Experiment: Lactate-Sulfate (60-30 mM) minimal (MoLS4)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnB-2 and glnB-3 are separated by 9 nucleotides glnB-3 and nifH are separated by 282 nucleotides nifH and nifV are separated by 757 nucleotides
DVUA0013: glnB-2 - nitrogen regulatory protein P-II (TIGR), at 17,863 to 18,258
glnB-2
DVUA0014: glnB-3 - nitrogen regulatory protein P-II (TIGR), at 18,268 to 18,594
glnB-3
DVUA0015: nifH - nitrogenase iron protein (TIGR), at 18,877 to 19,710
nifH
DVUA0016: nifV - homocitrate synthase (TIGR), at 20,468 to 21,622
nifV
Position (kb) 18
19
20 Strain fitness (log2 ratio) -2
-1
0
1 at 17.919 kb on - strand, within glnB-2 at 17.997 kb on + strand, within glnB-2 at 18.002 kb on + strand, within glnB-2 at 18.061 kb on - strand, within glnB-2 at 18.085 kb on - strand, within glnB-2 at 18.186 kb on + strand, within glnB-2 at 18.242 kb on - strand at 18.282 kb on + strand at 18.296 kb on + strand at 18.296 kb on + strand at 18.296 kb on + strand at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.304 kb on - strand, within glnB-3 at 18.353 kb on + strand, within glnB-3 at 18.370 kb on + strand, within glnB-3 at 18.378 kb on - strand, within glnB-3 at 18.404 kb on + strand, within glnB-3 at 18.404 kb on + strand, within glnB-3 at 18.431 kb on + strand, within glnB-3 at 18.439 kb on - strand, within glnB-3 at 18.439 kb on - strand, within glnB-3 at 18.466 kb on - strand, within glnB-3 at 18.473 kb on - strand, within glnB-3 at 18.492 kb on - strand, within glnB-3 at 18.506 kb on - strand, within glnB-3 at 18.527 kb on + strand, within glnB-3 at 18.535 kb on - strand, within glnB-3 at 18.559 kb on - strand, within glnB-3 at 18.747 kb on + strand at 18.755 kb on - strand at 18.849 kb on - strand at 18.884 kb on + strand at 18.922 kb on - strand at 18.994 kb on - strand, within nifH at 19.054 kb on + strand, within nifH at 19.273 kb on - strand, within nifH at 19.273 kb on - strand, within nifH at 19.322 kb on + strand, within nifH at 19.405 kb on + strand, within nifH at 19.413 kb on - strand, within nifH at 19.429 kb on + strand, within nifH at 19.429 kb on + strand, within nifH at 19.440 kb on + strand, within nifH at 19.448 kb on - strand, within nifH at 19.448 kb on - strand, within nifH at 19.540 kb on - strand, within nifH at 19.600 kb on + strand, within nifH at 19.600 kb on + strand, within nifH at 19.641 kb on + strand at 19.652 kb on - strand at 19.732 kb on - strand at 19.780 kb on + strand at 19.801 kb on - strand at 19.838 kb on + strand at 19.927 kb on - strand at 19.938 kb on - strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.942 kb on + strand at 19.950 kb on - strand at 19.950 kb on - strand at 19.950 kb on - strand at 19.972 kb on - strand at 19.979 kb on + strand at 19.987 kb on - strand at 20.024 kb on + strand at 20.032 kb on - strand at 20.089 kb on - strand at 20.132 kb on - strand at 20.280 kb on - strand at 20.373 kb on + strand at 20.401 kb on + strand at 20.406 kb on + strand at 20.409 kb on - strand at 20.409 kb on - strand at 20.411 kb on + strand at 20.461 kb on + strand at 20.519 kb on + strand at 20.544 kb on + strand at 20.569 kb on + strand at 20.571 kb on - strand at 20.577 kb on - strand at 20.577 kb on - strand at 20.671 kb on - strand, within nifV at 20.699 kb on - strand, within nifV
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Sulfate (60-30 mM) minimal (MoLS4) remove 17,919 - glnB-2 DVUA0013 0.14 +0.7 17,997 + glnB-2 DVUA0013 0.34 -0.3 18,002 + glnB-2 DVUA0013 0.35 -0.5 18,061 - glnB-2 DVUA0013 0.50 -0.1 18,085 - glnB-2 DVUA0013 0.56 -0.1 18,186 + glnB-2 DVUA0013 0.82 +0.4 18,242 - -0.5 18,282 + -0.1 18,296 + +1.1 18,296 + +0.6 18,296 + -0.9 18,304 - glnB-3 DVUA0014 0.11 +0.1 18,304 - glnB-3 DVUA0014 0.11 +0.5 18,304 - glnB-3 DVUA0014 0.11 +0.7 18,304 - glnB-3 DVUA0014 0.11 -1.1 18,353 + glnB-3 DVUA0014 0.26 -0.4 18,370 + glnB-3 DVUA0014 0.31 +0.0 18,378 - glnB-3 DVUA0014 0.34 -0.4 18,404 + glnB-3 DVUA0014 0.42 -0.3 18,404 + glnB-3 DVUA0014 0.42 -0.3 18,431 + glnB-3 DVUA0014 0.50 -0.3 18,439 - glnB-3 DVUA0014 0.52 -0.2 18,439 - glnB-3 DVUA0014 0.52 -0.0 18,466 - glnB-3 DVUA0014 0.61 +0.1 18,473 - glnB-3 DVUA0014 0.63 +0.1 18,492 - glnB-3 DVUA0014 0.69 -0.1 18,506 - glnB-3 DVUA0014 0.73 -0.3 18,527 + glnB-3 DVUA0014 0.79 -0.2 18,535 - glnB-3 DVUA0014 0.82 -0.2 18,559 - glnB-3 DVUA0014 0.89 -0.2 18,747 + -0.8 18,755 - -0.8 18,849 - +0.5 18,884 + +0.0 18,922 - -0.1 18,994 - nifH DVUA0015 0.14 +0.2 19,054 + nifH DVUA0015 0.21 -0.1 19,273 - nifH DVUA0015 0.47 -0.5 19,273 - nifH DVUA0015 0.47 -1.0 19,322 + nifH DVUA0015 0.53 -2.1 19,405 + nifH DVUA0015 0.63 -0.8 19,413 - nifH DVUA0015 0.64 +0.1 19,429 + nifH DVUA0015 0.66 -0.6 19,429 + nifH DVUA0015 0.66 +0.3 19,440 + nifH DVUA0015 0.68 -0.5 19,448 - nifH DVUA0015 0.68 +0.1 19,448 - nifH DVUA0015 0.68 +0.4 19,540 - nifH DVUA0015 0.79 -0.8 19,600 + nifH DVUA0015 0.87 -0.6 19,600 + nifH DVUA0015 0.87 -0.7 19,641 + -0.2 19,652 - +0.1 19,732 - +0.3 19,780 + -0.2 19,801 - -0.9 19,838 + -0.1 19,927 - +0.3 19,938 - +0.0 19,942 + -0.4 19,942 + +1.4 19,942 + -0.3 19,942 + -0.0 19,942 + +0.1 19,942 + -1.1 19,942 + -0.3 19,950 - +0.5 19,950 - -0.1 19,950 - +0.3 19,972 - +0.7 19,979 + +0.1 19,987 - -0.5 20,024 + -1.0 20,032 - +1.4 20,089 - -0.5 20,132 - -0.8 20,280 - -0.1 20,373 + +0.4 20,401 + -0.4 20,406 + -0.6 20,409 - +0.8 20,409 - -0.1 20,411 + -0.4 20,461 + -0.4 20,519 + +0.3 20,544 + +0.2 20,569 + +0.7 20,571 - +0.2 20,577 - -0.0 20,577 - +0.5 20,671 - nifV DVUA0016 0.18 -0.6 20,699 - nifV DVUA0016 0.20 -0.4
Or see this region's nucleotide sequence