Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS03030

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS03025 and ECOLIN_RS03030 overlap by 4 nucleotidesECOLIN_RS03030 and ECOLIN_RS03035 are separated by 95 nucleotidesECOLIN_RS03035 and ECOLIN_RS03040 are separated by 110 nucleotides ECOLIN_RS03025: ECOLIN_RS03025 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 625,382 to 626,449 _RS03025 ECOLIN_RS03030: ECOLIN_RS03030 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 626,446 to 626,955 _RS03030 ECOLIN_RS03035: ECOLIN_RS03035 - hypothetical protein, at 627,051 to 627,380 _RS03035 ECOLIN_RS03040: ECOLIN_RS03040 - UDP-2,3-diacylglucosamine diphosphatase, at 627,491 to 628,213 _RS03040 Position (kb) 626 627Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 625.795 kb on - strand, within ECOLIN_RS03025at 625.982 kb on + strand, within ECOLIN_RS03025at 625.982 kb on + strand, within ECOLIN_RS03025at 627.315 kb on - strand, within ECOLIN_RS03035at 627.383 kb on + strandat 627.443 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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625,795 - ECOLIN_RS03025 0.39 -1.4
625,982 + ECOLIN_RS03025 0.56 -2.3
625,982 + ECOLIN_RS03025 0.56 -4.1
627,315 - ECOLIN_RS03035 0.80 -0.9
627,383 + +0.9
627,443 + +0.1

Or see this region's nucleotide sequence