Experiment: Lactate-Sulfate (60-30 mM) minimal (MoLS4)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt prfB and DVU1862 overlap by 20 nucleotides DVU1862 and DVU1863 overlap by 4 nucleotides DVU1863 and ihfB are separated by 74 nucleotides ihfB and DVU1865 overlap by 14 nucleotides DVU1865 and DVU1866 are separated by 51 nucleotides
DVU1861: prfB - peptide chain release factor 2, programmed frameshift (TIGR), at 1,928,351 to 1,929,467
prfB
DVU1862: DVU1862 - GGDEF domain protein (TIGR), at 1,929,448 to 1,930,443
DVU1862
DVU1863: DVU1863 - flagellar synthesis regulator FleN, putative (TIGR), at 1,930,440 to 1,931,258
DVU1863
DVU1864: ihfB - DNA-binding protein HU, beta subunit, putative (TIGR), at 1,931,333 to 1,931,614
ihfB
DVU1865: DVU1865 - hypothetical protein (TIGR), at 1,931,601 to 1,931,741
DVU1865
DVU1866: DVU1866 - hypothetical protein (TIGR), at 1,931,793 to 1,932,494
DVU1866
Position (kb)
1930
1931
1932 Strain fitness (log2 ratio)
-1
0
1
2 at 1929.486 kb on - strand at 1929.486 kb on - strand at 1929.619 kb on + strand, within DVU1862 at 1929.649 kb on - strand, within DVU1862 at 1929.649 kb on - strand, within DVU1862 at 1929.685 kb on + strand, within DVU1862 at 1929.798 kb on + strand, within DVU1862 at 1929.943 kb on + strand, within DVU1862 at 1930.055 kb on + strand, within DVU1862 at 1930.101 kb on + strand, within DVU1862 at 1930.111 kb on + strand, within DVU1862 at 1930.138 kb on + strand, within DVU1862 at 1930.379 kb on - strand at 1930.387 kb on - strand at 1930.421 kb on + strand at 1930.421 kb on + strand at 1930.429 kb on - strand at 1930.467 kb on + strand at 1930.469 kb on - strand at 1930.499 kb on + strand at 1930.668 kb on - strand, within DVU1863 at 1930.673 kb on - strand, within DVU1863 at 1930.763 kb on - strand, within DVU1863 at 1930.779 kb on + strand, within DVU1863 at 1930.909 kb on + strand, within DVU1863 at 1930.927 kb on - strand, within DVU1863 at 1931.078 kb on + strand, within DVU1863 at 1931.090 kb on + strand, within DVU1863 at 1931.204 kb on - strand at 1931.252 kb on + strand at 1931.329 kb on - strand at 1931.350 kb on + strand at 1931.608 kb on + strand at 1931.729 kb on - strand at 1931.773 kb on + strand at 1931.864 kb on + strand, within DVU1866 at 1932.025 kb on + strand, within DVU1866 at 1932.151 kb on - strand, within DVU1866 at 1932.161 kb on - strand, within DVU1866 at 1932.174 kb on + strand, within DVU1866 at 1932.246 kb on + strand, within DVU1866
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Sulfate (60-30 mM) minimal (MoLS4) remove 1,929,486 - +0.0 1,929,486 - +0.8 1,929,619 + DVU1862 0.17 +1.8 1,929,649 - DVU1862 0.20 -1.5 1,929,649 - DVU1862 0.20 +0.2 1,929,685 + DVU1862 0.24 +0.5 1,929,798 + DVU1862 0.35 -0.3 1,929,943 + DVU1862 0.50 -0.6 1,930,055 + DVU1862 0.61 -0.2 1,930,101 + DVU1862 0.66 -0.7 1,930,111 + DVU1862 0.67 +0.1 1,930,138 + DVU1862 0.69 +0.3 1,930,379 - +1.7 1,930,387 - -0.2 1,930,421 + -0.7 1,930,421 + -0.3 1,930,429 - -0.2 1,930,467 + +0.4 1,930,469 - -0.3 1,930,499 + +0.0 1,930,668 - DVU1863 0.28 -0.2 1,930,673 - DVU1863 0.28 -0.0 1,930,763 - DVU1863 0.39 -0.2 1,930,779 + DVU1863 0.41 -0.2 1,930,909 + DVU1863 0.57 -0.2 1,930,927 - DVU1863 0.59 -0.3 1,931,078 + DVU1863 0.78 -0.5 1,931,090 + DVU1863 0.79 -0.1 1,931,204 - -0.7 1,931,252 + +0.2 1,931,329 - -1.3 1,931,350 + +1.0 1,931,608 + -0.7 1,931,729 - +0.1 1,931,773 + -0.2 1,931,864 + DVU1866 0.10 -0.0 1,932,025 + DVU1866 0.33 +0.0 1,932,151 - DVU1866 0.51 -0.3 1,932,161 - DVU1866 0.52 -0.5 1,932,174 + DVU1866 0.54 +0.2 1,932,246 + DVU1866 0.65 +0.2
Or see this region's nucleotide sequence