Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12700

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12690 and ECOLIN_RS12695 overlap by 4 nucleotidesECOLIN_RS12695 and ECOLIN_RS12700 are separated by 154 nucleotidesECOLIN_RS12700 and ECOLIN_RS12705 are separated by 222 nucleotides ECOLIN_RS12690: ECOLIN_RS12690 - sugar efflux transporter SetB, at 2,526,453 to 2,527,634 _RS12690 ECOLIN_RS12695: ECOLIN_RS12695 - YkgJ family cysteine cluster protein, at 2,527,631 to 2,527,885 _RS12695 ECOLIN_RS12700: ECOLIN_RS12700 - elongation factor P-like protein YeiP, at 2,528,040 to 2,528,612 _RS12700 ECOLIN_RS12705: ECOLIN_RS12705 - mannitol dehydrogenase family protein, at 2,528,835 to 2,530,301 _RS12705 Position (kb) 2528 2529Strain fitness (log2 ratio) -1 0 1 2 3at 2527.178 kb on + strand, within ECOLIN_RS12690at 2527.886 kb on - strandat 2529.068 kb on - strand, within ECOLIN_RS12705at 2529.068 kb on - strand, within ECOLIN_RS12705

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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2,527,178 + ECOLIN_RS12690 0.61 +1.1
2,527,886 - +2.9
2,529,068 - ECOLIN_RS12705 0.16 -0.0
2,529,068 - ECOLIN_RS12705 0.16 +0.3

Or see this region's nucleotide sequence