Experiment: MoYLS4 with Chloride 62.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpF and glpK are separated by 146 nucleotides glpK and mdaB are separated by 262 nucleotides mdaB and DVU3136 are separated by 55 nucleotides
DVU3133: glpF - glycerol uptake facilitator protein (TIGR), at 3,280,115 to 3,280,870
glpF
DVU3134: glpK - glycerol kinase (TIGR), at 3,281,017 to 3,282,510
glpK
DVU3135: mdaB - flavodoxin-like fold domain protein (TIGR), at 3,282,773 to 3,283,381
mdaB
DVU3136: DVU3136 - nitroreductase family protein (TIGR), at 3,283,437 to 3,284,093
DVU3136
Position (kb)
3281
3282
3283 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3280.027 kb on + strand at 3280.046 kb on - strand at 3280.223 kb on + strand, within glpF at 3280.297 kb on + strand, within glpF at 3280.305 kb on - strand, within glpF at 3280.375 kb on + strand, within glpF at 3280.458 kb on - strand, within glpF at 3280.460 kb on + strand, within glpF at 3280.463 kb on - strand, within glpF at 3280.468 kb on - strand, within glpF at 3280.598 kb on + strand, within glpF at 3280.788 kb on - strand, within glpF at 3280.859 kb on + strand at 3280.859 kb on + strand at 3280.859 kb on + strand at 3280.867 kb on - strand at 3280.867 kb on - strand at 3280.867 kb on - strand at 3280.879 kb on - strand at 3280.919 kb on + strand at 3280.921 kb on + strand at 3280.973 kb on + strand at 3281.008 kb on - strand at 3281.015 kb on - strand at 3281.036 kb on + strand at 3281.066 kb on + strand at 3281.124 kb on + strand at 3281.134 kb on + strand at 3281.189 kb on - strand, within glpK at 3281.232 kb on + strand, within glpK at 3281.249 kb on + strand, within glpK at 3281.339 kb on - strand, within glpK at 3281.515 kb on + strand, within glpK at 3281.522 kb on + strand, within glpK at 3281.560 kb on - strand, within glpK at 3281.562 kb on + strand, within glpK at 3281.791 kb on + strand, within glpK at 3281.857 kb on - strand, within glpK at 3281.894 kb on + strand, within glpK at 3281.952 kb on - strand, within glpK at 3281.976 kb on + strand, within glpK at 3281.984 kb on - strand, within glpK at 3282.010 kb on - strand, within glpK at 3282.074 kb on + strand, within glpK at 3282.081 kb on - strand, within glpK at 3282.201 kb on + strand, within glpK at 3282.270 kb on + strand, within glpK at 3282.270 kb on + strand, within glpK at 3282.295 kb on + strand, within glpK at 3282.313 kb on - strand, within glpK at 3282.339 kb on + strand, within glpK at 3282.492 kb on + strand at 3282.543 kb on - strand at 3282.599 kb on + strand at 3282.599 kb on + strand at 3282.599 kb on + strand at 3282.609 kb on - strand at 3282.747 kb on + strand at 3282.755 kb on - strand at 3282.813 kb on + strand at 3282.865 kb on + strand, within mdaB at 3282.935 kb on - strand, within mdaB at 3282.943 kb on + strand, within mdaB at 3283.085 kb on + strand, within mdaB at 3283.105 kb on - strand, within mdaB at 3283.131 kb on - strand, within mdaB at 3283.176 kb on + strand, within mdaB at 3283.180 kb on - strand, within mdaB at 3283.181 kb on + strand, within mdaB at 3283.214 kb on + strand, within mdaB at 3283.269 kb on - strand, within mdaB at 3283.420 kb on + strand at 3283.463 kb on - strand at 3283.466 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoYLS4 with Chloride 62.5 mM remove 3,280,027 + -0.0 3,280,046 - -0.7 3,280,223 + glpF DVU3133 0.14 -1.5 3,280,297 + glpF DVU3133 0.24 -0.2 3,280,305 - glpF DVU3133 0.25 -0.2 3,280,375 + glpF DVU3133 0.34 +1.0 3,280,458 - glpF DVU3133 0.45 -0.8 3,280,460 + glpF DVU3133 0.46 -0.7 3,280,463 - glpF DVU3133 0.46 -0.9 3,280,468 - glpF DVU3133 0.47 +0.7 3,280,598 + glpF DVU3133 0.64 +1.1 3,280,788 - glpF DVU3133 0.89 -0.4 3,280,859 + +0.6 3,280,859 + +1.0 3,280,859 + -0.3 3,280,867 - -0.7 3,280,867 - +2.3 3,280,867 - -1.7 3,280,879 - +0.3 3,280,919 + -0.5 3,280,921 + -1.0 3,280,973 + -0.4 3,281,008 - +0.4 3,281,015 - +0.8 3,281,036 + -0.2 3,281,066 + -2.1 3,281,124 + -0.3 3,281,134 + -0.2 3,281,189 - glpK DVU3134 0.12 -0.4 3,281,232 + glpK DVU3134 0.14 +0.1 3,281,249 + glpK DVU3134 0.16 -0.2 3,281,339 - glpK DVU3134 0.22 -0.0 3,281,515 + glpK DVU3134 0.33 -0.1 3,281,522 + glpK DVU3134 0.34 +1.2 3,281,560 - glpK DVU3134 0.36 +0.4 3,281,562 + glpK DVU3134 0.36 -1.3 3,281,791 + glpK DVU3134 0.52 -0.2 3,281,857 - glpK DVU3134 0.56 -1.5 3,281,894 + glpK DVU3134 0.59 -1.2 3,281,952 - glpK DVU3134 0.63 -0.3 3,281,976 + glpK DVU3134 0.64 -1.0 3,281,984 - glpK DVU3134 0.65 -1.2 3,282,010 - glpK DVU3134 0.66 -0.8 3,282,074 + glpK DVU3134 0.71 +0.7 3,282,081 - glpK DVU3134 0.71 -0.2 3,282,201 + glpK DVU3134 0.79 -2.8 3,282,270 + glpK DVU3134 0.84 +2.3 3,282,270 + glpK DVU3134 0.84 -0.5 3,282,295 + glpK DVU3134 0.86 -2.0 3,282,313 - glpK DVU3134 0.87 -1.2 3,282,339 + glpK DVU3134 0.88 -0.6 3,282,492 + +0.7 3,282,543 - +0.3 3,282,599 + +0.1 3,282,599 + +0.0 3,282,599 + +0.2 3,282,609 - -0.1 3,282,747 + -0.1 3,282,755 - -0.5 3,282,813 + +0.4 3,282,865 + mdaB DVU3135 0.15 -1.1 3,282,935 - mdaB DVU3135 0.27 -0.3 3,282,943 + mdaB DVU3135 0.28 -0.2 3,283,085 + mdaB DVU3135 0.51 +0.1 3,283,105 - mdaB DVU3135 0.55 +1.5 3,283,131 - mdaB DVU3135 0.59 +0.0 3,283,176 + mdaB DVU3135 0.66 -0.5 3,283,180 - mdaB DVU3135 0.67 -0.2 3,283,181 + mdaB DVU3135 0.67 -1.8 3,283,214 + mdaB DVU3135 0.72 -0.1 3,283,269 - mdaB DVU3135 0.81 -0.1 3,283,420 + +0.1 3,283,463 - +0.2 3,283,466 - -0.3
Or see this region's nucleotide sequence