Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2135 and DVU2136 are separated by 77 nucleotides DVU2136 and sucCD are separated by 220 nucleotides
DVU2135: DVU2135 - hypothetical protein (TIGR), at 2,231,340 to 2,231,891
DVU2135
DVU2136: DVU2136 - hypothetical protein (TIGR), at 2,231,969 to 2,233,150
DVU2136
DVU2137: sucCD - succinyl-CoA synthetase, alpha/beta subunit (sucCD) (Chris Hemme), at 2,233,371 to 2,235,500
sucCD
Position (kb)
2231
2232
2233
2234 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2231.007 kb on + strand at 2231.032 kb on + strand at 2231.032 kb on + strand at 2231.064 kb on - strand at 2231.072 kb on - strand at 2231.194 kb on - strand at 2231.243 kb on + strand at 2231.324 kb on + strand at 2231.383 kb on - strand at 2231.437 kb on + strand, within DVU2135 at 2231.444 kb on + strand, within DVU2135 at 2231.444 kb on + strand, within DVU2135 at 2231.452 kb on - strand, within DVU2135 at 2231.556 kb on + strand, within DVU2135 at 2231.583 kb on - strand, within DVU2135 at 2231.599 kb on - strand, within DVU2135 at 2231.605 kb on - strand, within DVU2135 at 2231.736 kb on + strand, within DVU2135 at 2231.736 kb on + strand, within DVU2135 at 2231.871 kb on - strand at 2231.890 kb on + strand at 2231.923 kb on + strand at 2231.947 kb on + strand at 2232.057 kb on + strand at 2232.064 kb on + strand at 2232.133 kb on - strand, within DVU2136 at 2232.181 kb on - strand, within DVU2136 at 2232.245 kb on + strand, within DVU2136 at 2232.411 kb on + strand, within DVU2136 at 2232.411 kb on + strand, within DVU2136 at 2232.419 kb on - strand, within DVU2136 at 2232.446 kb on - strand, within DVU2136 at 2232.564 kb on + strand, within DVU2136 at 2232.572 kb on - strand, within DVU2136 at 2232.572 kb on - strand, within DVU2136 at 2232.572 kb on - strand, within DVU2136 at 2232.579 kb on + strand, within DVU2136 at 2232.587 kb on - strand, within DVU2136 at 2232.642 kb on + strand, within DVU2136 at 2232.650 kb on + strand, within DVU2136 at 2232.668 kb on - strand, within DVU2136 at 2232.730 kb on - strand, within DVU2136 at 2232.744 kb on - strand, within DVU2136 at 2232.746 kb on + strand, within DVU2136 at 2232.770 kb on + strand, within DVU2136 at 2232.841 kb on - strand, within DVU2136 at 2232.866 kb on + strand, within DVU2136 at 2232.881 kb on - strand, within DVU2136 at 2232.889 kb on + strand, within DVU2136 at 2232.895 kb on - strand, within DVU2136 at 2232.897 kb on - strand, within DVU2136 at 2232.929 kb on + strand, within DVU2136 at 2232.935 kb on + strand, within DVU2136 at 2233.035 kb on + strand at 2233.053 kb on - strand at 2233.092 kb on - strand at 2233.118 kb on + strand at 2233.222 kb on + strand at 2233.237 kb on - strand at 2233.327 kb on + strand at 2233.382 kb on - strand at 2233.502 kb on + strand at 2233.510 kb on - strand at 2233.663 kb on + strand, within sucCD at 2233.739 kb on - strand, within sucCD at 2233.935 kb on + strand, within sucCD at 2233.936 kb on - strand, within sucCD at 2233.954 kb on + strand, within sucCD at 2234.087 kb on - strand, within sucCD at 2234.087 kb on - strand, within sucCD at 2234.113 kb on + strand, within sucCD at 2234.121 kb on - strand, within sucCD
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 2,231,007 + -0.5 2,231,032 + -0.2 2,231,032 + -0.2 2,231,064 - -2.7 2,231,072 - -1.5 2,231,194 - -1.8 2,231,243 + -0.5 2,231,324 + -0.7 2,231,383 - +0.2 2,231,437 + DVU2135 0.18 -0.0 2,231,444 + DVU2135 0.19 -1.2 2,231,444 + DVU2135 0.19 +0.5 2,231,452 - DVU2135 0.20 +0.0 2,231,556 + DVU2135 0.39 -0.8 2,231,583 - DVU2135 0.44 -0.1 2,231,599 - DVU2135 0.47 -0.2 2,231,605 - DVU2135 0.48 -0.5 2,231,736 + DVU2135 0.72 -0.1 2,231,736 + DVU2135 0.72 -1.5 2,231,871 - +0.1 2,231,890 + +0.7 2,231,923 + -1.1 2,231,947 + -0.2 2,232,057 + +0.7 2,232,064 + -1.7 2,232,133 - DVU2136 0.14 -0.6 2,232,181 - DVU2136 0.18 +0.5 2,232,245 + DVU2136 0.23 -0.2 2,232,411 + DVU2136 0.37 -0.4 2,232,411 + DVU2136 0.37 -1.3 2,232,419 - DVU2136 0.38 -0.0 2,232,446 - DVU2136 0.40 -0.3 2,232,564 + DVU2136 0.50 -1.1 2,232,572 - DVU2136 0.51 -0.5 2,232,572 - DVU2136 0.51 +0.5 2,232,572 - DVU2136 0.51 -0.3 2,232,579 + DVU2136 0.52 -0.7 2,232,587 - DVU2136 0.52 -0.2 2,232,642 + DVU2136 0.57 -0.5 2,232,650 + DVU2136 0.58 -0.7 2,232,668 - DVU2136 0.59 -0.2 2,232,730 - DVU2136 0.64 -0.1 2,232,744 - DVU2136 0.66 -0.9 2,232,746 + DVU2136 0.66 -0.8 2,232,770 + DVU2136 0.68 -0.4 2,232,841 - DVU2136 0.74 +0.1 2,232,866 + DVU2136 0.76 -1.0 2,232,881 - DVU2136 0.77 -1.2 2,232,889 + DVU2136 0.78 -0.0 2,232,895 - DVU2136 0.78 -1.2 2,232,897 - DVU2136 0.79 +0.4 2,232,929 + DVU2136 0.81 -0.2 2,232,935 + DVU2136 0.82 -0.3 2,233,035 + -0.4 2,233,053 - -0.3 2,233,092 - -0.9 2,233,118 + +0.1 2,233,222 + +0.1 2,233,237 - +0.1 2,233,327 + +1.5 2,233,382 - -0.2 2,233,502 + -0.3 2,233,510 - +0.1 2,233,663 + sucCD DVU2137 0.14 -2.9 2,233,739 - sucCD DVU2137 0.17 +0.3 2,233,935 + sucCD DVU2137 0.26 -1.1 2,233,936 - sucCD DVU2137 0.27 +1.0 2,233,954 + sucCD DVU2137 0.27 +1.5 2,234,087 - sucCD DVU2137 0.34 -0.2 2,234,087 - sucCD DVU2137 0.34 +0.5 2,234,113 + sucCD DVU2137 0.35 -0.1 2,234,121 - sucCD DVU2137 0.35 -0.8
Or see this region's nucleotide sequence