Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS03020

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS03010 and ECOLIN_RS03015 are separated by 10 nucleotidesECOLIN_RS03015 and ECOLIN_RS03020 overlap by 4 nucleotidesECOLIN_RS03020 and ECOLIN_RS03025 are separated by 132 nucleotides ECOLIN_RS03010: ECOLIN_RS03010 - DUF1116 domain-containing protein, at 622,274 to 623,533 _RS03010 ECOLIN_RS03015: ECOLIN_RS03015 - DUF2877 domain-containing protein, at 623,544 to 624,359 _RS03015 ECOLIN_RS03020: ECOLIN_RS03020 - carbamate kinase, at 624,356 to 625,249 _RS03020 ECOLIN_RS03025: ECOLIN_RS03025 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 625,382 to 626,449 _RS03025 Position (kb) 624 625 626Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 624.182 kb on - strand, within ECOLIN_RS03015at 624.293 kb on + strandat 624.558 kb on + strand, within ECOLIN_RS03020at 624.579 kb on + strand, within ECOLIN_RS03020at 624.611 kb on + strand, within ECOLIN_RS03020at 624.773 kb on + strand, within ECOLIN_RS03020at 624.773 kb on + strand, within ECOLIN_RS03020at 625.037 kb on + strand, within ECOLIN_RS03020at 625.795 kb on - strand, within ECOLIN_RS03025at 625.982 kb on + strand, within ECOLIN_RS03025at 625.982 kb on + strand, within ECOLIN_RS03025

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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624,182 - ECOLIN_RS03015 0.78 +0.8
624,293 + +0.1
624,558 + ECOLIN_RS03020 0.23 +0.1
624,579 + ECOLIN_RS03020 0.25 -0.4
624,611 + ECOLIN_RS03020 0.29 +0.7
624,773 + ECOLIN_RS03020 0.47 +0.2
624,773 + ECOLIN_RS03020 0.47 -0.4
625,037 + ECOLIN_RS03020 0.76 -0.5
625,795 - ECOLIN_RS03025 0.39 -1.4
625,982 + ECOLIN_RS03025 0.56 -2.3
625,982 + ECOLIN_RS03025 0.56 -4.1

Or see this region's nucleotide sequence