Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2510 and DVU2511 are separated by 14 nucleotides DVU2511 and mraW are separated by 2 nucleotides mraW and mraZ are separated by 13 nucleotides mraZ and pyk are separated by 153 nucleotides
DVU2510: DVU2510 - penicillin-binding protein (TIGR), at 2,623,154 to 2,625,226
DVU2510
DVU2511: DVU2511 - hypothetical protein (TIGR), at 2,625,241 to 2,625,528
DVU2511
DVU2512: mraW - S-adenosyl-methyltransferase MraW (TIGR), at 2,625,531 to 2,626,502
mraW
DVU2513: mraZ - conserved hypothetical protein TIGR00242 (TIGR), at 2,626,516 to 2,626,965
mraZ
DVU2514: pyk - pyruvate kinase (TIGR), at 2,627,119 to 2,628,528
pyk
Position (kb)
2625
2626
2627 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2625.134 kb on - strand at 2625.228 kb on + strand at 2625.528 kb on + strand at 2625.536 kb on - strand at 2625.536 kb on - strand at 2625.536 kb on - strand at 2626.001 kb on - strand, within mraW at 2626.211 kb on - strand, within mraW at 2626.215 kb on - strand, within mraW at 2626.333 kb on - strand, within mraW at 2626.568 kb on - strand, within mraZ at 2626.641 kb on - strand, within mraZ at 2626.738 kb on - strand, within mraZ at 2626.741 kb on - strand, within mraZ at 2626.777 kb on - strand, within mraZ at 2626.810 kb on + strand, within mraZ at 2626.882 kb on - strand, within mraZ at 2627.037 kb on + strand at 2627.037 kb on + strand at 2627.042 kb on + strand at 2627.045 kb on - strand at 2627.253 kb on + strand at 2627.261 kb on - strand, within pyk at 2627.285 kb on - strand, within pyk at 2627.314 kb on - strand, within pyk at 2627.381 kb on - strand, within pyk at 2627.382 kb on - strand, within pyk at 2627.382 kb on - strand, within pyk at 2627.432 kb on + strand, within pyk at 2627.432 kb on + strand, within pyk at 2627.477 kb on + strand, within pyk
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 2,625,134 - +0.7 2,625,228 + +0.5 2,625,528 + +1.7 2,625,536 - +0.1 2,625,536 - +0.4 2,625,536 - +0.3 2,626,001 - mraW DVU2512 0.48 +0.3 2,626,211 - mraW DVU2512 0.70 -0.3 2,626,215 - mraW DVU2512 0.70 +0.7 2,626,333 - mraW DVU2512 0.83 +0.6 2,626,568 - mraZ DVU2513 0.12 -0.7 2,626,641 - mraZ DVU2513 0.28 -1.7 2,626,738 - mraZ DVU2513 0.49 +0.3 2,626,741 - mraZ DVU2513 0.50 +0.5 2,626,777 - mraZ DVU2513 0.58 -0.1 2,626,810 + mraZ DVU2513 0.65 -0.6 2,626,882 - mraZ DVU2513 0.81 -0.5 2,627,037 + -0.2 2,627,037 + +0.5 2,627,042 + -0.8 2,627,045 - -0.0 2,627,253 + -0.9 2,627,261 - pyk DVU2514 0.10 -1.2 2,627,285 - pyk DVU2514 0.12 -0.0 2,627,314 - pyk DVU2514 0.14 -1.3 2,627,381 - pyk DVU2514 0.19 -1.8 2,627,382 - pyk DVU2514 0.19 -3.0 2,627,382 - pyk DVU2514 0.19 -1.5 2,627,432 + pyk DVU2514 0.22 -0.9 2,627,432 + pyk DVU2514 0.22 -1.4 2,627,477 + pyk DVU2514 0.25 -1.8
Or see this region's nucleotide sequence