Experiment: MoYLS4 with Spectinomycin 0.025 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gpmA and DVU1620 are separated by 4 nucleotides DVU1620 and DVU1621 are separated by 101 nucleotides DVU1621 and DORF12360 are separated by 145 nucleotides DORF12360 and purQ are separated by 52 nucleotides
DVU1619: gpmA - phosphoglycerate mutase, 2,3-bisphosphoglycerate-independent (TIGR), at 1,703,251 to 1,704,786
gpmA
DVU1620: DVU1620 - hypothetical protein (TIGR), at 1,704,791 to 1,705,033
DVU1620
DVU1621: DVU1621 - hypothetical protein (TIGR), at 1,705,135 to 1,705,557
DVU1621
DORF12360: DORF12360 - SEED: FIG00605053: hypothetical protein, at 1,705,703 to 1,706,176
DORF12360
DVU1622: purQ - phosphoribosylformylglycinamidine synthase I (TIGR), at 1,706,229 to 1,707,038
purQ
Position (kb)
1705
1706
1707 Strain fitness (log2 ratio)
-2
-1
0
1 at 1704.914 kb on + strand, within DVU1620 at 1704.937 kb on + strand, within DVU1620 at 1705.077 kb on + strand at 1705.193 kb on - strand, within DVU1621 at 1705.337 kb on + strand, within DVU1621 at 1705.566 kb on + strand at 1705.592 kb on + strand at 1705.713 kb on + strand at 1705.720 kb on + strand at 1705.720 kb on + strand at 1705.745 kb on - strand at 1705.762 kb on - strand, within DORF12360 at 1705.824 kb on + strand, within DORF12360 at 1705.910 kb on - strand, within DORF12360 at 1705.944 kb on + strand, within DORF12360 at 1706.080 kb on - strand, within DORF12360 at 1707.103 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Spectinomycin 0.025 mg/mL remove 1,704,914 + DVU1620 0.51 -1.9 1,704,937 + DVU1620 0.60 -1.0 1,705,077 + -0.8 1,705,193 - DVU1621 0.14 -0.1 1,705,337 + DVU1621 0.48 -0.4 1,705,566 + +1.4 1,705,592 + -0.1 1,705,713 + -0.3 1,705,720 + +1.5 1,705,720 + -0.4 1,705,745 - -0.4 1,705,762 - DORF12360 0.12 -0.3 1,705,824 + DORF12360 0.26 -0.6 1,705,910 - DORF12360 0.44 +0.6 1,705,944 + DORF12360 0.51 -1.7 1,706,080 - DORF12360 0.80 -1.3 1,707,103 + -0.6
Or see this region's nucleotide sequence