Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt b1396 and tRNA-Glu are separated by 143 nucleotides tRNA-Glu and DVU0602 are separated by 88 nucleotides DVU0602 and DVU0603 are separated by 228 nucleotides DVU0603 and DVU0604 overlap by 4 nucleotides DVU0604 and DVU0605 overlap by 18 nucleotides DVU0605 and DVU0606 are separated by 272 nucleotides
DVU0601: b1396 - phenylacetic acid degradation protein PaaI (TIGR), at 672,098 to 672,532
b1396
DVU_tRNA-Glu-1: tRNA-Glu - tRNA with anticodon TTC for Glu (VIMSS-AUTO), at 672,676 to 672,752
tRNA-Glu
DVU0602: DVU0602 - hypothetical protein (TIGR), at 672,841 to 673,203
DVU0602
DVU0603: DVU0603 - hypothetical protein (TIGR), at 673,432 to 673,698
DVU0603
DVU0604: DVU0604 - hypothetical protein (TIGR), at 673,695 to 673,826
DVU0604
DVU0605: DVU0605 - hypothetical protein (TIGR), at 673,809 to 673,937
DVU0605
DVU0606: DVU0606 - transcriptional regulator, ArsR family/methyltransferase, UbiE/COQ5 family (TIGR), at 674,210 to 675,133
DVU0606
Position (kb) 673
674 Strain fitness (log2 ratio) -2
-1
0
1 at 672.435 kb on + strand, within b1396 at 672.522 kb on - strand at 672.760 kb on - strand at 673.491 kb on + strand, within DVU0603 at 673.509 kb on - strand, within DVU0603 at 673.613 kb on - strand, within DVU0603 at 673.994 kb on - strand at 674.025 kb on - strand at 674.042 kb on - strand at 674.121 kb on - strand at 674.291 kb on - strand at 674.407 kb on + strand, within DVU0606 at 674.437 kb on - strand, within DVU0606 at 674.454 kb on + strand, within DVU0606 at 674.497 kb on + strand, within DVU0606 at 674.532 kb on + strand, within DVU0606 at 674.579 kb on + strand, within DVU0606 at 674.602 kb on - strand, within DVU0606 at 674.622 kb on - strand, within DVU0606 at 674.644 kb on + strand, within DVU0606
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 672,435 + b1396 DVU0601 0.77 -0.1 672,522 - +0.0 672,760 - +0.4 673,491 + DVU0603 0.22 -0.4 673,509 - DVU0603 0.29 +0.4 673,613 - DVU0603 0.68 -0.7 673,994 - -0.9 674,025 - -0.1 674,042 - -0.1 674,121 - +0.1 674,291 - -0.2 674,407 + DVU0606 0.21 -0.9 674,437 - DVU0606 0.25 -0.8 674,454 + DVU0606 0.26 +0.2 674,497 + DVU0606 0.31 -0.1 674,532 + DVU0606 0.35 +0.0 674,579 + DVU0606 0.40 +1.2 674,602 - DVU0606 0.42 +0.7 674,622 - DVU0606 0.45 -2.0 674,644 + DVU0606 0.47 -0.2
Or see this region's nucleotide sequence