Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ackA and DVU3031 are separated by 99 nucleotides DVU3031 and DVU3032 overlap by 4 nucleotides DVU3032 and DVU3033 are separated by 3 nucleotides
DVU3030: ackA - acetate kinase (TIGR), at 3,154,356 to 3,155,564
ackA
DVU3031: DVU3031 - conserved hypothetical protein (TIGR), at 3,155,664 to 3,156,755
DVU3031
DVU3032: DVU3032 - conserved hypothetical protein (TIGR), at 3,156,752 to 3,157,381
DVU3032
DVU3033: DVU3033 - iron-sulfur cluster-binding protein (TIGR), at 3,157,385 to 3,159,538
DVU3033
Position (kb)
3155
3156
3157 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3155.582 kb on - strand at 3155.614 kb on + strand at 3155.684 kb on - strand at 3155.709 kb on + strand at 3155.834 kb on + strand, within DVU3031 at 3155.952 kb on + strand, within DVU3031 at 3155.956 kb on + strand, within DVU3031 at 3155.960 kb on - strand, within DVU3031 at 3156.019 kb on - strand, within DVU3031 at 3156.019 kb on - strand, within DVU3031 at 3156.034 kb on + strand, within DVU3031 at 3156.061 kb on + strand, within DVU3031 at 3156.061 kb on + strand, within DVU3031 at 3156.061 kb on + strand, within DVU3031 at 3156.145 kb on - strand, within DVU3031 at 3156.145 kb on - strand, within DVU3031 at 3156.276 kb on - strand, within DVU3031 at 3156.528 kb on - strand, within DVU3031 at 3156.542 kb on + strand, within DVU3031 at 3156.607 kb on - strand, within DVU3031 at 3156.648 kb on - strand at 3156.656 kb on + strand at 3156.761 kb on + strand at 3156.792 kb on + strand at 3156.832 kb on - strand, within DVU3032 at 3156.863 kb on - strand, within DVU3032 at 3157.002 kb on + strand, within DVU3032 at 3157.049 kb on + strand, within DVU3032 at 3157.143 kb on + strand, within DVU3032 at 3157.235 kb on - strand, within DVU3032 at 3157.288 kb on - strand, within DVU3032 at 3157.342 kb on - strand at 3157.383 kb on - strand at 3157.383 kb on - strand at 3157.383 kb on - strand at 3157.401 kb on - strand at 3157.597 kb on + strand at 3157.597 kb on + strand at 3157.597 kb on + strand at 3157.597 kb on + strand at 3157.597 kb on + strand at 3157.605 kb on - strand, within DVU3033 at 3157.605 kb on - strand, within DVU3033 at 3157.623 kb on + strand, within DVU3033 at 3157.631 kb on - strand, within DVU3033
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 3,155,582 - +0.1 3,155,614 + -0.4 3,155,684 - -0.9 3,155,709 + +0.9 3,155,834 + DVU3031 0.16 -1.8 3,155,952 + DVU3031 0.26 +0.1 3,155,956 + DVU3031 0.27 -1.3 3,155,960 - DVU3031 0.27 -0.1 3,156,019 - DVU3031 0.33 -0.1 3,156,019 - DVU3031 0.33 +0.1 3,156,034 + DVU3031 0.34 +2.2 3,156,061 + DVU3031 0.36 -1.2 3,156,061 + DVU3031 0.36 +1.4 3,156,061 + DVU3031 0.36 -1.1 3,156,145 - DVU3031 0.44 +0.7 3,156,145 - DVU3031 0.44 -1.5 3,156,276 - DVU3031 0.56 +0.1 3,156,528 - DVU3031 0.79 -0.2 3,156,542 + DVU3031 0.80 -1.3 3,156,607 - DVU3031 0.86 -2.4 3,156,648 - -1.3 3,156,656 + -1.1 3,156,761 + +0.9 3,156,792 + -0.9 3,156,832 - DVU3032 0.13 -0.5 3,156,863 - DVU3032 0.18 -0.8 3,157,002 + DVU3032 0.40 -0.9 3,157,049 + DVU3032 0.47 -0.3 3,157,143 + DVU3032 0.62 -0.5 3,157,235 - DVU3032 0.77 -0.0 3,157,288 - DVU3032 0.85 +0.1 3,157,342 - -1.7 3,157,383 - -1.1 3,157,383 - +0.0 3,157,383 - -0.0 3,157,401 - +0.6 3,157,597 + -0.2 3,157,597 + -1.6 3,157,597 + -0.5 3,157,597 + -0.3 3,157,597 + -0.6 3,157,605 - DVU3033 0.10 -0.8 3,157,605 - DVU3033 0.10 -0.8 3,157,623 + DVU3033 0.11 -1.4 3,157,631 - DVU3033 0.11 -0.7
Or see this region's nucleotide sequence