Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt escJ and DORF2116 are separated by 6 nucleotides DORF2116 and DVUA0118 overlap by 22 nucleotides DVUA0118 and sctN are separated by 327 nucleotides
DVUA0117: escJ - type III secretion lipoprotein (TIGR), at 154,970 to 155,785
escJ
DORF2116: DORF2116 - SEED: FIG00606188: hypothetical protein, at 155,792 to 156,715
DORF2116
DVUA0118: DVUA0118 - type III secretion protein, YopL family (TIGR), at 156,694 to 157,311
DVUA0118
DVUA0119: sctN - type III secretion system ATPase (TIGR), at 157,639 to 158,955
sctN
Position (kb) 156
157
158 Strain fitness (log2 ratio) -1
0
1 at 155.765 kb on + strand at 155.775 kb on + strand at 155.780 kb on + strand at 155.839 kb on + strand at 155.839 kb on + strand at 155.847 kb on - strand at 156.146 kb on + strand, within DORF2116 at 156.160 kb on + strand, within DORF2116 at 156.206 kb on - strand, within DORF2116 at 156.231 kb on + strand, within DORF2116 at 156.231 kb on + strand, within DORF2116 at 156.235 kb on + strand, within DORF2116 at 156.236 kb on + strand, within DORF2116 at 156.254 kb on + strand, within DORF2116 at 156.325 kb on - strand, within DORF2116 at 156.339 kb on - strand, within DORF2116 at 156.344 kb on + strand, within DORF2116 at 156.419 kb on + strand, within DORF2116 at 156.419 kb on + strand, within DORF2116 at 156.574 kb on + strand, within DORF2116 at 156.608 kb on - strand, within DORF2116 at 156.713 kb on - strand at 156.887 kb on + strand, within DVUA0118 at 156.895 kb on - strand, within DVUA0118 at 156.922 kb on + strand, within DVUA0118 at 156.934 kb on + strand, within DVUA0118 at 156.949 kb on - strand, within DVUA0118 at 157.445 kb on - strand at 157.459 kb on + strand at 157.474 kb on + strand at 157.482 kb on - strand at 157.482 kb on - strand at 157.562 kb on - strand at 157.607 kb on + strand at 157.665 kb on - strand at 157.840 kb on + strand, within sctN at 157.863 kb on + strand, within sctN at 157.870 kb on + strand, within sctN at 157.896 kb on + strand, within sctN at 157.904 kb on - strand, within sctN at 158.034 kb on + strand, within sctN at 158.042 kb on - strand, within sctN at 158.066 kb on + strand, within sctN at 158.092 kb on + strand, within sctN at 158.109 kb on - strand, within sctN at 158.119 kb on + strand, within sctN at 158.242 kb on - strand, within sctN at 158.242 kb on - strand, within sctN at 158.270 kb on + strand, within sctN at 158.304 kb on + strand, within sctN
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 155,765 + -0.4 155,775 + -0.3 155,780 + -0.7 155,839 + -0.9 155,839 + -0.1 155,847 - -0.7 156,146 + DORF2116 0.38 -0.1 156,160 + DORF2116 0.40 -1.5 156,206 - DORF2116 0.45 +0.0 156,231 + DORF2116 0.48 -0.7 156,231 + DORF2116 0.48 -0.5 156,235 + DORF2116 0.48 +0.2 156,236 + DORF2116 0.48 -0.2 156,254 + DORF2116 0.50 +0.2 156,325 - DORF2116 0.58 -0.2 156,339 - DORF2116 0.59 -0.4 156,344 + DORF2116 0.60 -0.8 156,419 + DORF2116 0.68 +0.1 156,419 + DORF2116 0.68 -0.2 156,574 + DORF2116 0.85 +1.2 156,608 - DORF2116 0.88 +0.2 156,713 - +0.1 156,887 + DVUA0118 0.31 -1.0 156,895 - DVUA0118 0.33 +0.2 156,922 + DVUA0118 0.37 -0.7 156,934 + DVUA0118 0.39 +0.2 156,949 - DVUA0118 0.41 -0.9 157,445 - -0.0 157,459 + +0.1 157,474 + +0.1 157,482 - +0.2 157,482 - -1.5 157,562 - -0.2 157,607 + +0.4 157,665 - -0.7 157,840 + sctN DVUA0119 0.15 +0.6 157,863 + sctN DVUA0119 0.17 -0.8 157,870 + sctN DVUA0119 0.18 +0.5 157,896 + sctN DVUA0119 0.20 -0.6 157,904 - sctN DVUA0119 0.20 +0.2 158,034 + sctN DVUA0119 0.30 -0.7 158,042 - sctN DVUA0119 0.31 +0.1 158,066 + sctN DVUA0119 0.32 -0.3 158,092 + sctN DVUA0119 0.34 +0.1 158,109 - sctN DVUA0119 0.36 +0.1 158,119 + sctN DVUA0119 0.36 +0.4 158,242 - sctN DVUA0119 0.46 -0.2 158,242 - sctN DVUA0119 0.46 -0.2 158,270 + sctN DVUA0119 0.48 +0.2 158,304 + sctN DVUA0119 0.50 +0.1
Or see this region's nucleotide sequence