Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS22140

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS22125 and ECOLIN_RS22130 are separated by 78 nucleotidesECOLIN_RS22130 and ECOLIN_RS22135 are separated by 3 nucleotidesECOLIN_RS22135 and ECOLIN_RS22140 are separated by 2 nucleotidesECOLIN_RS22140 and ECOLIN_RS22145 are separated by 41 nucleotidesECOLIN_RS22145 and ECOLIN_RS22150 overlap by 4 nucleotides ECOLIN_RS22125: ECOLIN_RS22125 - ubiquinone biosynthesis regulatory protein kinase UbiB, at 4,444,044 to 4,445,684 _RS22125 ECOLIN_RS22130: ECOLIN_RS22130 - Sec-independent protein translocase subunit TatA, at 4,445,763 to 4,446,032 _RS22130 ECOLIN_RS22135: ECOLIN_RS22135 - Sec-independent protein translocase protein TatB, at 4,446,036 to 4,446,551 _RS22135 ECOLIN_RS22140: ECOLIN_RS22140 - Sec-independent protein translocase subunit TatC, at 4,446,554 to 4,447,330 _RS22140 ECOLIN_RS22145: ECOLIN_RS22145 - 3'-5' ssDNA/RNA exonuclease TatD, at 4,447,372 to 4,448,154 _RS22145 ECOLIN_RS22150: ECOLIN_RS22150 - transcription/translation regulatory transformer protein RfaH, at 4,448,151 to 4,448,639 _RS22150 Position (kb) 4446 4447 4448Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1at 4445.712 kb on + strandat 4445.712 kb on + strandat 4445.717 kb on + strandat 4445.717 kb on + strandat 4445.717 kb on + strandat 4445.718 kb on - strandat 4445.718 kb on - strandat 4445.718 kb on - strandat 4445.718 kb on - strandat 4445.718 kb on - strandat 4445.762 kb on + strandat 4445.762 kb on + strandat 4445.763 kb on - strandat 4445.789 kb on - strandat 4445.812 kb on + strand, within ECOLIN_RS22130at 4445.812 kb on + strand, within ECOLIN_RS22130at 4445.812 kb on + strand, within ECOLIN_RS22130at 4446.289 kb on + strand, within ECOLIN_RS22135at 4446.321 kb on + strand, within ECOLIN_RS22135at 4446.321 kb on + strand, within ECOLIN_RS22135at 4446.321 kb on + strand, within ECOLIN_RS22135at 4446.322 kb on - strand, within ECOLIN_RS22135at 4446.542 kb on + strandat 4446.561 kb on + strandat 4446.561 kb on + strandat 4446.562 kb on - strandat 4446.659 kb on + strand, within ECOLIN_RS22140at 4446.678 kb on - strand, within ECOLIN_RS22140at 4446.678 kb on - strand, within ECOLIN_RS22140at 4446.781 kb on + strand, within ECOLIN_RS22140at 4446.781 kb on + strand, within ECOLIN_RS22140at 4446.851 kb on + strand, within ECOLIN_RS22140at 4446.851 kb on + strand, within ECOLIN_RS22140at 4446.852 kb on - strand, within ECOLIN_RS22140at 4446.853 kb on + strand, within ECOLIN_RS22140at 4446.908 kb on + strand, within ECOLIN_RS22140at 4447.017 kb on + strand, within ECOLIN_RS22140at 4447.017 kb on + strand, within ECOLIN_RS22140at 4447.017 kb on + strand, within ECOLIN_RS22140at 4447.017 kb on + strand, within ECOLIN_RS22140at 4447.017 kb on + strand, within ECOLIN_RS22140at 4447.018 kb on - strand, within ECOLIN_RS22140at 4447.136 kb on + strand, within ECOLIN_RS22140at 4447.136 kb on + strand, within ECOLIN_RS22140at 4447.216 kb on + strand, within ECOLIN_RS22140at 4447.217 kb on - strand, within ECOLIN_RS22140at 4447.217 kb on - strand, within ECOLIN_RS22140at 4447.221 kb on - strand, within ECOLIN_RS22140at 4447.259 kb on + strandat 4447.260 kb on - strandat 4447.260 kb on - strandat 4447.328 kb on + strandat 4447.359 kb on + strandat 4447.359 kb on + strandat 4447.360 kb on - strandat 4447.366 kb on + strandat 4447.366 kb on + strandat 4447.434 kb on - strandat 4447.434 kb on - strandat 4447.434 kb on - strandat 4447.458 kb on + strand, within ECOLIN_RS22145at 4447.459 kb on - strand, within ECOLIN_RS22145at 4447.526 kb on - strand, within ECOLIN_RS22145at 4447.553 kb on + strand, within ECOLIN_RS22145at 4447.553 kb on + strand, within ECOLIN_RS22145at 4447.554 kb on - strand, within ECOLIN_RS22145at 4447.712 kb on + strand, within ECOLIN_RS22145at 4447.713 kb on - strand, within ECOLIN_RS22145at 4447.742 kb on + strand, within ECOLIN_RS22145at 4447.742 kb on + strand, within ECOLIN_RS22145at 4447.743 kb on - strand, within ECOLIN_RS22145at 4448.055 kb on + strand, within ECOLIN_RS22145at 4448.068 kb on - strand, within ECOLIN_RS22145at 4448.068 kb on - strand, within ECOLIN_RS22145at 4448.068 kb on - strand, within ECOLIN_RS22145at 4448.068 kb on - strand, within ECOLIN_RS22145at 4448.190 kb on - strandat 4448.199 kb on - strandat 4448.217 kb on + strand, within ECOLIN_RS22150at 4448.218 kb on - strand, within ECOLIN_RS22150

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,445,712 + -0.4
4,445,712 + -0.6
4,445,717 + +0.2
4,445,717 + -0.0
4,445,717 + +0.0
4,445,718 - -0.1
4,445,718 - +0.0
4,445,718 - +0.1
4,445,718 - -0.2
4,445,718 - +1.7
4,445,762 + +0.2
4,445,762 + +0.4
4,445,763 - +0.5
4,445,789 - -0.4
4,445,812 + ECOLIN_RS22130 0.18 +0.2
4,445,812 + ECOLIN_RS22130 0.18 +0.2
4,445,812 + ECOLIN_RS22130 0.18 -0.3
4,446,289 + ECOLIN_RS22135 0.49 -0.8
4,446,321 + ECOLIN_RS22135 0.55 +0.3
4,446,321 + ECOLIN_RS22135 0.55 -0.5
4,446,321 + ECOLIN_RS22135 0.55 -0.1
4,446,322 - ECOLIN_RS22135 0.55 -0.8
4,446,542 + +0.2
4,446,561 + -0.1
4,446,561 + -0.7
4,446,562 - +0.4
4,446,659 + ECOLIN_RS22140 0.14 -1.4
4,446,678 - ECOLIN_RS22140 0.16 +0.1
4,446,678 - ECOLIN_RS22140 0.16 -0.6
4,446,781 + ECOLIN_RS22140 0.29 -0.1
4,446,781 + ECOLIN_RS22140 0.29 -0.9
4,446,851 + ECOLIN_RS22140 0.38 -0.2
4,446,851 + ECOLIN_RS22140 0.38 -5.0
4,446,852 - ECOLIN_RS22140 0.38 -0.4
4,446,853 + ECOLIN_RS22140 0.38 -0.6
4,446,908 + ECOLIN_RS22140 0.46 -0.6
4,447,017 + ECOLIN_RS22140 0.60 -0.0
4,447,017 + ECOLIN_RS22140 0.60 +0.3
4,447,017 + ECOLIN_RS22140 0.60 +0.1
4,447,017 + ECOLIN_RS22140 0.60 +0.2
4,447,017 + ECOLIN_RS22140 0.60 -1.9
4,447,018 - ECOLIN_RS22140 0.60 -1.3
4,447,136 + ECOLIN_RS22140 0.75 -0.1
4,447,136 + ECOLIN_RS22140 0.75 -1.4
4,447,216 + ECOLIN_RS22140 0.85 -1.3
4,447,217 - ECOLIN_RS22140 0.85 -0.6
4,447,217 - ECOLIN_RS22140 0.85 +0.2
4,447,221 - ECOLIN_RS22140 0.86 -1.2
4,447,259 + +0.2
4,447,260 - -0.1
4,447,260 - +0.1
4,447,328 + +0.2
4,447,359 + -0.0
4,447,359 + +0.2
4,447,360 - +0.4
4,447,366 + +1.2
4,447,366 + +0.3
4,447,434 - -0.0
4,447,434 - +0.1
4,447,434 - -0.1
4,447,458 + ECOLIN_RS22145 0.11 -0.0
4,447,459 - ECOLIN_RS22145 0.11 -0.3
4,447,526 - ECOLIN_RS22145 0.20 -0.3
4,447,553 + ECOLIN_RS22145 0.23 +0.1
4,447,553 + ECOLIN_RS22145 0.23 +0.3
4,447,554 - ECOLIN_RS22145 0.23 -0.1
4,447,712 + ECOLIN_RS22145 0.43 +0.3
4,447,713 - ECOLIN_RS22145 0.44 -0.2
4,447,742 + ECOLIN_RS22145 0.47 +0.4
4,447,742 + ECOLIN_RS22145 0.47 +0.1
4,447,743 - ECOLIN_RS22145 0.47 +0.5
4,448,055 + ECOLIN_RS22145 0.87 +0.4
4,448,068 - ECOLIN_RS22145 0.89 -0.0
4,448,068 - ECOLIN_RS22145 0.89 -0.4
4,448,068 - ECOLIN_RS22145 0.89 +0.3
4,448,068 - ECOLIN_RS22145 0.89 +0.4
4,448,190 - -0.4
4,448,199 - -0.2
4,448,217 + ECOLIN_RS22150 0.13 -0.4
4,448,218 - ECOLIN_RS22150 0.14 -0.8

Or see this region's nucleotide sequence