Experiment: Lactate-Sulfate (60-30 mM) minimal (MoLS4)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1402 and cobO are separated by 4 nucleotides cobO and pflA overlap by 23 nucleotides
DVU1402: DVU1402 - transcriptional regulator, LysR family (TIGR), at 1,471,525 to 1,472,520
DVU1402
DVU1403: cobO - cob(I)alamin adenosyltransferase (TIGR), at 1,472,525 to 1,473,028
cobO
DVU1404: pflA - radical SAM domain protein (TIGR), at 1,473,006 to 1,474,112
pflA
Position (kb)
1472
1473
1474 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1471.678 kb on - strand, within DVU1402 at 1471.680 kb on + strand, within DVU1402 at 1471.707 kb on - strand, within DVU1402 at 1471.745 kb on - strand, within DVU1402 at 1471.750 kb on - strand, within DVU1402 at 1471.759 kb on - strand, within DVU1402 at 1471.907 kb on + strand, within DVU1402 at 1471.910 kb on - strand, within DVU1402 at 1471.948 kb on - strand, within DVU1402 at 1472.056 kb on + strand, within DVU1402 at 1472.074 kb on - strand, within DVU1402 at 1472.111 kb on + strand, within DVU1402 at 1472.119 kb on - strand, within DVU1402 at 1472.163 kb on + strand, within DVU1402 at 1472.187 kb on - strand, within DVU1402 at 1472.233 kb on + strand, within DVU1402 at 1472.257 kb on + strand, within DVU1402 at 1472.257 kb on + strand, within DVU1402 at 1472.257 kb on + strand, within DVU1402 at 1472.309 kb on - strand, within DVU1402 at 1472.343 kb on + strand, within DVU1402 at 1472.664 kb on + strand, within cobO at 1472.767 kb on - strand, within cobO at 1472.787 kb on + strand, within cobO at 1472.830 kb on - strand, within cobO at 1472.830 kb on - strand, within cobO at 1472.835 kb on - strand, within cobO at 1472.913 kb on - strand, within cobO at 1472.969 kb on + strand, within cobO at 1472.974 kb on + strand, within cobO at 1472.976 kb on - strand, within cobO at 1472.977 kb on - strand, within cobO at 1472.990 kb on + strand at 1472.990 kb on + strand at 1472.998 kb on - strand at 1473.036 kb on - strand at 1473.047 kb on + strand at 1473.087 kb on - strand at 1473.099 kb on + strand at 1473.175 kb on - strand, within pflA at 1473.175 kb on - strand, within pflA at 1473.212 kb on + strand, within pflA at 1473.258 kb on - strand, within pflA at 1473.260 kb on - strand, within pflA at 1473.281 kb on + strand, within pflA at 1473.289 kb on - strand, within pflA at 1473.300 kb on - strand, within pflA at 1473.318 kb on + strand, within pflA at 1473.328 kb on - strand, within pflA at 1473.455 kb on + strand, within pflA at 1473.475 kb on - strand, within pflA at 1473.489 kb on + strand, within pflA at 1473.610 kb on - strand, within pflA at 1473.645 kb on + strand, within pflA at 1473.668 kb on + strand, within pflA at 1473.677 kb on - strand, within pflA at 1473.766 kb on - strand, within pflA at 1473.805 kb on + strand, within pflA at 1473.813 kb on - strand, within pflA at 1473.813 kb on - strand, within pflA at 1473.813 kb on - strand, within pflA at 1473.870 kb on + strand, within pflA at 1473.870 kb on + strand, within pflA at 1473.878 kb on - strand, within pflA at 1473.941 kb on - strand, within pflA at 1473.955 kb on + strand, within pflA at 1473.963 kb on - strand, within pflA
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Sulfate (60-30 mM) minimal (MoLS4) remove 1,471,678 - DVU1402 0.15 +0.3 1,471,680 + DVU1402 0.16 +0.3 1,471,707 - DVU1402 0.18 +0.3 1,471,745 - DVU1402 0.22 -0.4 1,471,750 - DVU1402 0.23 -0.4 1,471,759 - DVU1402 0.23 -0.2 1,471,907 + DVU1402 0.38 -0.5 1,471,910 - DVU1402 0.39 +0.4 1,471,948 - DVU1402 0.42 -0.4 1,472,056 + DVU1402 0.53 -1.0 1,472,074 - DVU1402 0.55 +0.8 1,472,111 + DVU1402 0.59 -0.2 1,472,119 - DVU1402 0.60 -0.5 1,472,163 + DVU1402 0.64 -0.4 1,472,187 - DVU1402 0.66 +0.5 1,472,233 + DVU1402 0.71 -0.3 1,472,257 + DVU1402 0.73 -1.6 1,472,257 + DVU1402 0.73 -0.3 1,472,257 + DVU1402 0.73 -0.7 1,472,309 - DVU1402 0.79 -0.3 1,472,343 + DVU1402 0.82 -0.3 1,472,664 + cobO DVU1403 0.28 -4.7 1,472,767 - cobO DVU1403 0.48 -1.5 1,472,787 + cobO DVU1403 0.52 -2.2 1,472,830 - cobO DVU1403 0.61 -2.7 1,472,830 - cobO DVU1403 0.61 -2.3 1,472,835 - cobO DVU1403 0.62 -3.0 1,472,913 - cobO DVU1403 0.77 -1.8 1,472,969 + cobO DVU1403 0.88 -2.2 1,472,974 + cobO DVU1403 0.89 -3.6 1,472,976 - cobO DVU1403 0.89 -4.1 1,472,977 - cobO DVU1403 0.90 -3.4 1,472,990 + -2.8 1,472,990 + -1.6 1,472,998 - -2.3 1,473,036 - -0.4 1,473,047 + +0.2 1,473,087 - -0.1 1,473,099 + -0.0 1,473,175 - pflA DVU1404 0.15 -0.1 1,473,175 - pflA DVU1404 0.15 +0.2 1,473,212 + pflA DVU1404 0.19 -0.3 1,473,258 - pflA DVU1404 0.23 -0.1 1,473,260 - pflA DVU1404 0.23 +0.1 1,473,281 + pflA DVU1404 0.25 -0.6 1,473,289 - pflA DVU1404 0.26 -0.6 1,473,300 - pflA DVU1404 0.27 +0.1 1,473,318 + pflA DVU1404 0.28 +0.4 1,473,328 - pflA DVU1404 0.29 -0.3 1,473,455 + pflA DVU1404 0.41 -0.3 1,473,475 - pflA DVU1404 0.42 +0.1 1,473,489 + pflA DVU1404 0.44 -0.5 1,473,610 - pflA DVU1404 0.55 +0.5 1,473,645 + pflA DVU1404 0.58 +0.1 1,473,668 + pflA DVU1404 0.60 -0.3 1,473,677 - pflA DVU1404 0.61 +0.2 1,473,766 - pflA DVU1404 0.69 -1.7 1,473,805 + pflA DVU1404 0.72 -0.3 1,473,813 - pflA DVU1404 0.73 -0.0 1,473,813 - pflA DVU1404 0.73 +0.0 1,473,813 - pflA DVU1404 0.73 -0.6 1,473,870 + pflA DVU1404 0.78 -1.4 1,473,870 + pflA DVU1404 0.78 +0.1 1,473,878 - pflA DVU1404 0.79 +0.6 1,473,941 - pflA DVU1404 0.84 -1.3 1,473,955 + pflA DVU1404 0.86 -0.0 1,473,963 - pflA DVU1404 0.86 +0.8
Or see this region's nucleotide sequence