Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2287 and DVU2288 are separated by 12 nucleotides DVU2288 and b2488 are separated by 1 nucleotides b2488 and DVU2290 overlap by 4 nucleotides DVU2290 and DVU2291 overlap by 4 nucleotides
DVU2287: DVU2287 - hydrogenase, CooK subunit, selenocysteine-containing, putative (TIGR), at 2,381,054 to 2,382,019
DVU2287
DVU2288: DVU2288 - hydrogenase, CooL subunit, putative (TIGR), at 2,382,032 to 2,382,466
DVU2288
DVU2289: b2488 - hydrogenase, CooX subunit, putative (TIGR), at 2,382,468 to 2,383,103
b2488
DVU2290: DVU2290 - hydrogenase, CooU subunit, putative (TIGR), at 2,383,100 to 2,383,645
DVU2290
DVU2291: DVU2291 - carbon monoxide-induced hydrogenase CooH, putative (TIGR), at 2,383,642 to 2,384,742
DVU2291
Position (kb)
2382
2383
2384 Strain fitness (log2 ratio)
-2
-1
0
1 at 2381.490 kb on - strand, within DVU2287 at 2381.605 kb on + strand, within DVU2287 at 2382.243 kb on - strand, within DVU2288 at 2382.750 kb on - strand, within b2488 at 2382.926 kb on + strand, within b2488 at 2383.041 kb on + strand at 2383.041 kb on + strand at 2383.041 kb on + strand at 2383.116 kb on + strand at 2383.243 kb on + strand, within DVU2290 at 2383.365 kb on + strand, within DVU2290 at 2383.639 kb on + strand at 2383.775 kb on + strand, within DVU2291 at 2383.819 kb on - strand, within DVU2291 at 2384.062 kb on + strand, within DVU2291
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 2,381,490 - DVU2287 0.45 +0.8 2,381,605 + DVU2287 0.57 -0.2 2,382,243 - DVU2288 0.49 -1.1 2,382,750 - b2488 DVU2289 0.44 -0.1 2,382,926 + b2488 DVU2289 0.72 -0.4 2,383,041 + +0.0 2,383,041 + -0.4 2,383,041 + -2.5 2,383,116 + -1.1 2,383,243 + DVU2290 0.26 -1.2 2,383,365 + DVU2290 0.49 +0.5 2,383,639 + -0.4 2,383,775 + DVU2291 0.12 -1.5 2,383,819 - DVU2291 0.16 +0.3 2,384,062 + DVU2291 0.38 -0.8
Or see this region's nucleotide sequence