Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS23210

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS23200 and ECOLIN_RS23205 are separated by 65 nucleotidesECOLIN_RS23205 and ECOLIN_RS23210 overlap by 1 nucleotidesECOLIN_RS23210 and ECOLIN_RS23215 are separated by 9 nucleotidesECOLIN_RS23215 and ECOLIN_RS23220 are separated by 69 nucleotides ECOLIN_RS23200: ECOLIN_RS23200 - PTS mannose/fructose/sorbose transporter subunit IIC, at 4,669,390 to 4,670,187 _RS23200 ECOLIN_RS23205: ECOLIN_RS23205 - PTS system mannose/fructose/N-acetylgalactosamine-transporter subunit IIB, at 4,670,253 to 4,670,747 _RS23205 ECOLIN_RS23210: ECOLIN_RS23210 - mannose/fructose/sorbose PTS transporter subunit IIA, at 4,670,747 to 4,671,154 _RS23210 ECOLIN_RS23215: ECOLIN_RS23215 - SDR family oxidoreductase, at 4,671,164 to 4,671,970 _RS23215 ECOLIN_RS23220: ECOLIN_RS23220 - sugar-binding transcriptional regulator, at 4,672,040 to 4,672,987 _RS23220 Position (kb) 4670 4671 4672Strain fitness (log2 ratio) -2 -1 0 1at 4669.952 kb on - strand, within ECOLIN_RS23200at 4670.050 kb on + strand, within ECOLIN_RS23200at 4670.158 kb on - strandat 4670.232 kb on + strandat 4670.232 kb on + strandat 4670.350 kb on - strand, within ECOLIN_RS23205at 4670.370 kb on - strand, within ECOLIN_RS23205at 4670.507 kb on - strand, within ECOLIN_RS23205at 4670.636 kb on - strand, within ECOLIN_RS23205at 4670.739 kb on - strandat 4670.836 kb on + strand, within ECOLIN_RS23210at 4670.837 kb on - strand, within ECOLIN_RS23210at 4670.961 kb on - strand, within ECOLIN_RS23210at 4671.085 kb on - strand, within ECOLIN_RS23210at 4671.119 kb on + strandat 4671.193 kb on + strandat 4671.194 kb on - strandat 4671.213 kb on + strandat 4671.213 kb on + strandat 4671.214 kb on - strandat 4671.303 kb on + strand, within ECOLIN_RS23215at 4671.361 kb on - strand, within ECOLIN_RS23215at 4672.052 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,669,952 - ECOLIN_RS23200 0.70 -0.0
4,670,050 + ECOLIN_RS23200 0.83 +0.1
4,670,158 - +0.4
4,670,232 + -1.0
4,670,232 + +1.1
4,670,350 - ECOLIN_RS23205 0.20 -0.2
4,670,370 - ECOLIN_RS23205 0.24 +0.4
4,670,507 - ECOLIN_RS23205 0.51 -0.2
4,670,636 - ECOLIN_RS23205 0.77 +0.8
4,670,739 - -2.1
4,670,836 + ECOLIN_RS23210 0.22 +0.6
4,670,837 - ECOLIN_RS23210 0.22 +0.0
4,670,961 - ECOLIN_RS23210 0.52 +0.1
4,671,085 - ECOLIN_RS23210 0.83 +0.0
4,671,119 + -0.1
4,671,193 + -0.5
4,671,194 - +0.3
4,671,213 + -0.3
4,671,213 + -0.4
4,671,214 - +0.1
4,671,303 + ECOLIN_RS23215 0.17 -0.4
4,671,361 - ECOLIN_RS23215 0.24 +0.2
4,672,052 + -0.5

Or see this region's nucleotide sequence