Experiment: Lactate-Sulfate (60-30 mM) minimal (MoLS4)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt argG and argF are separated by 51 nucleotides argF and DVU1097 are separated by 141 nucleotides DVU1097 and tRNA-Thr are separated by 72 nucleotides
DVU1095: argG - argininosuccinate synthase (TIGR), at 1,200,783 to 1,201,973
argG
DVU1096: argF - ornithine carbamoyltransferase (TIGR), at 1,202,025 to 1,202,927
argF
DVU1097: DVU1097 - conserved hypothetical protein (TIGR), at 1,203,069 to 1,203,818
DVU1097
DVU_tRNA-Thr-2: tRNA-Thr - tRNA with anticodon CGT for Thr (VIMSS-AUTO), at 1,203,891 to 1,203,966
tRNA-Thr
Position (kb)
1202
1203 Strain fitness (log2 ratio)
-7
-6
-5
-4
-3
-2
-1
0
1 at 1201.069 kb on - strand, within argG at 1201.152 kb on + strand, within argG at 1201.251 kb on - strand, within argG at 1201.269 kb on - strand, within argG at 1201.291 kb on + strand, within argG at 1201.325 kb on - strand, within argG at 1201.325 kb on - strand, within argG at 1201.339 kb on - strand, within argG at 1201.371 kb on - strand, within argG at 1201.432 kb on + strand, within argG at 1201.452 kb on - strand, within argG at 1201.479 kb on + strand, within argG at 1201.498 kb on + strand, within argG at 1201.542 kb on - strand, within argG at 1201.562 kb on + strand, within argG at 1201.583 kb on + strand, within argG at 1201.593 kb on + strand, within argG at 1201.688 kb on - strand, within argG at 1201.691 kb on + strand, within argG at 1201.812 kb on - strand, within argG at 1201.819 kb on + strand, within argG at 1201.842 kb on - strand, within argG at 1201.859 kb on + strand at 1201.872 kb on + strand at 1201.894 kb on - strand at 1201.916 kb on - strand at 1201.947 kb on - strand at 1202.099 kb on + strand at 1202.147 kb on + strand, within argF at 1202.398 kb on + strand, within argF at 1202.398 kb on + strand, within argF at 1202.398 kb on + strand, within argF at 1202.406 kb on - strand, within argF at 1202.406 kb on - strand, within argF at 1202.406 kb on - strand, within argF at 1202.406 kb on - strand, within argF at 1202.406 kb on - strand, within argF at 1202.406 kb on - strand, within argF at 1202.487 kb on - strand, within argF at 1202.495 kb on + strand, within argF at 1202.520 kb on - strand, within argF at 1202.552 kb on + strand, within argF at 1202.618 kb on + strand, within argF at 1202.626 kb on - strand, within argF at 1202.643 kb on - strand, within argF at 1202.650 kb on - strand, within argF at 1202.650 kb on - strand, within argF at 1202.655 kb on - strand, within argF at 1202.683 kb on + strand, within argF at 1202.743 kb on - strand, within argF at 1202.765 kb on + strand, within argF at 1202.862 kb on - strand at 1202.981 kb on + strand at 1203.070 kb on + strand at 1203.097 kb on - strand at 1203.242 kb on + strand, within DVU1097 at 1203.250 kb on - strand, within DVU1097 at 1203.268 kb on - strand, within DVU1097 at 1203.283 kb on + strand, within DVU1097 at 1203.283 kb on + strand, within DVU1097 at 1203.302 kb on + strand, within DVU1097 at 1203.302 kb on + strand, within DVU1097 at 1203.310 kb on - strand, within DVU1097 at 1203.388 kb on - strand, within DVU1097 at 1203.474 kb on + strand, within DVU1097 at 1203.492 kb on - strand, within DVU1097 at 1203.547 kb on + strand, within DVU1097 at 1203.566 kb on - strand, within DVU1097 at 1203.731 kb on + strand, within DVU1097 at 1203.775 kb on - strand at 1203.804 kb on - strand at 1203.812 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lactate-Sulfate (60-30 mM) minimal (MoLS4) remove 1,201,069 - argG DVU1095 0.24 -5.6 1,201,152 + argG DVU1095 0.31 -5.6 1,201,251 - argG DVU1095 0.39 -2.7 1,201,269 - argG DVU1095 0.41 -1.1 1,201,291 + argG DVU1095 0.43 -5.7 1,201,325 - argG DVU1095 0.46 -3.8 1,201,325 - argG DVU1095 0.46 -3.3 1,201,339 - argG DVU1095 0.47 -4.3 1,201,371 - argG DVU1095 0.49 -2.7 1,201,432 + argG DVU1095 0.54 -4.2 1,201,452 - argG DVU1095 0.56 -4.7 1,201,479 + argG DVU1095 0.58 -2.7 1,201,498 + argG DVU1095 0.60 -0.4 1,201,542 - argG DVU1095 0.64 -0.6 1,201,562 + argG DVU1095 0.65 -1.6 1,201,583 + argG DVU1095 0.67 -5.9 1,201,593 + argG DVU1095 0.68 -4.3 1,201,688 - argG DVU1095 0.76 -4.7 1,201,691 + argG DVU1095 0.76 -7.0 1,201,812 - argG DVU1095 0.86 -4.3 1,201,819 + argG DVU1095 0.87 -4.4 1,201,842 - argG DVU1095 0.89 -3.8 1,201,859 + -3.1 1,201,872 + -5.0 1,201,894 - -3.2 1,201,916 - -2.4 1,201,947 - -4.0 1,202,099 + -4.1 1,202,147 + argF DVU1096 0.14 -1.7 1,202,398 + argF DVU1096 0.41 -3.4 1,202,398 + argF DVU1096 0.41 -5.2 1,202,398 + argF DVU1096 0.41 -3.5 1,202,406 - argF DVU1096 0.42 -2.7 1,202,406 - argF DVU1096 0.42 -3.9 1,202,406 - argF DVU1096 0.42 -5.3 1,202,406 - argF DVU1096 0.42 -2.5 1,202,406 - argF DVU1096 0.42 -3.4 1,202,406 - argF DVU1096 0.42 -6.3 1,202,487 - argF DVU1096 0.51 -6.6 1,202,495 + argF DVU1096 0.52 -4.4 1,202,520 - argF DVU1096 0.55 -0.9 1,202,552 + argF DVU1096 0.58 -5.7 1,202,618 + argF DVU1096 0.66 -0.9 1,202,626 - argF DVU1096 0.67 -2.6 1,202,643 - argF DVU1096 0.68 -0.9 1,202,650 - argF DVU1096 0.69 -5.4 1,202,650 - argF DVU1096 0.69 -4.6 1,202,655 - argF DVU1096 0.70 -3.1 1,202,683 + argF DVU1096 0.73 -4.4 1,202,743 - argF DVU1096 0.80 -0.6 1,202,765 + argF DVU1096 0.82 -2.1 1,202,862 - -5.0 1,202,981 + -2.1 1,203,070 + -1.0 1,203,097 - -4.2 1,203,242 + DVU1097 0.23 -0.9 1,203,250 - DVU1097 0.24 -2.3 1,203,268 - DVU1097 0.27 -4.8 1,203,283 + DVU1097 0.29 -3.7 1,203,283 + DVU1097 0.29 -4.8 1,203,302 + DVU1097 0.31 -2.2 1,203,302 + DVU1097 0.31 -2.2 1,203,310 - DVU1097 0.32 -3.7 1,203,388 - DVU1097 0.43 -3.1 1,203,474 + DVU1097 0.54 -2.8 1,203,492 - DVU1097 0.56 -3.1 1,203,547 + DVU1097 0.64 -3.6 1,203,566 - DVU1097 0.66 -4.6 1,203,731 + DVU1097 0.88 -2.6 1,203,775 - -1.4 1,203,804 - -1.1 1,203,812 + -0.2
Or see this region's nucleotide sequence