Experiment: Lactate-Sulfate (60-30 mM) minimal (MoLS4)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt accC and DVU2227 overlap by 20 nucleotides DVU2227 and DVU2228 are separated by 4 nucleotides DVU2228 and motA-2 overlap by 17 nucleotides
DVU2226: accC - acetyl-CoA carboxylase, biotin carboxylase, putative (TIGR), at 2,318,897 to 2,320,312
accC
DVU2227: DVU2227 - hypothetical protein (TIGR), at 2,320,293 to 2,320,736
DVU2227
DVU2228: DVU2228 - motB protein, putative (TIGR), at 2,320,741 to 2,321,475
DVU2228
DVU2229: motA-2 - chemotaxis protein MotA (TIGR), at 2,321,459 to 2,322,220
motA-2
Position (kb)
2320
2321 Strain fitness (log2 ratio)
-1
0
1 at 2320.431 kb on + strand, within DVU2227 at 2320.439 kb on - strand, within DVU2227 at 2320.534 kb on + strand, within DVU2227 at 2320.537 kb on - strand, within DVU2227 at 2320.570 kb on + strand, within DVU2227 at 2320.588 kb on - strand, within DVU2227 at 2320.663 kb on + strand, within DVU2227 at 2320.663 kb on + strand, within DVU2227 at 2320.673 kb on + strand, within DVU2227 at 2320.673 kb on + strand, within DVU2227 at 2320.673 kb on + strand, within DVU2227 at 2320.673 kb on + strand, within DVU2227 at 2320.689 kb on - strand, within DVU2227 at 2320.769 kb on + strand at 2320.769 kb on + strand at 2320.800 kb on + strand at 2320.939 kb on - strand, within DVU2228 at 2320.954 kb on + strand, within DVU2228 at 2321.044 kb on - strand, within DVU2228 at 2321.058 kb on + strand, within DVU2228 at 2321.093 kb on - strand, within DVU2228 at 2321.120 kb on - strand, within DVU2228 at 2321.152 kb on + strand, within DVU2228 at 2321.228 kb on + strand, within DVU2228 at 2321.236 kb on - strand, within DVU2228 at 2321.236 kb on - strand, within DVU2228 at 2321.529 kb on + strand at 2321.576 kb on - strand, within motA-2 at 2321.587 kb on - strand, within motA-2 at 2321.604 kb on + strand, within motA-2 at 2321.612 kb on - strand, within motA-2 at 2321.613 kb on - strand, within motA-2 at 2321.663 kb on - strand, within motA-2 at 2321.681 kb on + strand, within motA-2 at 2321.692 kb on + strand, within motA-2
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Sulfate (60-30 mM) minimal (MoLS4) remove 2,320,431 + DVU2227 0.31 -0.3 2,320,439 - DVU2227 0.33 -0.5 2,320,534 + DVU2227 0.54 -0.9 2,320,537 - DVU2227 0.55 -0.3 2,320,570 + DVU2227 0.62 +0.3 2,320,588 - DVU2227 0.66 -1.2 2,320,663 + DVU2227 0.83 -0.5 2,320,663 + DVU2227 0.83 -0.1 2,320,673 + DVU2227 0.86 -0.5 2,320,673 + DVU2227 0.86 -0.1 2,320,673 + DVU2227 0.86 -0.6 2,320,673 + DVU2227 0.86 -0.8 2,320,689 - DVU2227 0.89 -1.7 2,320,769 + +0.1 2,320,769 + -0.4 2,320,800 + -0.6 2,320,939 - DVU2228 0.27 -0.6 2,320,954 + DVU2228 0.29 +0.2 2,321,044 - DVU2228 0.41 +0.3 2,321,058 + DVU2228 0.43 -0.8 2,321,093 - DVU2228 0.48 +0.3 2,321,120 - DVU2228 0.52 +0.1 2,321,152 + DVU2228 0.56 +0.1 2,321,228 + DVU2228 0.66 +0.7 2,321,236 - DVU2228 0.67 -0.6 2,321,236 - DVU2228 0.67 +0.5 2,321,529 + -0.4 2,321,576 - motA-2 DVU2229 0.15 +0.1 2,321,587 - motA-2 DVU2229 0.17 +0.2 2,321,604 + motA-2 DVU2229 0.19 -0.5 2,321,612 - motA-2 DVU2229 0.20 +0.2 2,321,613 - motA-2 DVU2229 0.20 -1.7 2,321,663 - motA-2 DVU2229 0.27 -0.3 2,321,681 + motA-2 DVU2229 0.29 +0.1 2,321,692 + motA-2 DVU2229 0.31 +0.1
Or see this region's nucleotide sequence