Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt carB and DVU0163 are separated by 438 nucleotides DVU0163 and DVU0164 are separated by 63 nucleotides
DVU0162: carB - carbamoyl-phosphate synthase, large subunit (TIGR), at 199,861 to 203,094
carB
DVU0163: DVU0163 - lipoprotein, putative (TIGR), at 203,533 to 204,093
DVU0163
DVU0164: DVU0164 - cation efflux family protein (TIGR), at 204,157 to 205,065
DVU0164
Position (kb) 203
204
205 Strain fitness (log2 ratio) -2
-1
0
1 at 203.740 kb on + strand, within DVU0163 at 203.887 kb on - strand, within DVU0163 at 203.955 kb on - strand, within DVU0163 at 204.002 kb on + strand, within DVU0163 at 204.004 kb on - strand, within DVU0163 at 204.057 kb on + strand at 204.143 kb on - strand at 204.158 kb on + strand at 204.158 kb on + strand at 204.158 kb on + strand at 204.158 kb on + strand at 204.166 kb on - strand at 204.168 kb on + strand at 204.168 kb on + strand at 204.168 kb on + strand at 204.168 kb on + strand at 204.176 kb on - strand at 204.181 kb on - strand at 204.192 kb on + strand at 204.208 kb on - strand at 204.238 kb on - strand at 204.242 kb on - strand at 204.336 kb on - strand, within DVU0164 at 204.355 kb on - strand, within DVU0164 at 204.491 kb on + strand, within DVU0164 at 204.499 kb on - strand, within DVU0164 at 204.504 kb on - strand, within DVU0164 at 204.505 kb on + strand, within DVU0164 at 204.513 kb on - strand, within DVU0164 at 204.551 kb on + strand, within DVU0164 at 204.580 kb on - strand, within DVU0164 at 204.696 kb on + strand, within DVU0164 at 204.706 kb on - strand, within DVU0164 at 204.722 kb on - strand, within DVU0164 at 204.732 kb on - strand, within DVU0164 at 204.732 kb on - strand, within DVU0164 at 204.788 kb on - strand, within DVU0164 at 204.922 kb on - strand, within DVU0164 at 204.931 kb on - strand, within DVU0164 at 204.958 kb on - strand, within DVU0164 at 204.979 kb on - strand at 205.008 kb on - strand at 205.018 kb on + strand at 205.031 kb on - strand at 205.061 kb on + strand at 205.061 kb on + strand at 205.088 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 203,740 + DVU0163 0.37 +0.1 203,887 - DVU0163 0.63 -0.5 203,955 - DVU0163 0.75 +0.9 204,002 + DVU0163 0.84 +0.2 204,004 - DVU0163 0.84 +1.2 204,057 + +0.2 204,143 - +0.0 204,158 + -0.2 204,158 + -0.1 204,158 + -0.1 204,158 + -0.3 204,166 - -0.5 204,168 + -0.6 204,168 + +1.3 204,168 + +0.3 204,168 + +0.2 204,176 - -0.0 204,181 - -1.0 204,192 + +0.2 204,208 - -0.2 204,238 - +0.1 204,242 - +0.0 204,336 - DVU0164 0.20 +0.4 204,355 - DVU0164 0.22 -0.2 204,491 + DVU0164 0.37 -0.1 204,499 - DVU0164 0.38 -0.8 204,504 - DVU0164 0.38 -0.5 204,505 + DVU0164 0.38 -2.3 204,513 - DVU0164 0.39 +0.4 204,551 + DVU0164 0.43 +0.1 204,580 - DVU0164 0.47 -0.4 204,696 + DVU0164 0.59 -0.5 204,706 - DVU0164 0.60 -0.1 204,722 - DVU0164 0.62 +0.2 204,732 - DVU0164 0.63 +0.1 204,732 - DVU0164 0.63 -0.0 204,788 - DVU0164 0.69 +0.7 204,922 - DVU0164 0.84 +0.4 204,931 - DVU0164 0.85 -0.5 204,958 - DVU0164 0.88 +1.0 204,979 - +0.1 205,008 - -0.0 205,018 + -0.0 205,031 - -0.2 205,061 + +1.3 205,061 + -0.2 205,088 - +0.3
Or see this region's nucleotide sequence