Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1790 and DVU1791 are separated by 6 nucleotides DVU1791 and rpsU are separated by 115 nucleotides rpsU and DVU1794 are separated by 116 nucleotides DVU1794 and hup-3 are separated by 10 nucleotides hup-3 and DVU1796 overlap by 4 nucleotides
DVU1790: DVU1790 - MutS2 family protein (TIGR), at 1,854,518 to 1,856,833
DVU1790
DVU1791: DVU1791 - GatB/Yqey family protein (TIGR), at 1,856,840 to 1,857,289
DVU1791
DVU1792: rpsU - ribosomal protein S21 (TIGR), at 1,857,405 to 1,857,608
rpsU
DVU1794: DVU1794 - hypothetical protein (TIGR), at 1,857,725 to 1,857,928
DVU1794
DVU1795: hup-3 - DNA-binding protein HU (TIGR), at 1,857,939 to 1,858,211
hup-3
DVU1796: DVU1796 - hypothetical protein (TIGR), at 1,858,208 to 1,858,330
DVU1796
Position (kb)
1856
1857
1858 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1855.848 kb on - strand, within DVU1790 at 1855.865 kb on + strand, within DVU1790 at 1855.873 kb on - strand, within DVU1790 at 1855.939 kb on - strand, within DVU1790 at 1855.988 kb on - strand, within DVU1790 at 1855.993 kb on + strand, within DVU1790 at 1856.014 kb on + strand, within DVU1790 at 1856.014 kb on + strand, within DVU1790 at 1856.014 kb on + strand, within DVU1790 at 1856.162 kb on + strand, within DVU1790 at 1856.162 kb on + strand, within DVU1790 at 1856.170 kb on - strand, within DVU1790 at 1856.299 kb on - strand, within DVU1790 at 1856.306 kb on + strand, within DVU1790 at 1856.314 kb on - strand, within DVU1790 at 1856.314 kb on - strand, within DVU1790 at 1856.416 kb on + strand, within DVU1790 at 1856.417 kb on - strand, within DVU1790 at 1856.566 kb on + strand, within DVU1790 at 1856.628 kb on - strand at 1856.666 kb on - strand at 1856.681 kb on - strand at 1856.681 kb on - strand at 1856.691 kb on - strand at 1856.728 kb on + strand at 1856.773 kb on - strand at 1856.788 kb on - strand at 1856.849 kb on + strand at 1856.849 kb on + strand at 1856.978 kb on + strand, within DVU1791 at 1857.066 kb on + strand, within DVU1791 at 1857.084 kb on + strand, within DVU1791 at 1857.231 kb on - strand, within DVU1791 at 1857.237 kb on - strand, within DVU1791 at 1857.655 kb on + strand at 1857.663 kb on - strand at 1857.799 kb on + strand, within DVU1794 at 1857.824 kb on - strand, within DVU1794 at 1857.824 kb on - strand, within DVU1794 at 1857.824 kb on - strand, within DVU1794 at 1857.863 kb on - strand, within DVU1794 at 1858.109 kb on - strand, within hup-3 at 1858.248 kb on + strand, within DVU1796 at 1858.248 kb on + strand, within DVU1796
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 1,855,848 - DVU1790 0.57 +0.0 1,855,865 + DVU1790 0.58 -0.1 1,855,873 - DVU1790 0.59 +0.2 1,855,939 - DVU1790 0.61 +0.3 1,855,988 - DVU1790 0.63 +0.9 1,855,993 + DVU1790 0.64 -0.3 1,856,014 + DVU1790 0.65 +1.5 1,856,014 + DVU1790 0.65 -0.5 1,856,014 + DVU1790 0.65 +1.0 1,856,162 + DVU1790 0.71 +0.1 1,856,162 + DVU1790 0.71 -0.1 1,856,170 - DVU1790 0.71 -0.8 1,856,299 - DVU1790 0.77 -0.4 1,856,306 + DVU1790 0.77 -0.5 1,856,314 - DVU1790 0.78 -0.8 1,856,314 - DVU1790 0.78 -1.0 1,856,416 + DVU1790 0.82 -0.1 1,856,417 - DVU1790 0.82 -0.6 1,856,566 + DVU1790 0.88 +0.2 1,856,628 - -0.4 1,856,666 - -4.2 1,856,681 - -0.3 1,856,681 - +1.2 1,856,691 - +0.2 1,856,728 + -1.0 1,856,773 - +0.2 1,856,788 - -0.8 1,856,849 + -0.8 1,856,849 + -1.5 1,856,978 + DVU1791 0.31 -0.9 1,857,066 + DVU1791 0.50 -0.2 1,857,084 + DVU1791 0.54 +0.6 1,857,231 - DVU1791 0.87 +0.1 1,857,237 - DVU1791 0.88 -0.9 1,857,655 + -0.0 1,857,663 - -0.6 1,857,799 + DVU1794 0.36 +0.1 1,857,824 - DVU1794 0.49 -0.0 1,857,824 - DVU1794 0.49 +0.0 1,857,824 - DVU1794 0.49 -0.6 1,857,863 - DVU1794 0.68 -1.2 1,858,109 - hup-3 DVU1795 0.62 -1.3 1,858,248 + DVU1796 0.33 +0.6 1,858,248 + DVU1796 0.33 -0.1
Or see this region's nucleotide sequence