA0122 0.44 -0.2 161,909 - yscR DVUA0122 0.46 +2.4 161,959 + yscR DVUA0122 0.53 +0.1 161,980 - yscR DVUA0122 0.57 -0.0 162,011 - yscR DVUA0122 0.61 -0.0 162,093 + yscR DVUA0122 0.74 -0.3 162,094 + yscR DVUA0122 0.74 +0.2 162,116 + yscR DVUA0122 0.78 -0.4 162,174 - yscR DVUA0122 0.86 +0.1 162,243 + -0.3 162,251 - -0.1 162,280 + -0.4 162,380 + DVUA0123 0.26 -2.0 162,402 - DVUA0123 0.33 +0.1 162,450 - DVUA0123 0.47 -1.1 162,491 - DVUA0123 0.59 -0.9 162,543 + DVUA0123 0.75 -0.1 163,210 - slt DVUA0125 0.10 +0.0 163,216 + slt DVUA0125 0.11 -0.2 163,235 - slt DVUA0125 0.12 -0.3 163,281 + slt DVUA0125 0.16 -0.1 163,384 + slt DVUA0125 0.23 -0.9 163,417 + slt DVUA0125 0.26 -2.0 163,423 + slt DVUA0125 0.26 -0.7 163,436 - slt DVUA0125 0.27 -1.2 163,441 - slt DVUA0125 0.27 -0.1 163,451 + slt DVUA0125 0.28 +0.6 163,536 + slt DVUA0125 0.34 -1.1 163,613 + slt DVUA0125 0.40 -0.2
Or see this region's nucleotide sequence