Experiment: Lactate-Sulfate (60-30 mM) minimal (MoLS4)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1013 and rtxB are separated by 401 nucleotides rtxB and rtxD are separated by 2 nucleotides
DVU1013: DVU1013 - type I secretion outer membrane protein, TolC family (TIGR), at 1,115,282 to 1,116,652
DVU1013
DVU1017: rtxB - ABC transporter, ATP-binding protein/permease protein (TIGR), at 1,117,054 to 1,119,384
rtxB
DVU1018: rtxD - type I secretion membrane fusion protein, HlyD family (TIGR), at 1,119,387 to 1,120,733
rtxD
Position (kb)
1117
1118
1119
1120 Strain fitness (log2 ratio)
-1
0
1 at 1116.081 kb on + strand, within DVU1013 at 1116.083 kb on - strand, within DVU1013 at 1116.088 kb on - strand, within DVU1013 at 1116.092 kb on + strand, within DVU1013 at 1116.138 kb on + strand, within DVU1013 at 1116.148 kb on + strand, within DVU1013 at 1116.188 kb on - strand, within DVU1013 at 1116.199 kb on - strand, within DVU1013 at 1116.249 kb on + strand, within DVU1013 at 1116.271 kb on + strand, within DVU1013 at 1116.281 kb on + strand, within DVU1013 at 1116.309 kb on + strand, within DVU1013 at 1116.370 kb on + strand, within DVU1013 at 1116.389 kb on + strand, within DVU1013 at 1116.826 kb on + strand at 1116.944 kb on - strand at 1116.965 kb on - strand at 1116.965 kb on - strand at 1117.009 kb on - strand at 1117.045 kb on - strand at 1117.105 kb on + strand at 1117.117 kb on + strand at 1117.117 kb on + strand at 1117.125 kb on - strand at 1117.125 kb on - strand at 1117.127 kb on + strand at 1117.236 kb on + strand at 1117.236 kb on - strand at 1117.425 kb on + strand, within rtxB at 1117.438 kb on - strand, within rtxB at 1117.478 kb on + strand, within rtxB at 1117.481 kb on - strand, within rtxB at 1117.573 kb on + strand, within rtxB at 1117.634 kb on - strand, within rtxB at 1117.643 kb on + strand, within rtxB at 1117.659 kb on + strand, within rtxB at 1117.765 kb on + strand, within rtxB at 1117.819 kb on + strand, within rtxB at 1117.920 kb on + strand, within rtxB at 1117.920 kb on + strand, within rtxB at 1117.993 kb on - strand, within rtxB at 1118.144 kb on - strand, within rtxB at 1118.144 kb on - strand, within rtxB at 1118.144 kb on - strand, within rtxB at 1118.258 kb on - strand, within rtxB at 1118.401 kb on + strand, within rtxB at 1118.402 kb on - strand, within rtxB at 1118.402 kb on - strand, within rtxB at 1118.432 kb on + strand, within rtxB at 1118.475 kb on - strand, within rtxB at 1118.679 kb on - strand, within rtxB at 1118.787 kb on + strand, within rtxB at 1118.842 kb on - strand, within rtxB at 1119.001 kb on + strand, within rtxB at 1119.009 kb on - strand, within rtxB at 1119.090 kb on - strand, within rtxB at 1119.299 kb on - strand at 1119.303 kb on + strand at 1119.379 kb on + strand at 1119.490 kb on - strand at 1119.491 kb on - strand at 1119.581 kb on + strand, within rtxD at 1119.581 kb on + strand, within rtxD at 1119.590 kb on - strand, within rtxD at 1119.595 kb on - strand, within rtxD at 1119.601 kb on + strand, within rtxD at 1119.604 kb on + strand, within rtxD at 1119.611 kb on + strand, within rtxD at 1119.612 kb on - strand, within rtxD at 1119.712 kb on - strand, within rtxD at 1119.738 kb on - strand, within rtxD at 1120.058 kb on + strand, within rtxD at 1120.066 kb on - strand, within rtxD at 1120.150 kb on - strand, within rtxD at 1120.234 kb on - strand, within rtxD at 1120.242 kb on - strand, within rtxD at 1120.275 kb on - strand, within rtxD
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Sulfate (60-30 mM) minimal (MoLS4) remove 1,116,081 + DVU1013 0.58 +0.6 1,116,083 - DVU1013 0.58 +0.3 1,116,088 - DVU1013 0.59 -0.2 1,116,092 + DVU1013 0.59 -0.6 1,116,138 + DVU1013 0.62 -0.4 1,116,148 + DVU1013 0.63 +0.1 1,116,188 - DVU1013 0.66 -0.1 1,116,199 - DVU1013 0.67 +0.0 1,116,249 + DVU1013 0.71 +0.1 1,116,271 + DVU1013 0.72 -0.3 1,116,281 + DVU1013 0.73 +0.2 1,116,309 + DVU1013 0.75 +0.2 1,116,370 + DVU1013 0.79 +0.4 1,116,389 + DVU1013 0.81 +0.3 1,116,826 + +0.1 1,116,944 - +1.0 1,116,965 - -0.6 1,116,965 - -0.1 1,117,009 - -0.6 1,117,045 - -1.0 1,117,105 + -0.5 1,117,117 + +0.3 1,117,117 + +0.2 1,117,125 - +0.3 1,117,125 - +0.7 1,117,127 + -1.1 1,117,236 + -0.3 1,117,236 - -0.2 1,117,425 + rtxB DVU1017 0.16 -0.2 1,117,438 - rtxB DVU1017 0.16 -0.1 1,117,478 + rtxB DVU1017 0.18 +0.8 1,117,481 - rtxB DVU1017 0.18 +0.1 1,117,573 + rtxB DVU1017 0.22 +0.9 1,117,634 - rtxB DVU1017 0.25 +0.1 1,117,643 + rtxB DVU1017 0.25 -0.2 1,117,659 + rtxB DVU1017 0.26 -0.2 1,117,765 + rtxB DVU1017 0.31 -0.4 1,117,819 + rtxB DVU1017 0.33 +0.6 1,117,920 + rtxB DVU1017 0.37 -0.3 1,117,920 + rtxB DVU1017 0.37 +0.3 1,117,993 - rtxB DVU1017 0.40 +0.2 1,118,144 - rtxB DVU1017 0.47 -0.5 1,118,144 - rtxB DVU1017 0.47 +0.9 1,118,144 - rtxB DVU1017 0.47 +0.1 1,118,258 - rtxB DVU1017 0.52 -1.2 1,118,401 + rtxB DVU1017 0.58 +0.0 1,118,402 - rtxB DVU1017 0.58 +0.5 1,118,402 - rtxB DVU1017 0.58 +1.4 1,118,432 + rtxB DVU1017 0.59 -0.2 1,118,475 - rtxB DVU1017 0.61 -0.2 1,118,679 - rtxB DVU1017 0.70 +0.5 1,118,787 + rtxB DVU1017 0.74 +0.2 1,118,842 - rtxB DVU1017 0.77 -0.4 1,119,001 + rtxB DVU1017 0.84 -0.1 1,119,009 - rtxB DVU1017 0.84 -0.1 1,119,090 - rtxB DVU1017 0.87 -0.5 1,119,299 - -0.1 1,119,303 + -0.2 1,119,379 + +0.8 1,119,490 - +0.1 1,119,491 - +0.3 1,119,581 + rtxD DVU1018 0.14 +0.4 1,119,581 + rtxD DVU1018 0.14 -0.2 1,119,590 - rtxD DVU1018 0.15 +0.1 1,119,595 - rtxD DVU1018 0.15 -0.6 1,119,601 + rtxD DVU1018 0.16 +1.2 1,119,604 + rtxD DVU1018 0.16 +0.3 1,119,611 + rtxD DVU1018 0.17 +0.3 1,119,612 - rtxD DVU1018 0.17 -0.8 1,119,712 - rtxD DVU1018 0.24 -0.0 1,119,738 - rtxD DVU1018 0.26 +0.5 1,120,058 + rtxD DVU1018 0.50 +0.2 1,120,066 - rtxD DVU1018 0.50 +0.4 1,120,150 - rtxD DVU1018 0.57 +0.2 1,120,234 - rtxD DVU1018 0.63 +0.5 1,120,242 - rtxD DVU1018 0.63 +0.3 1,120,275 - rtxD DVU1018 0.66 +1.0
Or see this region's nucleotide sequence