Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0645 and cobI are separated by 209 nucleotides cobI and DVU0647 are separated by 1 nucleotides DVU0647 and fepC overlap by 23 nucleotides fepC and DVU0649 overlap by 4 nucleotides
DVU0645: DVU0645 - methyl-accepting chemotaxis protein (TIGR), at 714,819 to 716,858
DVU0645
DVU0646: cobI - precorrin-2 C20-methyltransferase (TIGR), at 717,068 to 717,838
cobI
DVU0647: DVU0647 - iron compound ABC transporter, periplasmic iron compount-binding protein, putative (TIGR), at 717,840 to 718,754
DVU0647
DVU0648: fepC - iron compound ABC transporter, ATP-binding protein (TIGR), at 718,732 to 719,547
fepC
DVU0649: DVU0649 - iron compound ABC transporter, permease protein (TIGR), at 719,544 to 720,599
DVU0649
Position (kb) 717
718
719 Strain fitness (log2 ratio) -2
-1
0
1 at 716.842 kb on + strand at 716.860 kb on - strand at 716.860 kb on - strand at 716.909 kb on - strand at 716.998 kb on + strand at 717.022 kb on + strand at 717.073 kb on - strand at 717.157 kb on + strand, within cobI at 717.165 kb on - strand, within cobI at 717.264 kb on - strand, within cobI at 717.342 kb on + strand, within cobI at 717.406 kb on + strand, within cobI at 717.414 kb on - strand, within cobI at 717.480 kb on - strand, within cobI at 717.519 kb on + strand, within cobI at 717.628 kb on + strand, within cobI at 717.687 kb on - strand, within cobI at 717.711 kb on - strand, within cobI at 717.758 kb on - strand, within cobI at 717.761 kb on + strand, within cobI at 717.763 kb on - strand at 717.769 kb on - strand at 717.925 kb on + strand at 717.977 kb on - strand, within DVU0647 at 718.134 kb on - strand, within DVU0647 at 718.134 kb on - strand, within DVU0647 at 718.134 kb on - strand, within DVU0647 at 718.297 kb on + strand, within DVU0647 at 718.307 kb on - strand, within DVU0647 at 718.381 kb on + strand, within DVU0647 at 718.405 kb on + strand, within DVU0647 at 718.413 kb on - strand, within DVU0647 at 718.413 kb on - strand, within DVU0647 at 718.516 kb on + strand, within DVU0647 at 718.542 kb on + strand, within DVU0647 at 718.611 kb on + strand, within DVU0647 at 718.711 kb on + strand at 718.737 kb on - strand at 718.744 kb on + strand at 718.746 kb on + strand at 718.746 kb on + strand at 718.754 kb on - strand at 718.754 kb on - strand at 718.779 kb on + strand at 718.782 kb on - strand at 718.871 kb on + strand, within fepC at 718.889 kb on + strand, within fepC at 718.912 kb on - strand, within fepC at 718.934 kb on + strand, within fepC at 718.939 kb on + strand, within fepC at 718.947 kb on - strand, within fepC at 718.947 kb on - strand, within fepC at 719.020 kb on + strand, within fepC at 719.021 kb on - strand, within fepC at 719.054 kb on - strand, within fepC at 719.145 kb on - strand, within fepC at 719.265 kb on + strand, within fepC at 719.439 kb on + strand, within fepC at 719.630 kb on + strand at 719.630 kb on + strand at 719.638 kb on - strand at 719.638 kb on - strand at 719.734 kb on - strand, within DVU0649
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 716,842 + -0.3 716,860 - -1.7 716,860 - +0.4 716,909 - -0.1 716,998 + -1.0 717,022 + +0.8 717,073 - +0.7 717,157 + cobI DVU0646 0.12 +0.3 717,165 - cobI DVU0646 0.13 -0.3 717,264 - cobI DVU0646 0.25 -0.1 717,342 + cobI DVU0646 0.36 +0.1 717,406 + cobI DVU0646 0.44 +0.3 717,414 - cobI DVU0646 0.45 +0.3 717,480 - cobI DVU0646 0.53 -1.2 717,519 + cobI DVU0646 0.58 -0.2 717,628 + cobI DVU0646 0.73 +0.1 717,687 - cobI DVU0646 0.80 -0.1 717,711 - cobI DVU0646 0.83 -1.1 717,758 - cobI DVU0646 0.89 -0.9 717,761 + cobI DVU0646 0.90 -0.1 717,763 - +0.1 717,769 - -0.0 717,925 + -1.7 717,977 - DVU0647 0.15 -1.0 718,134 - DVU0647 0.32 -0.2 718,134 - DVU0647 0.32 -1.3 718,134 - DVU0647 0.32 -1.4 718,297 + DVU0647 0.50 -0.2 718,307 - DVU0647 0.51 -0.5 718,381 + DVU0647 0.59 -0.9 718,405 + DVU0647 0.62 -0.1 718,413 - DVU0647 0.63 -0.6 718,413 - DVU0647 0.63 +0.3 718,516 + DVU0647 0.74 -0.4 718,542 + DVU0647 0.77 -1.9 718,611 + DVU0647 0.84 -0.2 718,711 + -0.6 718,737 - -0.0 718,744 + -0.5 718,746 + -0.0 718,746 + -0.2 718,754 - -0.4 718,754 - -1.7 718,779 + +0.5 718,782 - -0.6 718,871 + fepC DVU0648 0.17 -1.2 718,889 + fepC DVU0648 0.19 +0.3 718,912 - fepC DVU0648 0.22 -0.0 718,934 + fepC DVU0648 0.25 +1.3 718,939 + fepC DVU0648 0.25 -0.9 718,947 - fepC DVU0648 0.26 +0.2 718,947 - fepC DVU0648 0.26 +0.2 719,020 + fepC DVU0648 0.35 -1.9 719,021 - fepC DVU0648 0.35 -0.8 719,054 - fepC DVU0648 0.39 -0.4 719,145 - fepC DVU0648 0.51 +0.2 719,265 + fepC DVU0648 0.65 +0.0 719,439 + fepC DVU0648 0.87 -0.5 719,630 + -0.5 719,630 + -0.1 719,638 - +0.0 719,638 - -1.1 719,734 - DVU0649 0.18 +0.6
Or see this region's nucleotide sequence