Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt acrA and DVU0061 are separated by 15 nucleotides DVU0061 and DVU0062 overlap by 4 nucleotides
DVU0060: acrA - efflux transporter, RND family, MFP subunit (TIGR), at 69,540 to 70,724
acrA
DVU0061: DVU0061 - multidrug resistance protein, putative (TIGR), at 70,740 to 73,880
DVU0061
DVU0062: DVU0062 - RND efflux system, outer membrane protein, NodT family (TIGR), at 73,877 to 75,391
DVU0062
Position (kb)
70
71
72
73
74 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 69.787 kb on - strand, within acrA at 69.806 kb on - strand, within acrA at 69.850 kb on - strand, within acrA at 69.850 kb on - strand, within acrA at 69.863 kb on + strand, within acrA at 69.885 kb on + strand, within acrA at 69.958 kb on + strand, within acrA at 70.018 kb on - strand, within acrA at 70.020 kb on + strand, within acrA at 70.020 kb on + strand, within acrA at 70.028 kb on - strand, within acrA at 70.030 kb on + strand, within acrA at 70.204 kb on + strand, within acrA at 70.211 kb on + strand, within acrA at 70.214 kb on - strand, within acrA at 70.256 kb on - strand, within acrA at 70.302 kb on + strand, within acrA at 70.302 kb on + strand, within acrA at 70.310 kb on - strand, within acrA at 70.310 kb on - strand, within acrA at 70.310 kb on - strand, within acrA at 70.357 kb on + strand, within acrA at 70.436 kb on + strand, within acrA at 70.641 kb on - strand at 70.676 kb on - strand at 70.732 kb on - strand at 70.876 kb on - strand at 70.946 kb on + strand at 70.954 kb on - strand at 70.954 kb on - strand at 70.985 kb on - strand at 71.046 kb on - strand at 71.046 kb on - strand at 71.096 kb on + strand, within DVU0061 at 71.127 kb on - strand, within DVU0061 at 71.186 kb on + strand, within DVU0061 at 71.219 kb on + strand, within DVU0061 at 71.238 kb on - strand, within DVU0061 at 71.253 kb on + strand, within DVU0061 at 71.273 kb on + strand, within DVU0061 at 71.280 kb on + strand, within DVU0061 at 71.285 kb on + strand, within DVU0061 at 71.285 kb on + strand, within DVU0061 at 71.286 kb on + strand, within DVU0061 at 71.349 kb on + strand, within DVU0061 at 71.445 kb on + strand, within DVU0061 at 71.449 kb on + strand, within DVU0061 at 71.457 kb on - strand, within DVU0061 at 71.470 kb on + strand, within DVU0061 at 71.472 kb on + strand, within DVU0061 at 71.472 kb on - strand, within DVU0061 at 71.480 kb on - strand, within DVU0061 at 71.496 kb on - strand, within DVU0061 at 71.496 kb on - strand, within DVU0061 at 71.515 kb on + strand, within DVU0061 at 71.536 kb on - strand, within DVU0061 at 71.543 kb on - strand, within DVU0061 at 71.565 kb on + strand, within DVU0061 at 71.684 kb on - strand, within DVU0061 at 71.711 kb on - strand, within DVU0061 at 71.712 kb on - strand, within DVU0061 at 71.713 kb on + strand, within DVU0061 at 71.721 kb on - strand, within DVU0061 at 71.721 kb on - strand, within DVU0061 at 71.746 kb on - strand, within DVU0061 at 71.897 kb on - strand, within DVU0061 at 71.897 kb on - strand, within DVU0061 at 72.045 kb on + strand, within DVU0061 at 72.046 kb on - strand, within DVU0061 at 72.101 kb on + strand, within DVU0061 at 72.135 kb on + strand, within DVU0061 at 72.173 kb on + strand, within DVU0061 at 72.173 kb on + strand, within DVU0061 at 72.173 kb on + strand, within DVU0061 at 72.181 kb on - strand, within DVU0061 at 72.214 kb on - strand, within DVU0061 at 72.219 kb on - strand, within DVU0061 at 72.305 kb on + strand, within DVU0061 at 72.432 kb on + strand, within DVU0061 at 72.432 kb on + strand, within DVU0061 at 72.440 kb on - strand, within DVU0061 at 72.454 kb on + strand, within DVU0061 at 72.477 kb on + strand, within DVU0061 at 72.477 kb on + strand, within DVU0061 at 72.485 kb on - strand, within DVU0061 at 72.515 kb on - strand, within DVU0061 at 72.547 kb on + strand, within DVU0061 at 72.565 kb on + strand, within DVU0061 at 72.601 kb on - strand, within DVU0061 at 72.611 kb on - strand, within DVU0061 at 72.627 kb on - strand, within DVU0061 at 72.628 kb on + strand, within DVU0061 at 72.638 kb on + strand, within DVU0061 at 72.702 kb on - strand, within DVU0061 at 72.757 kb on - strand, within DVU0061 at 72.766 kb on + strand, within DVU0061 at 72.766 kb on + strand, within DVU0061 at 72.780 kb on + strand, within DVU0061 at 72.788 kb on - strand, within DVU0061 at 72.846 kb on + strand, within DVU0061 at 72.846 kb on + strand, within DVU0061 at 72.846 kb on + strand, within DVU0061 at 72.846 kb on + strand, within DVU0061 at 72.846 kb on + strand, within DVU0061 at 72.846 kb on + strand, within DVU0061 at 72.854 kb on - strand, within DVU0061 at 72.854 kb on - strand, within DVU0061 at 72.854 kb on - strand, within DVU0061 at 72.854 kb on - strand, within DVU0061 at 72.913 kb on - strand, within DVU0061 at 72.935 kb on - strand, within DVU0061 at 72.978 kb on - strand, within DVU0061 at 73.000 kb on + strand, within DVU0061 at 73.100 kb on - strand, within DVU0061 at 73.138 kb on - strand, within DVU0061 at 73.144 kb on + strand, within DVU0061 at 73.154 kb on + strand, within DVU0061 at 73.205 kb on + strand, within DVU0061 at 73.234 kb on + strand, within DVU0061 at 73.242 kb on - strand, within DVU0061 at 73.242 kb on - strand, within DVU0061 at 73.321 kb on + strand, within DVU0061 at 73.356 kb on + strand, within DVU0061 at 73.364 kb on - strand, within DVU0061 at 73.439 kb on - strand, within DVU0061 at 73.457 kb on + strand, within DVU0061 at 73.465 kb on - strand, within DVU0061 at 73.482 kb on - strand, within DVU0061 at 73.522 kb on - strand, within DVU0061 at 73.595 kb on + strand at 73.682 kb on - strand at 73.710 kb on - strand at 73.821 kb on - strand at 73.843 kb on + strand at 73.898 kb on + strand at 73.967 kb on - strand at 73.967 kb on - strand at 74.035 kb on + strand, within DVU0062 at 74.043 kb on - strand, within DVU0062 at 74.051 kb on + strand, within DVU0062 at 74.066 kb on - strand, within DVU0062 at 74.110 kb on - strand, within DVU0062 at 74.179 kb on + strand, within DVU0062 at 74.187 kb on - strand, within DVU0062 at 74.254 kb on + strand, within DVU0062 at 74.267 kb on + strand, within DVU0062 at 74.354 kb on + strand, within DVU0062 at 74.452 kb on - strand, within DVU0062 at 74.486 kb on - strand, within DVU0062 at 74.519 kb on - strand, within DVU0062 at 74.540 kb on + strand, within DVU0062 at 74.561 kb on - strand, within DVU0062 at 74.561 kb on - strand, within DVU0062 at 74.747 kb on - strand, within DVU0062 at 74.861 kb on - strand, within DVU0062 at 74.861 kb on - strand, within DVU0062 at 74.866 kb on - strand, within DVU0062
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 69,787 - acrA DVU0060 0.21 -0.5 69,806 - acrA DVU0060 0.22 -0.6 69,850 - acrA DVU0060 0.26 -2.0 69,850 - acrA DVU0060 0.26 -0.8 69,863 + acrA DVU0060 0.27 -1.9 69,885 + acrA DVU0060 0.29 -0.0 69,958 + acrA DVU0060 0.35 +0.5 70,018 - acrA DVU0060 0.40 +1.0 70,020 + acrA DVU0060 0.41 -0.5 70,020 + acrA DVU0060 0.41 -0.3 70,028 - acrA DVU0060 0.41 -1.4 70,030 + acrA DVU0060 0.41 -0.7 70,204 + acrA DVU0060 0.56 +0.2 70,211 + acrA DVU0060 0.57 -0.9 70,214 - acrA DVU0060 0.57 +0.0 70,256 - acrA DVU0060 0.60 -0.3 70,302 + acrA DVU0060 0.64 +0.1 70,302 + acrA DVU0060 0.64 +0.1 70,310 - acrA DVU0060 0.65 -0.2 70,310 - acrA DVU0060 0.65 -0.3 70,310 - acrA DVU0060 0.65 -0.2 70,357 + acrA DVU0060 0.69 +0.2 70,436 + acrA DVU0060 0.76 -0.1 70,641 - -0.6 70,676 - -0.3 70,732 - -0.3 70,876 - +0.3 70,946 + +0.1 70,954 - +0.2 70,954 - -0.2 70,985 - -0.4 71,046 - -0.4 71,046 - -1.2 71,096 + DVU0061 0.11 -0.1 71,127 - DVU0061 0.12 -0.5 71,186 + DVU0061 0.14 +0.3 71,219 + DVU0061 0.15 -0.2 71,238 - DVU0061 0.16 +2.2 71,253 + DVU0061 0.16 -0.0 71,273 + DVU0061 0.17 +0.4 71,280 + DVU0061 0.17 -1.0 71,285 + DVU0061 0.17 -1.4 71,285 + DVU0061 0.17 +0.2 71,286 + DVU0061 0.17 +0.1 71,349 + DVU0061 0.19 -0.2 71,445 + DVU0061 0.22 +0.0 71,449 + DVU0061 0.23 -0.7 71,457 - DVU0061 0.23 +0.4 71,470 + DVU0061 0.23 +0.1 71,472 + DVU0061 0.23 -0.2 71,472 - DVU0061 0.23 -0.5 71,480 - DVU0061 0.24 -0.1 71,496 - DVU0061 0.24 -0.5 71,496 - DVU0061 0.24 -2.2 71,515 + DVU0061 0.25 +0.7 71,536 - DVU0061 0.25 -1.6 71,543 - DVU0061 0.26 -0.7 71,565 + DVU0061 0.26 +0.1 71,684 - DVU0061 0.30 -0.6 71,711 - DVU0061 0.31 -0.7 71,712 - DVU0061 0.31 -0.8 71,713 + DVU0061 0.31 -0.5 71,721 - DVU0061 0.31 -1.0 71,721 - DVU0061 0.31 +0.4 71,746 - DVU0061 0.32 -0.0 71,897 - DVU0061 0.37 -0.4 71,897 - DVU0061 0.37 +0.4 72,045 + DVU0061 0.42 +0.7 72,046 - DVU0061 0.42 -0.9 72,101 + DVU0061 0.43 +0.1 72,135 + DVU0061 0.44 +0.4 72,173 + DVU0061 0.46 +0.2 72,173 + DVU0061 0.46 -1.7 72,173 + DVU0061 0.46 +0.2 72,181 - DVU0061 0.46 +0.0 72,214 - DVU0061 0.47 -0.2 72,219 - DVU0061 0.47 -1.2 72,305 + DVU0061 0.50 -0.2 72,432 + DVU0061 0.54 -1.0 72,432 + DVU0061 0.54 +0.2 72,440 - DVU0061 0.54 -1.3 72,454 + DVU0061 0.55 -0.3 72,477 + DVU0061 0.55 -0.9 72,477 + DVU0061 0.55 -0.9 72,485 - DVU0061 0.56 -0.3 72,515 - DVU0061 0.57 +0.2 72,547 + DVU0061 0.58 -0.2 72,565 + DVU0061 0.58 -0.1 72,601 - DVU0061 0.59 -0.4 72,611 - DVU0061 0.60 -0.3 72,627 - DVU0061 0.60 -1.3 72,628 + DVU0061 0.60 +0.4 72,638 + DVU0061 0.60 -0.0 72,702 - DVU0061 0.62 -0.6 72,757 - DVU0061 0.64 +0.4 72,766 + DVU0061 0.65 -0.5 72,766 + DVU0061 0.65 -0.5 72,780 + DVU0061 0.65 -0.7 72,788 - DVU0061 0.65 -1.6 72,846 + DVU0061 0.67 -0.5 72,846 + DVU0061 0.67 -0.7 72,846 + DVU0061 0.67 -0.6 72,846 + DVU0061 0.67 -1.5 72,846 + DVU0061 0.67 -0.5 72,846 + DVU0061 0.67 -0.8 72,854 - DVU0061 0.67 -1.4 72,854 - DVU0061 0.67 +0.1 72,854 - DVU0061 0.67 +1.1 72,854 - DVU0061 0.67 -0.3 72,913 - DVU0061 0.69 -0.5 72,935 - DVU0061 0.70 -0.8 72,978 - DVU0061 0.71 -1.2 73,000 + DVU0061 0.72 -1.6 73,100 - DVU0061 0.75 -0.9 73,138 - DVU0061 0.76 -0.3 73,144 + DVU0061 0.77 -0.1 73,154 + DVU0061 0.77 -1.3 73,205 + DVU0061 0.78 -1.5 73,234 + DVU0061 0.79 -1.0 73,242 - DVU0061 0.80 -3.8 73,242 - DVU0061 0.80 -0.2 73,321 + DVU0061 0.82 -1.6 73,356 + DVU0061 0.83 -0.5 73,364 - DVU0061 0.84 -1.2 73,439 - DVU0061 0.86 -1.2 73,457 + DVU0061 0.87 -0.7 73,465 - DVU0061 0.87 +0.0 73,482 - DVU0061 0.87 -1.7 73,522 - DVU0061 0.89 +0.1 73,595 + -0.2 73,682 - -0.9 73,710 - -0.6 73,821 - +1.2 73,843 + +0.2 73,898 + -1.2 73,967 - -0.8 73,967 - -0.5 74,035 + DVU0062 0.10 -1.4 74,043 - DVU0062 0.11 -2.3 74,051 + DVU0062 0.11 -0.6 74,066 - DVU0062 0.12 -1.2 74,110 - DVU0062 0.15 -1.2 74,179 + DVU0062 0.20 +1.8 74,187 - DVU0062 0.20 -0.5 74,254 + DVU0062 0.25 -0.4 74,267 + DVU0062 0.26 +2.2 74,354 + DVU0062 0.31 -1.8 74,452 - DVU0062 0.38 +0.4 74,486 - DVU0062 0.40 -2.6 74,519 - DVU0062 0.42 -1.6 74,540 + DVU0062 0.44 -0.7 74,561 - DVU0062 0.45 -0.0 74,561 - DVU0062 0.45 -0.5 74,747 - DVU0062 0.57 -0.6 74,861 - DVU0062 0.65 +0.7 74,861 - DVU0062 0.65 -1.3 74,866 - DVU0062 0.65 +0.2
Or see this region's nucleotide sequence