Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0827 and smpB are separated by 28 nucleotides smpB and ptsI are separated by 11 nucleotides ptsI and ptsH are separated by 10 nucleotides ptsH and DVU0831 are separated by 60 nucleotides
DVU0827: DVU0827 - glycolate oxidase, subunit GlcD, putative (TIGR), at 917,179 to 918,594
DVU0827
DVU0828: smpB - SsrA-binding protein (TIGR), at 918,623 to 919,087
smpB
DVU0829: ptsI - phosphoenolpyruvate-protein phosphotransferase (TIGR), at 919,099 to 920,871
ptsI
DVU0830: ptsH - phosphocarrier protein HPr (TIGR), at 920,882 to 921,142
ptsH
DVU0831: DVU0831 - PTS system, IID component, putative (TIGR), at 921,203 to 921,961
DVU0831
Position (kb) 919
920
921 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 918.199 kb on + strand, within DVU0827 at 918.561 kb on - strand at 918.592 kb on - strand at 918.602 kb on + strand at 918.602 kb on + strand at 918.602 kb on + strand at 918.610 kb on - strand at 918.610 kb on - strand at 918.620 kb on - strand at 918.621 kb on + strand at 919.172 kb on + strand at 919.194 kb on - strand at 919.202 kb on + strand at 919.212 kb on + strand at 919.212 kb on + strand at 919.244 kb on - strand at 919.407 kb on + strand, within ptsI at 919.440 kb on - strand, within ptsI at 919.555 kb on + strand, within ptsI at 919.583 kb on + strand, within ptsI at 919.600 kb on - strand, within ptsI at 919.747 kb on - strand, within ptsI at 919.808 kb on + strand, within ptsI at 919.808 kb on + strand, within ptsI at 919.816 kb on - strand, within ptsI at 919.986 kb on + strand, within ptsI at 919.986 kb on + strand, within ptsI at 919.989 kb on - strand, within ptsI at 919.989 kb on - strand, within ptsI at 919.989 kb on - strand, within ptsI at 919.989 kb on - strand, within ptsI at 919.989 kb on - strand, within ptsI at 920.050 kb on - strand, within ptsI at 920.141 kb on + strand, within ptsI at 920.314 kb on - strand, within ptsI at 921.108 kb on - strand, within ptsH at 921.231 kb on - strand at 921.384 kb on + strand, within DVU0831 at 921.384 kb on + strand, within DVU0831 at 921.428 kb on - strand, within DVU0831 at 921.475 kb on + strand, within DVU0831 at 921.482 kb on - strand, within DVU0831 at 921.493 kb on + strand, within DVU0831 at 921.557 kb on - strand, within DVU0831 at 921.583 kb on + strand, within DVU0831 at 921.594 kb on + strand, within DVU0831 at 921.708 kb on - strand, within DVU0831 at 921.819 kb on + strand, within DVU0831
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 918,199 + DVU0827 0.72 +0.4 918,561 - +0.1 918,592 - -0.3 918,602 + +0.3 918,602 + +0.2 918,602 + +0.6 918,610 - -0.2 918,610 - -1.3 918,620 - +0.3 918,621 + +0.0 919,172 + -0.2 919,194 - -0.3 919,202 + -0.1 919,212 + +0.2 919,212 + -0.6 919,244 - +0.2 919,407 + ptsI DVU0829 0.17 -0.1 919,440 - ptsI DVU0829 0.19 +0.1 919,555 + ptsI DVU0829 0.26 -1.9 919,583 + ptsI DVU0829 0.27 -1.6 919,600 - ptsI DVU0829 0.28 -0.1 919,747 - ptsI DVU0829 0.37 +1.0 919,808 + ptsI DVU0829 0.40 -4.0 919,808 + ptsI DVU0829 0.40 -0.5 919,816 - ptsI DVU0829 0.40 +0.0 919,986 + ptsI DVU0829 0.50 -2.7 919,986 + ptsI DVU0829 0.50 -0.9 919,989 - ptsI DVU0829 0.50 -0.5 919,989 - ptsI DVU0829 0.50 -0.9 919,989 - ptsI DVU0829 0.50 -1.2 919,989 - ptsI DVU0829 0.50 -0.7 919,989 - ptsI DVU0829 0.50 -1.2 920,050 - ptsI DVU0829 0.54 +0.2 920,141 + ptsI DVU0829 0.59 -1.3 920,314 - ptsI DVU0829 0.69 -1.0 921,108 - ptsH DVU0830 0.87 +0.2 921,231 - -0.3 921,384 + DVU0831 0.24 -3.1 921,384 + DVU0831 0.24 -0.8 921,428 - DVU0831 0.30 -1.2 921,475 + DVU0831 0.36 -1.4 921,482 - DVU0831 0.37 -1.7 921,493 + DVU0831 0.38 -0.3 921,557 - DVU0831 0.47 -1.4 921,583 + DVU0831 0.50 +0.4 921,594 + DVU0831 0.52 -0.3 921,708 - DVU0831 0.67 -1.5 921,819 + DVU0831 0.81 -1.6
Or see this region's nucleotide sequence