Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0012 and DVU0013 are separated by 160 nucleotides DVU0013 and infA-1 are separated by 199 nucleotides infA-1 and lgt are separated by 115 nucleotides
DVU0012: DVU0012 - hypothetical protein (TIGR), at 15,099 to 16,301
DVU0012
DVU0013: DVU0013 - sensory box histidine kinase (TIGR), at 16,462 to 18,270
DVU0013
DVU0014: infA-1 - translation initiation factor IF-1 (TIGR), at 18,470 to 18,688
infA-1
DVU0015: lgt - prolipoprotein diacylglyceryl transferase (TIGR), at 18,804 to 19,595
lgt
Position (kb)
16
17
18
19 Strain fitness (log2 ratio)
-2
-1
0
1 at 15.502 kb on - strand, within DVU0012 at 15.522 kb on - strand, within DVU0012 at 15.557 kb on - strand, within DVU0012 at 15.733 kb on - strand, within DVU0012 at 15.733 kb on - strand, within DVU0012 at 15.838 kb on - strand, within DVU0012 at 15.878 kb on + strand, within DVU0012 at 15.986 kb on - strand, within DVU0012 at 16.095 kb on + strand, within DVU0012 at 16.150 kb on + strand, within DVU0012 at 16.152 kb on + strand, within DVU0012 at 16.153 kb on - strand, within DVU0012 at 16.173 kb on + strand, within DVU0012 at 16.188 kb on - strand at 16.288 kb on - strand at 16.288 kb on - strand at 16.314 kb on + strand at 16.367 kb on - strand at 16.425 kb on - strand at 16.444 kb on + strand at 16.461 kb on + strand at 16.467 kb on - strand at 16.699 kb on - strand, within DVU0013 at 16.776 kb on + strand, within DVU0013 at 16.835 kb on + strand, within DVU0013 at 16.843 kb on - strand, within DVU0013 at 16.843 kb on - strand, within DVU0013 at 16.848 kb on + strand, within DVU0013 at 16.853 kb on + strand, within DVU0013 at 16.861 kb on - strand, within DVU0013 at 16.893 kb on + strand, within DVU0013 at 16.992 kb on - strand, within DVU0013 at 17.060 kb on + strand, within DVU0013 at 17.115 kb on - strand, within DVU0013 at 17.237 kb on - strand, within DVU0013 at 17.772 kb on - strand, within DVU0013 at 17.781 kb on + strand, within DVU0013 at 17.803 kb on + strand, within DVU0013 at 17.810 kb on + strand, within DVU0013 at 17.932 kb on + strand, within DVU0013 at 18.039 kb on - strand, within DVU0013 at 18.124 kb on + strand at 18.124 kb on + strand at 18.154 kb on + strand at 18.206 kb on - strand at 18.290 kb on + strand at 18.395 kb on + strand at 18.457 kb on - strand at 18.516 kb on - strand, within infA-1 at 18.635 kb on + strand, within infA-1 at 18.635 kb on + strand, within infA-1 at 18.656 kb on + strand, within infA-1 at 18.747 kb on - strand at 18.811 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 15,502 - DVU0012 0.33 +0.2 15,522 - DVU0012 0.35 +0.2 15,557 - DVU0012 0.38 +0.6 15,733 - DVU0012 0.53 +0.2 15,733 - DVU0012 0.53 +0.1 15,838 - DVU0012 0.61 +0.2 15,878 + DVU0012 0.65 -0.2 15,986 - DVU0012 0.74 +0.2 16,095 + DVU0012 0.83 +0.2 16,150 + DVU0012 0.87 -2.6 16,152 + DVU0012 0.88 +0.0 16,153 - DVU0012 0.88 +0.6 16,173 + DVU0012 0.89 -0.8 16,188 - -0.0 16,288 - +0.1 16,288 - +0.0 16,314 + -2.4 16,367 - -0.6 16,425 - +1.3 16,444 + -0.8 16,461 + -0.0 16,467 - -0.6 16,699 - DVU0013 0.13 -0.7 16,776 + DVU0013 0.17 -0.1 16,835 + DVU0013 0.21 -0.1 16,843 - DVU0013 0.21 -1.2 16,843 - DVU0013 0.21 -2.2 16,848 + DVU0013 0.21 -1.1 16,853 + DVU0013 0.22 -0.9 16,861 - DVU0013 0.22 -0.4 16,893 + DVU0013 0.24 +0.4 16,992 - DVU0013 0.29 +0.4 17,060 + DVU0013 0.33 -0.6 17,115 - DVU0013 0.36 -2.3 17,237 - DVU0013 0.43 -0.1 17,772 - DVU0013 0.72 -1.0 17,781 + DVU0013 0.73 -0.6 17,803 + DVU0013 0.74 +0.0 17,810 + DVU0013 0.75 -0.6 17,932 + DVU0013 0.81 -1.1 18,039 - DVU0013 0.87 -2.2 18,124 + +0.4 18,124 + +0.0 18,154 + -2.7 18,206 - -0.3 18,290 + +0.3 18,395 + -0.7 18,457 - -0.8 18,516 - infA-1 DVU0014 0.21 -0.3 18,635 + infA-1 DVU0014 0.75 -0.1 18,635 + infA-1 DVU0014 0.75 -1.0 18,656 + infA-1 DVU0014 0.85 -0.3 18,747 - -0.2 18,811 + +1.7
Or see this region's nucleotide sequence