Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0486 and purE are separated by 299 nucleotides purE and purD are separated by 13 nucleotides purD and paaK-1 are separated by 167 nucleotides
DVU0486: DVU0486 - hypothetical protein (TIGR), at 554,000 to 554,329
DVU0486
DVU0487: purE - phosphoribosylaminoimidazole carboxylase, catalytic subunit (TIGR), at 554,629 to 555,129
purE
DVU0488: purD - phosphoribosylamine--glycine ligase (TIGR), at 555,143 to 556,417
purD
DVU0489: paaK-1 - phenylacetate-coenzyme A ligase (TIGR), at 556,585 to 557,883
paaK-1
Position (kb) 555
556
557 Strain fitness (log2 ratio) -2
-1
0
1
2 at 554.149 kb on - strand, within DVU0486 at 554.162 kb on + strand, within DVU0486 at 554.250 kb on + strand, within DVU0486 at 554.268 kb on - strand, within DVU0486 at 554.331 kb on - strand at 554.408 kb on + strand at 554.423 kb on + strand at 554.457 kb on - strand at 554.514 kb on + strand at 554.631 kb on + strand at 555.142 kb on + strand at 555.160 kb on - strand at 555.327 kb on + strand, within purD at 555.384 kb on + strand, within purD at 555.384 kb on + strand, within purD at 555.392 kb on - strand, within purD at 555.407 kb on + strand, within purD at 555.808 kb on - strand, within purD at 555.860 kb on - strand, within purD at 555.890 kb on + strand, within purD at 555.958 kb on - strand, within purD at 556.180 kb on + strand, within purD at 556.204 kb on + strand, within purD at 556.336 kb on + strand at 556.431 kb on - strand at 556.498 kb on + strand at 556.575 kb on + strand at 556.629 kb on + strand at 556.700 kb on + strand at 556.776 kb on - strand, within paaK-1 at 556.823 kb on - strand, within paaK-1 at 556.845 kb on + strand, within paaK-1 at 556.875 kb on + strand, within paaK-1 at 556.887 kb on + strand, within paaK-1 at 556.887 kb on + strand, within paaK-1 at 556.887 kb on + strand, within paaK-1 at 556.889 kb on + strand, within paaK-1 at 556.890 kb on - strand, within paaK-1 at 556.895 kb on - strand, within paaK-1 at 556.895 kb on - strand, within paaK-1 at 556.897 kb on - strand, within paaK-1 at 556.921 kb on - strand, within paaK-1 at 556.968 kb on - strand, within paaK-1 at 556.972 kb on + strand, within paaK-1 at 556.988 kb on + strand, within paaK-1 at 557.005 kb on + strand, within paaK-1 at 557.015 kb on + strand, within paaK-1 at 557.145 kb on + strand, within paaK-1 at 557.145 kb on + strand, within paaK-1 at 557.194 kb on - strand, within paaK-1 at 557.194 kb on - strand, within paaK-1 at 557.196 kb on + strand, within paaK-1 at 557.254 kb on - strand, within paaK-1 at 557.266 kb on + strand, within paaK-1 at 557.321 kb on + strand, within paaK-1 at 557.400 kb on + strand, within paaK-1
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 554,149 - DVU0486 0.45 -0.5 554,162 + DVU0486 0.49 -0.1 554,250 + DVU0486 0.76 -0.2 554,268 - DVU0486 0.81 -0.3 554,331 - +0.1 554,408 + -0.4 554,423 + -0.3 554,457 - +0.5 554,514 + -0.3 554,631 + -1.1 555,142 + -0.3 555,160 - -0.1 555,327 + purD DVU0488 0.14 -0.3 555,384 + purD DVU0488 0.19 -2.3 555,384 + purD DVU0488 0.19 -1.4 555,392 - purD DVU0488 0.20 -2.4 555,407 + purD DVU0488 0.21 -1.3 555,808 - purD DVU0488 0.52 -0.0 555,860 - purD DVU0488 0.56 -0.2 555,890 + purD DVU0488 0.59 -2.7 555,958 - purD DVU0488 0.64 -1.5 556,180 + purD DVU0488 0.81 -0.5 556,204 + purD DVU0488 0.83 -2.2 556,336 + -1.0 556,431 - -1.9 556,498 + +0.4 556,575 + -0.9 556,629 + -1.2 556,700 + -0.3 556,776 - paaK-1 DVU0489 0.15 +0.0 556,823 - paaK-1 DVU0489 0.18 +0.0 556,845 + paaK-1 DVU0489 0.20 -0.4 556,875 + paaK-1 DVU0489 0.22 +0.1 556,887 + paaK-1 DVU0489 0.23 -0.0 556,887 + paaK-1 DVU0489 0.23 +0.3 556,887 + paaK-1 DVU0489 0.23 -0.0 556,889 + paaK-1 DVU0489 0.23 -0.1 556,890 - paaK-1 DVU0489 0.23 +0.3 556,895 - paaK-1 DVU0489 0.24 -0.5 556,895 - paaK-1 DVU0489 0.24 +0.2 556,897 - paaK-1 DVU0489 0.24 -1.5 556,921 - paaK-1 DVU0489 0.26 -0.9 556,968 - paaK-1 DVU0489 0.29 -0.8 556,972 + paaK-1 DVU0489 0.30 +2.3 556,988 + paaK-1 DVU0489 0.31 +1.5 557,005 + paaK-1 DVU0489 0.32 -1.8 557,015 + paaK-1 DVU0489 0.33 -0.3 557,145 + paaK-1 DVU0489 0.43 +0.4 557,145 + paaK-1 DVU0489 0.43 +0.1 557,194 - paaK-1 DVU0489 0.47 +0.3 557,194 - paaK-1 DVU0489 0.47 +0.6 557,196 + paaK-1 DVU0489 0.47 +0.2 557,254 - paaK-1 DVU0489 0.52 -0.5 557,266 + paaK-1 DVU0489 0.52 -0.1 557,321 + paaK-1 DVU0489 0.57 -2.3 557,400 + paaK-1 DVU0489 0.63 -0.9
Or see this region's nucleotide sequence