Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2213 and DVU2214 are separated by 78 nucleotides DVU2214 and DVU2215 are separated by 152 nucleotides DVU2215 and infA-2 are separated by 79 nucleotides infA-2 and DVU2217 are separated by 534 nucleotides
DVU2213: DVU2213 - nuclease domain protein (TIGR), at 2,310,270 to 2,310,971
DVU2213
DVU2214: DVU2214 - hypothetical protein (TIGR), at 2,311,050 to 2,311,223
DVU2214
DVU2215: DVU2215 - RNA-binding protein (TIGR), at 2,311,376 to 2,311,645
DVU2215
DVU2216: infA-2 - translation initiation factor IF-1 (TIGR), at 2,311,725 to 2,311,988
infA-2
DVU2217: DVU2217 - acetyltransferase, GNAT family (TIGR), at 2,312,523 to 2,313,029
DVU2217
Position (kb)
2311
2312 Strain fitness (log2 ratio)
-2
-1
0
1 at 2310.725 kb on + strand, within DVU2213 at 2310.740 kb on - strand, within DVU2213 at 2310.781 kb on - strand, within DVU2213 at 2310.786 kb on - strand, within DVU2213 at 2310.817 kb on + strand, within DVU2213 at 2310.824 kb on + strand, within DVU2213 at 2310.824 kb on + strand, within DVU2213 at 2310.824 kb on + strand, within DVU2213 at 2310.834 kb on - strand, within DVU2213 at 2310.905 kb on + strand at 2310.910 kb on + strand at 2310.918 kb on - strand at 2310.923 kb on - strand at 2310.925 kb on - strand at 2310.938 kb on - strand at 2310.941 kb on + strand at 2310.993 kb on + strand at 2311.083 kb on - strand, within DVU2214 at 2311.106 kb on - strand, within DVU2214 at 2311.133 kb on + strand, within DVU2214 at 2311.156 kb on - strand, within DVU2214 at 2311.168 kb on - strand, within DVU2214 at 2311.887 kb on + strand, within infA-2 at 2311.887 kb on + strand, within infA-2 at 2311.929 kb on - strand, within infA-2 at 2311.936 kb on + strand, within infA-2 at 2311.997 kb on + strand at 2312.081 kb on - strand at 2312.125 kb on + strand at 2312.312 kb on - strand at 2312.528 kb on - strand at 2312.754 kb on - strand, within DVU2217 at 2312.764 kb on - strand, within DVU2217 at 2312.791 kb on + strand, within DVU2217 at 2312.794 kb on - strand, within DVU2217 at 2312.794 kb on - strand, within DVU2217 at 2312.806 kb on - strand, within DVU2217 at 2312.814 kb on - strand, within DVU2217 at 2312.906 kb on - strand, within DVU2217 at 2312.921 kb on - strand, within DVU2217
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 2,310,725 + DVU2213 0.65 -1.2 2,310,740 - DVU2213 0.67 -1.8 2,310,781 - DVU2213 0.73 +0.1 2,310,786 - DVU2213 0.74 -0.2 2,310,817 + DVU2213 0.78 +0.0 2,310,824 + DVU2213 0.79 -0.4 2,310,824 + DVU2213 0.79 -0.5 2,310,824 + DVU2213 0.79 -0.2 2,310,834 - DVU2213 0.80 -1.0 2,310,905 + -0.0 2,310,910 + -0.3 2,310,918 - -0.0 2,310,923 - -0.0 2,310,925 - +0.3 2,310,938 - -0.7 2,310,941 + +0.1 2,310,993 + -0.5 2,311,083 - DVU2214 0.19 -2.5 2,311,106 - DVU2214 0.32 -0.7 2,311,133 + DVU2214 0.48 -0.1 2,311,156 - DVU2214 0.61 -1.2 2,311,168 - DVU2214 0.68 +0.4 2,311,887 + infA-2 DVU2216 0.61 -1.0 2,311,887 + infA-2 DVU2216 0.61 -0.5 2,311,929 - infA-2 DVU2216 0.77 -1.0 2,311,936 + infA-2 DVU2216 0.80 -0.1 2,311,997 + -0.0 2,312,081 - -0.2 2,312,125 + +0.1 2,312,312 - +0.6 2,312,528 - -0.0 2,312,754 - DVU2217 0.46 -0.0 2,312,764 - DVU2217 0.48 -0.2 2,312,791 + DVU2217 0.53 -1.6 2,312,794 - DVU2217 0.53 +0.1 2,312,794 - DVU2217 0.53 +0.2 2,312,806 - DVU2217 0.56 +0.2 2,312,814 - DVU2217 0.57 -1.7 2,312,906 - DVU2217 0.76 +0.4 2,312,921 - DVU2217 0.79 -1.0
Or see this region's nucleotide sequence