Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpsO and truB are separated by 24 nucleotides truB and DVU0506 are separated by 4 nucleotides DVU0506 and DVU0507 are separated by 17 nucleotides DVU0507 and infB are separated by 103 nucleotides
DVU0504: rpsO - ribosomal protein S15 (TIGR), at 577,418 to 577,687
rpsO
DVU0505: truB - tRNA pseudouridine synthase B (TIGR), at 577,712 to 578,626
truB
DVU0506: DVU0506 - DHH family protein (TIGR), at 578,631 to 579,632
DVU0506
DVU0507: DVU0507 - conserved hypothetical protein (TIGR), at 579,650 to 579,940
DVU0507
DVU0508: infB - translation initiation factor IF-2 (TIGR), at 580,044 to 583,283
infB
Position (kb) 578
579
580 Strain fitness (log2 ratio) -2
-1
0
1 at 578.039 kb on + strand, within truB at 578.061 kb on - strand, within truB at 578.252 kb on - strand, within truB at 578.270 kb on - strand, within truB at 578.339 kb on + strand, within truB at 578.389 kb on - strand, within truB at 578.401 kb on + strand, within truB at 578.423 kb on - strand, within truB at 578.436 kb on + strand, within truB at 578.534 kb on - strand, within truB at 578.580 kb on + strand at 578.593 kb on - strand at 578.642 kb on - strand at 578.654 kb on - strand at 578.729 kb on - strand at 578.781 kb on - strand, within DVU0506 at 578.846 kb on + strand, within DVU0506 at 578.936 kb on - strand, within DVU0506 at 579.160 kb on + strand, within DVU0506 at 579.224 kb on + strand, within DVU0506 at 579.372 kb on + strand, within DVU0506 at 579.372 kb on + strand, within DVU0506 at 579.388 kb on + strand, within DVU0506 at 579.429 kb on - strand, within DVU0506 at 579.429 kb on - strand, within DVU0506 at 579.429 kb on - strand, within DVU0506 at 579.677 kb on + strand at 579.717 kb on - strand, within DVU0507 at 579.737 kb on + strand, within DVU0507 at 579.853 kb on + strand, within DVU0507 at 579.921 kb on - strand at 579.966 kb on - strand at 580.013 kb on - strand at 580.044 kb on + strand at 580.044 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 578,039 + truB DVU0505 0.36 +0.4 578,061 - truB DVU0505 0.38 -0.9 578,252 - truB DVU0505 0.59 -0.6 578,270 - truB DVU0505 0.61 -0.8 578,339 + truB DVU0505 0.69 -2.1 578,389 - truB DVU0505 0.74 -0.2 578,401 + truB DVU0505 0.75 -0.5 578,423 - truB DVU0505 0.78 +0.2 578,436 + truB DVU0505 0.79 -0.0 578,534 - truB DVU0505 0.90 -0.3 578,580 + -0.1 578,593 - +1.3 578,642 - -0.7 578,654 - +0.4 578,729 - -0.6 578,781 - DVU0506 0.15 -0.5 578,846 + DVU0506 0.21 +1.0 578,936 - DVU0506 0.30 -0.6 579,160 + DVU0506 0.53 -0.1 579,224 + DVU0506 0.59 -1.5 579,372 + DVU0506 0.74 -0.5 579,372 + DVU0506 0.74 -0.0 579,388 + DVU0506 0.76 -1.1 579,429 - DVU0506 0.80 -1.1 579,429 - DVU0506 0.80 +0.0 579,429 - DVU0506 0.80 -0.3 579,677 + -0.2 579,717 - DVU0507 0.23 +0.5 579,737 + DVU0507 0.30 -1.4 579,853 + DVU0507 0.70 +1.3 579,921 - -0.5 579,966 - +0.2 580,013 - -0.5 580,044 + -0.4 580,044 + -0.4
Or see this region's nucleotide sequence