Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trmE and DVU1080 are separated by 318 nucleotides DVU1080 and DVU1081 overlap by 4 nucleotides
DVU1079: trmE - tRNA modification GTPase TrmE (TIGR), at 1,182,378 to 1,183,751
trmE
DVU1080: DVU1080 - iron-sulfur cluster-binding protein (TIGR), at 1,184,070 to 1,184,612
DVU1080
DVU1081: DVU1081 - iron-sulfur cluster-binding protein (TIGR), at 1,184,609 to 1,186,228
DVU1081
Position (kb)
1184
1185 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1183.097 kb on + strand, within trmE at 1183.097 kb on + strand, within trmE at 1183.161 kb on + strand, within trmE at 1183.209 kb on - strand, within trmE at 1183.516 kb on + strand, within trmE at 1183.574 kb on + strand, within trmE at 1183.679 kb on - strand at 1183.949 kb on - strand at 1183.949 kb on - strand at 1184.102 kb on - strand at 1184.212 kb on + strand, within DVU1080 at 1184.218 kb on - strand, within DVU1080 at 1184.243 kb on + strand, within DVU1080 at 1184.583 kb on + strand at 1184.597 kb on + strand at 1184.597 kb on + strand at 1184.605 kb on + strand at 1184.605 kb on - strand at 1184.605 kb on - strand at 1184.605 kb on - strand at 1184.605 kb on - strand at 1184.605 kb on - strand at 1184.681 kb on - strand at 1184.691 kb on - strand at 1184.725 kb on + strand at 1184.725 kb on + strand at 1184.733 kb on - strand at 1184.759 kb on + strand at 1184.888 kb on + strand, within DVU1081 at 1184.888 kb on + strand, within DVU1081 at 1184.891 kb on - strand, within DVU1081 at 1184.967 kb on + strand, within DVU1081 at 1184.975 kb on - strand, within DVU1081 at 1185.001 kb on - strand, within DVU1081 at 1185.030 kb on - strand, within DVU1081 at 1185.046 kb on - strand, within DVU1081 at 1185.057 kb on + strand, within DVU1081 at 1185.070 kb on + strand, within DVU1081 at 1185.166 kb on - strand, within DVU1081 at 1185.178 kb on + strand, within DVU1081 at 1185.199 kb on - strand, within DVU1081 at 1185.230 kb on - strand, within DVU1081 at 1185.245 kb on + strand, within DVU1081 at 1185.250 kb on + strand, within DVU1081 at 1185.258 kb on - strand, within DVU1081 at 1185.319 kb on + strand, within DVU1081 at 1185.348 kb on - strand, within DVU1081 at 1185.355 kb on + strand, within DVU1081 at 1185.392 kb on + strand, within DVU1081 at 1185.576 kb on + strand, within DVU1081 at 1185.591 kb on + strand, within DVU1081
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 1,183,097 + trmE DVU1079 0.52 -0.1 1,183,097 + trmE DVU1079 0.52 -0.7 1,183,161 + trmE DVU1079 0.57 +0.2 1,183,209 - trmE DVU1079 0.60 -0.7 1,183,516 + trmE DVU1079 0.83 +0.2 1,183,574 + trmE DVU1079 0.87 -0.2 1,183,679 - -1.1 1,183,949 - +0.1 1,183,949 - +0.2 1,184,102 - -0.3 1,184,212 + DVU1080 0.26 -1.3 1,184,218 - DVU1080 0.27 -0.1 1,184,243 + DVU1080 0.32 -2.0 1,184,583 + -0.6 1,184,597 + +0.1 1,184,597 + -0.4 1,184,605 + +0.1 1,184,605 - -0.0 1,184,605 - -1.4 1,184,605 - +0.5 1,184,605 - +0.3 1,184,605 - +0.1 1,184,681 - +0.1 1,184,691 - -0.6 1,184,725 + -0.0 1,184,725 + +0.1 1,184,733 - -0.1 1,184,759 + -0.2 1,184,888 + DVU1081 0.17 +1.0 1,184,888 + DVU1081 0.17 -0.1 1,184,891 - DVU1081 0.17 +0.9 1,184,967 + DVU1081 0.22 -1.1 1,184,975 - DVU1081 0.23 -0.0 1,185,001 - DVU1081 0.24 +0.3 1,185,030 - DVU1081 0.26 -0.3 1,185,046 - DVU1081 0.27 -1.5 1,185,057 + DVU1081 0.28 +1.2 1,185,070 + DVU1081 0.28 -0.6 1,185,166 - DVU1081 0.34 -0.1 1,185,178 + DVU1081 0.35 +0.0 1,185,199 - DVU1081 0.36 -1.0 1,185,230 - DVU1081 0.38 -0.0 1,185,245 + DVU1081 0.39 +0.2 1,185,250 + DVU1081 0.40 -0.2 1,185,258 - DVU1081 0.40 -0.1 1,185,319 + DVU1081 0.44 -1.0 1,185,348 - DVU1081 0.46 +0.1 1,185,355 + DVU1081 0.46 -0.3 1,185,392 + DVU1081 0.48 +1.8 1,185,576 + DVU1081 0.60 -0.5 1,185,591 + DVU1081 0.61 -0.6
Or see this region's nucleotide sequence