Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DORF19125 and DVU2598 are separated by 96 nucleotides DVU2598 and DVU2599 overlap by 11 nucleotides DVU2599 and DVU2600 are separated by 140 nucleotides
DORF19125: DORF19125 - SEED: 12, at 2,718,809 to 2,719,957
DORF19125
DVU2598: DVU2598 - hypothetical protein (TIGR), at 2,720,054 to 2,720,608
DVU2598
DVU2599: DVU2599 - hypothetical protein (TIGR), at 2,720,598 to 2,721,041
DVU2599
DVU2600: DVU2600 - hypothetical protein (TIGR), at 2,721,182 to 2,721,583
DVU2600
Position (kb)
2720
2721 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2719.149 kb on + strand, within DORF19125 at 2719.212 kb on - strand, within DORF19125 at 2719.221 kb on + strand, within DORF19125 at 2719.297 kb on - strand, within DORF19125 at 2719.310 kb on + strand, within DORF19125 at 2719.311 kb on - strand, within DORF19125 at 2719.313 kb on - strand, within DORF19125 at 2719.379 kb on + strand, within DORF19125 at 2719.412 kb on - strand, within DORF19125 at 2719.428 kb on + strand, within DORF19125 at 2719.506 kb on + strand, within DORF19125 at 2719.537 kb on + strand, within DORF19125 at 2719.559 kb on - strand, within DORF19125 at 2719.608 kb on - strand, within DORF19125 at 2719.666 kb on - strand, within DORF19125 at 2719.686 kb on - strand, within DORF19125 at 2719.711 kb on - strand, within DORF19125 at 2719.742 kb on - strand, within DORF19125 at 2719.752 kb on + strand, within DORF19125 at 2719.768 kb on + strand, within DORF19125 at 2719.770 kb on - strand, within DORF19125 at 2719.776 kb on - strand, within DORF19125 at 2719.776 kb on - strand, within DORF19125 at 2719.860 kb on + strand at 2719.947 kb on + strand at 2719.947 kb on + strand at 2719.950 kb on - strand at 2719.976 kb on + strand at 2719.977 kb on + strand at 2720.026 kb on + strand at 2720.232 kb on + strand, within DVU2598 at 2720.321 kb on - strand, within DVU2598 at 2720.326 kb on - strand, within DVU2598 at 2720.383 kb on + strand, within DVU2598 at 2720.384 kb on - strand, within DVU2598 at 2720.420 kb on + strand, within DVU2598 at 2720.420 kb on + strand, within DVU2598 at 2720.428 kb on - strand, within DVU2598 at 2720.428 kb on - strand, within DVU2598 at 2720.515 kb on + strand, within DVU2598 at 2720.523 kb on - strand, within DVU2598 at 2720.558 kb on - strand at 2720.568 kb on - strand at 2720.570 kb on + strand at 2720.578 kb on - strand at 2720.610 kb on + strand at 2720.611 kb on + strand at 2720.693 kb on + strand, within DVU2599 at 2720.708 kb on + strand, within DVU2599 at 2720.717 kb on + strand, within DVU2599 at 2720.741 kb on + strand, within DVU2599 at 2720.749 kb on - strand, within DVU2599 at 2720.766 kb on - strand, within DVU2599 at 2720.847 kb on + strand, within DVU2599 at 2720.860 kb on - strand, within DVU2599 at 2720.934 kb on - strand, within DVU2599 at 2720.941 kb on + strand, within DVU2599 at 2721.026 kb on + strand at 2721.026 kb on + strand at 2721.026 kb on + strand at 2721.026 kb on + strand at 2721.034 kb on - strand at 2721.034 kb on - strand at 2721.034 kb on - strand at 2721.034 kb on - strand at 2721.085 kb on - strand at 2721.131 kb on - strand at 2721.134 kb on + strand at 2721.147 kb on - strand at 2721.163 kb on + strand at 2721.171 kb on - strand at 2721.171 kb on - strand at 2721.171 kb on - strand at 2721.195 kb on + strand at 2721.196 kb on - strand at 2721.208 kb on + strand at 2721.231 kb on + strand, within DVU2600 at 2721.351 kb on - strand, within DVU2600 at 2721.379 kb on + strand, within DVU2600 at 2721.462 kb on + strand, within DVU2600 at 2721.503 kb on + strand, within DVU2600 at 2721.503 kb on + strand, within DVU2600
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 2,719,149 + DORF19125 0.30 +0.3 2,719,212 - DORF19125 0.35 -0.1 2,719,221 + DORF19125 0.36 +0.1 2,719,297 - DORF19125 0.42 -0.3 2,719,310 + DORF19125 0.44 -0.6 2,719,311 - DORF19125 0.44 +0.1 2,719,313 - DORF19125 0.44 -0.1 2,719,379 + DORF19125 0.50 -0.8 2,719,412 - DORF19125 0.52 -0.2 2,719,428 + DORF19125 0.54 +0.3 2,719,506 + DORF19125 0.61 +0.1 2,719,537 + DORF19125 0.63 +0.9 2,719,559 - DORF19125 0.65 +1.5 2,719,608 - DORF19125 0.70 -0.0 2,719,666 - DORF19125 0.75 -1.2 2,719,686 - DORF19125 0.76 +0.0 2,719,711 - DORF19125 0.79 -0.8 2,719,742 - DORF19125 0.81 -0.6 2,719,752 + DORF19125 0.82 +0.2 2,719,768 + DORF19125 0.83 -0.5 2,719,770 - DORF19125 0.84 +0.0 2,719,776 - DORF19125 0.84 -0.0 2,719,776 - DORF19125 0.84 -0.0 2,719,860 + -0.1 2,719,947 + -0.1 2,719,947 + +0.0 2,719,950 - +0.1 2,719,976 + -0.1 2,719,977 + -0.2 2,720,026 + +0.4 2,720,232 + DVU2598 0.32 -0.9 2,720,321 - DVU2598 0.48 -0.4 2,720,326 - DVU2598 0.49 +0.4 2,720,383 + DVU2598 0.59 +1.1 2,720,384 - DVU2598 0.59 +0.2 2,720,420 + DVU2598 0.66 +1.2 2,720,420 + DVU2598 0.66 -1.0 2,720,428 - DVU2598 0.67 +0.1 2,720,428 - DVU2598 0.67 +0.6 2,720,515 + DVU2598 0.83 -1.3 2,720,523 - DVU2598 0.85 -0.1 2,720,558 - +0.4 2,720,568 - -2.8 2,720,570 + +0.3 2,720,578 - -1.0 2,720,610 + -0.3 2,720,611 + +0.3 2,720,693 + DVU2599 0.21 -0.3 2,720,708 + DVU2599 0.25 -0.2 2,720,717 + DVU2599 0.27 -0.8 2,720,741 + DVU2599 0.32 -0.1 2,720,749 - DVU2599 0.34 +0.9 2,720,766 - DVU2599 0.38 -0.0 2,720,847 + DVU2599 0.56 -0.3 2,720,860 - DVU2599 0.59 -0.2 2,720,934 - DVU2599 0.76 +0.1 2,720,941 + DVU2599 0.77 -2.1 2,721,026 + -0.4 2,721,026 + -0.1 2,721,026 + -0.3 2,721,026 + -1.5 2,721,034 - -0.0 2,721,034 - +0.8 2,721,034 - +1.1 2,721,034 - -0.2 2,721,085 - -1.0 2,721,131 - -0.1 2,721,134 + +0.0 2,721,147 - +0.8 2,721,163 + +0.7 2,721,171 - -1.2 2,721,171 - -1.0 2,721,171 - -0.3 2,721,195 + +0.8 2,721,196 - -0.8 2,721,208 + +0.1 2,721,231 + DVU2600 0.12 +0.1 2,721,351 - DVU2600 0.42 -0.7 2,721,379 + DVU2600 0.49 +0.1 2,721,462 + DVU2600 0.70 +0.4 2,721,503 + DVU2600 0.80 +0.2 2,721,503 + DVU2600 0.80 +0.1
Or see this region's nucleotide sequence